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- W4308652847 abstract "Usutu virus (USUV) is a mosquito-borne zoonotic virus and one of the causes of flavivirus encephalitis in birds and occasionally in humans. USUV rapidly disperses in a susceptible host and vector environment, as is the case in South and Central Europe. However, compared to other flaviviruses, USUV has received less research attention and there is therefore limited access to whole-genome sequences and also to in-depth phylogenetic and phylodynamic analyses. To ease future molecular studies, this study compares first- (partial sequencing via Sanger), second- (Illumina), and third-generation (MinION Nanopore) sequencing platforms for USUV. With emphasis on MinION Nanopore sequencing, cDNA-direct and target-enrichment (amplicon-based) sequencing approaches were validated in parallel. The study was based on four samples from succumbed birds commonly collected throughout Germany. The samples were isolated from various sample matrices, organs as well as blood cruor, and included three different USUV lineages. We concluded that depending on the focus of a research project, amplicon-based MinION Nanopore sequencing can be an ideal cost- and time-effective alternative to Illumina in producing optimal genome coverage. It can be implemented for an array of lab- or field-based objectives, including among others: phylodynamic studies and the analysis of viral quasispecies." @default.
- W4308652847 created "2022-11-13" @default.
- W4308652847 creator A5015405869 @default.
- W4308652847 creator A5029181753 @default.
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- W4308652847 creator A5034905489 @default.
- W4308652847 creator A5041531967 @default.
- W4308652847 creator A5078928217 @default.
- W4308652847 creator A5080054557 @default.
- W4308652847 date "2022-11-09" @default.
- W4308652847 modified "2023-10-13" @default.
- W4308652847 title "Expedited retrieval of high-quality Usutu virus genomes via Nanopore sequencing with and without target enrichment" @default.
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