Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308694755> ?p ?o ?g. }
- W4308694755 endingPage "2885" @default.
- W4308694755 startingPage "2885" @default.
- W4308694755 abstract "Chimeric antigen receptor (CAR) immunotherapy includes the genetic modification of immune cells to carry such a receptor and, thus, recognize cancer cell surface antigens. Viral transfection is currently the preferred method, but it carries the risk of off-target mutagenicity. Other transfection platforms have thus been proposed, such the in vitro transcribed (IVT)-mRNAs. In this study, we exploited our innovative, patented delivery platform to produce protein transduction domain (PTD)-IVT-mRNAs for the expression of CAR on NK-92 cells. CAR T1E-engineered NK-92 cells, harboring the sequence of T1E single-chain fragment variant (scFv) to recognize the ErbB receptor, bearing either CD28 or 4-1BB as co-stimulatory signaling domains, were prepared and assessed for their effectiveness in two different ErbB(+) cancer cell lines. Our results showed that the PTD-IVT-mRNA of CAR was safely transduced and expressed into NK-92 cells. CAR T1E-engineered NK-92 cells provoked high levels of cell death (25-33%) as effector cells against both HSC-3 (oral squamous carcinoma) and MCF-7 (breast metastatic adenocarcinoma) human cells in the co-incubation assays. In conclusion, the application of our novel PTD-IVT-mRNA delivery platform to NK-92 cells gave promising results towards future CAR immunotherapy approaches." @default.
- W4308694755 created "2022-11-14" @default.
- W4308694755 creator A5003206659 @default.
- W4308694755 creator A5011513927 @default.
- W4308694755 creator A5014813526 @default.
- W4308694755 creator A5021629166 @default.
- W4308694755 creator A5037949019 @default.
- W4308694755 date "2022-11-10" @default.
- W4308694755 modified "2023-09-26" @default.
- W4308694755 title "An Innovative PTD-IVT-mRNA Delivery Platform for CAR Immunotherapy of ErbB(+) Solid Tumor Neoplastic Cells" @default.
- W4308694755 cites W1215958058 @default.
- W4308694755 cites W1531431054 @default.
- W4308694755 cites W1573342219 @default.
- W4308694755 cites W1942293727 @default.
- W4308694755 cites W1972263472 @default.
- W4308694755 cites W1984287161 @default.
- W4308694755 cites W1985600039 @default.
- W4308694755 cites W1988142400 @default.
- W4308694755 cites W2008447696 @default.
- W4308694755 cites W2018289835 @default.
- W4308694755 cites W2025980739 @default.
- W4308694755 cites W2047205338 @default.
- W4308694755 cites W2053281420 @default.
- W4308694755 cites W2057466800 @default.
- W4308694755 cites W2091849351 @default.
- W4308694755 cites W2100837269 @default.
- W4308694755 cites W2111034570 @default.
- W4308694755 cites W2111276564 @default.
- W4308694755 cites W2121626779 @default.
- W4308694755 cites W2122678757 @default.
- W4308694755 cites W2130871532 @default.
- W4308694755 cites W2133498548 @default.
- W4308694755 cites W2134812217 @default.
- W4308694755 cites W2143435540 @default.
- W4308694755 cites W2162165801 @default.
- W4308694755 cites W2164598295 @default.
- W4308694755 cites W2285239684 @default.
- W4308694755 cites W2298503769 @default.
- W4308694755 cites W2339327563 @default.
- W4308694755 cites W2399140547 @default.
- W4308694755 cites W2399876335 @default.
- W4308694755 cites W2467116108 @default.
- W4308694755 cites W2555169881 @default.
- W4308694755 cites W2560367415 @default.
- W4308694755 cites W2615682320 @default.
- W4308694755 cites W2753647590 @default.
- W4308694755 cites W2754562068 @default.
- W4308694755 cites W2756128996 @default.
- W4308694755 cites W2769297636 @default.
- W4308694755 cites W2769847998 @default.
- W4308694755 cites W2775390426 @default.
- W4308694755 cites W2790863559 @default.
- W4308694755 cites W2800948756 @default.
- W4308694755 cites W2801097243 @default.
- W4308694755 cites W2805378765 @default.
- W4308694755 cites W2892333029 @default.
- W4308694755 cites W2898951783 @default.
- W4308694755 cites W2903777694 @default.
- W4308694755 cites W2942874616 @default.
- W4308694755 cites W2945863457 @default.
- W4308694755 cites W2963067921 @default.
- W4308694755 cites W2971495812 @default.
- W4308694755 cites W2984209785 @default.
- W4308694755 cites W29899406 @default.
- W4308694755 cites W2992445625 @default.
- W4308694755 cites W2999031113 @default.
- W4308694755 cites W2999534343 @default.
- W4308694755 cites W3002899364 @default.
- W4308694755 cites W3033639125 @default.
- W4308694755 cites W3035944244 @default.
- W4308694755 cites W3046926665 @default.
- W4308694755 cites W3048499403 @default.
- W4308694755 cites W3080712767 @default.
- W4308694755 cites W3112054237 @default.
- W4308694755 cites W3122724341 @default.
- W4308694755 cites W3124695109 @default.
- W4308694755 cites W3124760272 @default.
- W4308694755 cites W3128879876 @default.
- W4308694755 cites W3156114568 @default.
- W4308694755 cites W3185943014 @default.
- W4308694755 cites W3193885942 @default.
- W4308694755 cites W3201340030 @default.
- W4308694755 cites W4200016445 @default.
- W4308694755 cites W4200565167 @default.
- W4308694755 cites W4210589143 @default.
- W4308694755 cites W4220721336 @default.
- W4308694755 cites W4221022899 @default.
- W4308694755 cites W4221130391 @default.
- W4308694755 cites W4280649059 @default.
- W4308694755 cites W4290706026 @default.
- W4308694755 cites W4293802417 @default.
- W4308694755 cites W4294216491 @default.
- W4308694755 cites W4303579455 @default.
- W4308694755 cites W88850206 @default.
- W4308694755 doi "https://doi.org/10.3390/biomedicines10112885" @default.
- W4308694755 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36359405" @default.
- W4308694755 hasPublicationYear "2022" @default.
- W4308694755 type Work @default.