FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308686978> ?p ?o ?g. }

• W4308686978 endingPage "9" @default.
• W4308686978 startingPage "1" @default.
• W4308686978 abstract "We studied the effects of computed tomography (CT) scan irradiation on proliferation and differentiation of human embryonic stem cells (hESCs). It was reported that hESC is extremely radiosensitive; exposure of hESC in cultures to 1 Gy of ionizing radiation (IR) results in massive apoptosis of the damaged cells and, thus, they are eliminated from the cultures. However, after recovery the surviving cells proliferate and differentiate normally. We hypothesized that IR-exposed hESC may still have growth rate disadvantage when they proliferate or differentiate in the presence of non-irradiated hESC, as has been shown for mouse hematopoietic stem cells in vivo.To study such competitive proliferation and differentiation, we obtained cells of H9 hESC line that stably express green fluorescent protein (H9GFP). Irradiated with 50 mGy or 500 mGy H9GFP and non-irradiated H9 cells (or vice versa) were mixed and allowed to grow under pluripotency maintaining conditions or under conditions of directed differentiation into neuronal lineage for several passages. The ratio of H9GFP to H9 cells was measured after every passage or approximately every week.We observed competition of H9 and H9GFP cells; we found that the ratio of H9GFP to H9 cells increased with time in both proliferation and differentiation conditions regardless of irradiation, i.e. the H9GFP cells in general grew faster than H9 cells in the mixtures. However, we did not observe any consistent changes in the relative growth rate of irradiated versus non-irradiated hESC.We conclude that population of pluripotent hESC is very resilient; while damaged cells are eliminated from colonies, the surviving cells retain their pluripotency, ability to differentiate, and compete with non-irradiated isogenic cells. These findings are consistent with the results of our previous studies, and with the concept that early in pregnancy omnipotent cells injured by IR can be replaced by non-damaged cells with no impact on embryo development." @default.
• W4308686978 created "2022-11-14" @default.
• W4308686978 creator A5006866029 @default.
• W4308686978 creator A5029638399 @default.
• W4308686978 creator A5048669413 @default.
• W4308686978 creator A5053934339 @default.
• W4308686978 creator A5056579080 @default.
• W4308686978 creator A5070135020 @default.
• W4308686978 date "2022-12-07" @default.
• W4308686978 modified "2023-09-26" @default.
• W4308686978 title "Effect of exposure to ionizing radiation on competitive proliferation and differentiation of hESC" @default.
• W4308686978 cites W1496422544 @default.
• W4308686978 cites W1964351261 @default.
• W4308686978 cites W1966056603 @default.
• W4308686978 cites W2005225541 @default.
• W4308686978 cites W2013771570 @default.
• W4308686978 cites W2042116803 @default.
• W4308686978 cites W2057487406 @default.
• W4308686978 cites W2065146977 @default.
• W4308686978 cites W2088048548 @default.
• W4308686978 cites W2090190697 @default.
• W4308686978 cites W2101227721 @default.
• W4308686978 cites W2117899512 @default.
• W4308686978 cites W2138885078 @default.
• W4308686978 cites W2163067447 @default.
• W4308686978 cites W2169423456 @default.
• W4308686978 cites W2169867898 @default.
• W4308686978 cites W2210538740 @default.
• W4308686978 cites W2298057923 @default.
• W4308686978 cites W2411511262 @default.
• W4308686978 cites W2414438694 @default.
• W4308686978 cites W2525290462 @default.
• W4308686978 cites W2564913300 @default.
• W4308686978 cites W2590236471 @default.
• W4308686978 cites W2592815136 @default.
• W4308686978 cites W2754433473 @default.
• W4308686978 cites W2900228461 @default.
• W4308686978 cites W2943756610 @default.
• W4308686978 cites W2949277673 @default.
• W4308686978 cites W2967490671 @default.
• W4308686978 cites W3013772145 @default.
• W4308686978 cites W3016781574 @default.
• W4308686978 cites W3047038020 @default.
• W4308686978 doi "https://doi.org/10.1080/09553002.2023.2146231" @default.
• W4308686978 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36352506" @default.
• W4308686978 hasPublicationYear "2022" @default.
• W4308686978 type Work @default.
• W4308686978 citedByCount "0" @default.
• W4308686978 crossrefType "journal-article" @default.
• W4308686978 hasAuthorship W4308686978A5006866029 @default.
• W4308686978 hasAuthorship W4308686978A5029638399 @default.
• W4308686978 hasAuthorship W4308686978A5048669413 @default.
• W4308686978 hasAuthorship W4308686978A5053934339 @default.
• W4308686978 hasAuthorship W4308686978A5056579080 @default.
• W4308686978 hasAuthorship W4308686978A5070135020 @default.
• W4308686978 hasConcept C104317684 @default.
• W4308686978 hasConcept C109159458 @default.
• W4308686978 hasConcept C111337013 @default.
• W4308686978 hasConcept C121332964 @default.
• W4308686978 hasConcept C145103041 @default.
• W4308686978 hasConcept C148738053 @default.
• W4308686978 hasConcept C153911025 @default.
• W4308686978 hasConcept C18231593 @default.
• W4308686978 hasConcept C185544564 @default.
• W4308686978 hasConcept C28328180 @default.
• W4308686978 hasConcept C2908647359 @default.
• W4308686978 hasConcept C54355233 @default.
• W4308686978 hasConcept C71924100 @default.
• W4308686978 hasConcept C86803240 @default.
• W4308686978 hasConcept C95444343 @default.
• W4308686978 hasConcept C99454951 @default.
• W4308686978 hasConceptScore W4308686978C104317684 @default.
• W4308686978 hasConceptScore W4308686978C109159458 @default.
• W4308686978 hasConceptScore W4308686978C111337013 @default.
• W4308686978 hasConceptScore W4308686978C121332964 @default.
• W4308686978 hasConceptScore W4308686978C145103041 @default.
• W4308686978 hasConceptScore W4308686978C148738053 @default.
• W4308686978 hasConceptScore W4308686978C153911025 @default.
• W4308686978 hasConceptScore W4308686978C18231593 @default.
• W4308686978 hasConceptScore W4308686978C185544564 @default.
• W4308686978 hasConceptScore W4308686978C28328180 @default.
• W4308686978 hasConceptScore W4308686978C2908647359 @default.
• W4308686978 hasConceptScore W4308686978C54355233 @default.
• W4308686978 hasConceptScore W4308686978C71924100 @default.
• W4308686978 hasConceptScore W4308686978C86803240 @default.
• W4308686978 hasConceptScore W4308686978C95444343 @default.
• W4308686978 hasConceptScore W4308686978C99454951 @default.
• W4308686978 hasLocation W43086869781 @default.
• W4308686978 hasLocation W43086869782 @default.
• W4308686978 hasOpenAccess W4308686978 @default.
• W4308686978 hasPrimaryLocation W43086869781 @default.
• W4308686978 hasRelatedWork W1978835772 @default.
• W4308686978 hasRelatedWork W1996916774 @default.
• W4308686978 hasRelatedWork W2009519731 @default.
• W4308686978 hasRelatedWork W2053223017 @default.
• W4308686978 hasRelatedWork W2119511363 @default.
• W4308686978 hasRelatedWork W2120223845 @default.
• W4308686978 hasRelatedWork W2137903841 @default.

• next