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- W4308703546 abstract "Abstract During autophagy, rapid membrane assembly expands small phagophores into large double-membrane autophagosomes. Theoretical modelling predicts the majority of autophagosomal phospholipids is derived from highly efficient non-vesicular phospholipid transfer (PLT) across phagophore-ER contacts (PERCS). Currently, the phagophore-ER tether Atg2 is the only PLT protein known to drive phagophore expansion in vivo . Here, our quantitative live-cell-imaging analysis reveals poor correlation between duration and size of forming autophagosomes and number of Atg2 molecules at PERCS of starving yeast cells. Strikingly, we find Atg2-mediated PLT is non-rate-limiting for autophagosome biogenesis, because membrane tether and PLT protein Vps13 localizes to the rim and promotes expansion of phagophores in parallel with Atg2. In the absence of Vps13, the number of Atg2 molecules at PERCS determines duration and size of forming autophagosomes with an apparent in vivo transfer rate of ~200 phospholipids per Atg2 molecule and second. We propose conserved PLT proteins cooperate in channeling phospholipids across organelle contact sites for non-rate-limiting membrane assembly during autophagosome biogenesis." @default.
- W4308703546 created "2022-11-14" @default.
- W4308703546 creator A5009827959 @default.
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- W4308703546 date "2022-11-10" @default.
- W4308703546 modified "2023-09-29" @default.
- W4308703546 title "Parallel phospholipid transfer by Vps13 and Atg2 determines autophagosome biogenesis dynamics" @default.
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- W4308703546 doi "https://doi.org/10.1101/2022.11.10.516013" @default.
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