FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308593976> ?p ?o ?g. }

• W4308593976 abstract "The liver immune microenvironment is a key element in the development of hepatic inflammation in NAFLD. ApoA4 deficiency increases the hepatic lipid burden, insulin resistance, and metabolic inflammation. However, the effect of ApoA4 on liver immune cells and the precise immune cell subsets that exacerbate fatty liver remain elusive. The aim of this study was to profile the hepatic immune cells affected by ApoA4 in NAFL. We performed scRNA-seq on liver immune cells from WT and ApoA4 -deficient mice administered a high-fat diet. Immunostaining and qRT–PCR analysis were used to validate the results of scRNA-seq. We identified 10 discrete immune cell populations comprising macrophages, DCs, granulocytes, B, T and NK&amp;NKT cells and characterized their subsets, gene expression profiles, and functional modules. ApoA4 deficiency led to significant increases in the abundance of specific subsets, including inflammatory macrophages (2-Mφ-Cxcl9 and 4-Mφ-Cxcl2) and activated granulocytes (0-Gran-Wfdc17). Moreover, ApoA4 deficiency resulted in higher Lgals3 , Ctss , Fcgr2b , Spp1 , Cxcl2 , and Elane levels and lower Nr4a1 levels in hepatic immune cells. These genes were consistent with human NAFLD-associated marker genes linked to disease severity. The expression of NE and IL-1β in granulocytes and macrophages as key ApoA4 targets were validate in the presence or absence of ApoA4 by immunostaining. The scRNA-seq data analyses revealed reprogramming of liver immune cells resulted from ApoA4 deficiency. We uncovered that the emergence of ApoA4-associated immune subsets (namely Cxcl9 + macrophage, Cxcl2 + macrophage and Wfdc17 + granulocyte), pathways, and NAFLD-related marker genes may promote the development of NAFL. These findings may provide novel therapeutic targets for NAFL and the foundations for further studying the effects of ApoA4 on immune cells in various diseases." @default.
• W4308593976 created "2022-11-12" @default.
• W4308593976 creator A5012578254 @default.
• W4308593976 creator A5014261420 @default.
• W4308593976 creator A5020088480 @default.
• W4308593976 creator A5030135901 @default.
• W4308593976 creator A5033998172 @default.
• W4308593976 creator A5042518838 @default.
• W4308593976 creator A5046202286 @default.
• W4308593976 creator A5056837726 @default.
• W4308593976 creator A5084461414 @default.
• W4308593976 creator A5087945074 @default.
• W4308593976 creator A5088673743 @default.
• W4308593976 date "2022-11-08" @default.
• W4308593976 modified "2023-10-01" @default.
• W4308593976 title "Single-cell RNA sequencing reveals a novel inhibitory effect of ApoA4 on NAFL mediated by liver-specific subsets of myeloid cells" @default.
• W4308593976 cites W1753997046 @default.
• W4308593976 cites W1986132627 @default.
• W4308593976 cites W2007197271 @default.
• W4308593976 cites W2007663694 @default.
• W4308593976 cites W2019954526 @default.
• W4308593976 cites W2035254458 @default.
• W4308593976 cites W2035618305 @default.
• W4308593976 cites W2048992783 @default.
• W4308593976 cites W2053142936 @default.
• W4308593976 cites W2129116220 @default.
• W4308593976 cites W2130410032 @default.
• W4308593976 cites W2151362823 @default.
• W4308593976 cites W2153846543 @default.
• W4308593976 cites W2268066121 @default.
• W4308593976 cites W2273689975 @default.
• W4308593976 cites W2409873454 @default.
• W4308593976 cites W2472000879 @default.
• W4308593976 cites W2514873437 @default.
• W4308593976 cites W2561860468 @default.
• W4308593976 cites W2580918896 @default.
• W4308593976 cites W2582611686 @default.
• W4308593976 cites W2592963267 @default.
• W4308593976 cites W2599822883 @default.
• W4308593976 cites W2747181438 @default.
• W4308593976 cites W2792855672 @default.
• W4308593976 cites W2796883066 @default.
• W4308593976 cites W2800885569 @default.
• W4308593976 cites W2810423511 @default.
• W4308593976 cites W2888894833 @default.
• W4308593976 cites W2894854766 @default.
• W4308593976 cites W2898012255 @default.
• W4308593976 cites W2947512282 @default.
• W4308593976 cites W2953698023 @default.
• W4308593976 cites W2968872536 @default.
• W4308593976 cites W2979371456 @default.
• W4308593976 cites W2982638381 @default.
• W4308593976 cites W2998756962 @default.
• W4308593976 cites W3014824156 @default.
• W4308593976 cites W3016874961 @default.
• W4308593976 cites W3019068524 @default.
• W4308593976 cites W3023775962 @default.
• W4308593976 cites W3043246820 @default.
• W4308593976 cites W3044190151 @default.
• W4308593976 cites W3046258938 @default.
• W4308593976 cites W3082104408 @default.
• W4308593976 cites W3086548448 @default.
• W4308593976 cites W3107674236 @default.
• W4308593976 cites W3113558414 @default.
• W4308593976 cites W3117083078 @default.
• W4308593976 cites W3132661792 @default.
• W4308593976 cites W3147310958 @default.
• W4308593976 cites W3157384750 @default.
• W4308593976 cites W3165524514 @default.
• W4308593976 cites W3173330235 @default.
• W4308593976 cites W4289174733 @default.
• W4308593976 doi "https://doi.org/10.3389/fimmu.2022.1038401" @default.
• W4308593976 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36426356" @default.
• W4308593976 hasPublicationYear "2022" @default.
• W4308593976 type Work @default.
• W4308593976 citedByCount "1" @default.
• W4308593976 countsByYear W43085939762022 @default.
• W4308593976 crossrefType "journal-article" @default.
• W4308593976 hasAuthorship W4308593976A5012578254 @default.
• W4308593976 hasAuthorship W4308593976A5014261420 @default.
• W4308593976 hasAuthorship W4308593976A5020088480 @default.
• W4308593976 hasAuthorship W4308593976A5030135901 @default.
• W4308593976 hasAuthorship W4308593976A5033998172 @default.
• W4308593976 hasAuthorship W4308593976A5042518838 @default.
• W4308593976 hasAuthorship W4308593976A5046202286 @default.
• W4308593976 hasAuthorship W4308593976A5056837726 @default.
• W4308593976 hasAuthorship W4308593976A5084461414 @default.
• W4308593976 hasAuthorship W4308593976A5087945074 @default.
• W4308593976 hasAuthorship W4308593976A5088673743 @default.
• W4308593976 hasBestOaLocation W43085939761 @default.
• W4308593976 hasConcept C12823836 @default.
• W4308593976 hasConcept C13373296 @default.
• W4308593976 hasConcept C136449434 @default.
• W4308593976 hasConcept C179661763 @default.
• W4308593976 hasConcept C203014093 @default.
• W4308593976 hasConcept C2776090121 @default.
• W4308593976 hasConcept C2776914184 @default.
• W4308593976 hasConcept C86803240 @default.
• W4308593976 hasConcept C8891405 @default.
• W4308593976 hasConcept C95444343 @default.

• next