FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308746064> ?p ?o ?g. }

• W4308746064 endingPage "147034" @default.
• W4308746064 startingPage "147034" @default.
• W4308746064 abstract "The Hsp18 protein is a major T-cell antigen of Mycobacterium leprae belonging to the family of small heat-shock proteins. The protein is specifically regulated at post-translational level during the intracellular growth of M. leprae within macrophages due to auto-phosphorylation, indicating its importance in the survival of the bacterium. The promoter and regulatory sequences that control hsp18 expression are located within a 256-bp sequence upstream of the translation start site. However, there are no studies describing either characterization of the hsp18 promoter or its genetic regulation. Therefore, we constructed an hsp18-EGFP transcriptional fusion in an E. coli–Mycobacterium shuttle vector. A 168-bp sequence comprising the hsp18 promoter was cloned upstream of the EGFP gene and transformed in M. smegmatis, and the integration of the construct was confirmed by Southern hybridization. hsp18 promoter activity was measured by analyzing EGFP expression in M. smegmatis and Escherichia coli grown under different environmental stress conditions normally encountered by M. leprae in vivo. We found that the 168-bp upstream sequence of hsp18 could function as a promoter, and the regulation of hsp18 expression was host-, environmental stress-, and temperature-dependent. Appreciable EGFP expression was detected in M. smegmatis grown under normal conditions, and theexpression was significantly increased by environmental stress. However, EGFP expression was observed in E. coli only under stress conditions. Comparative sequence analysis revealed the putative sigma factor C (SigC)-binding site within the 168-bp promoter sequence of hsp18, which might be involved in the regulation of hsp18 expression during stress conditions in M. leprae. Thus, our data demonstrated the transcriptional regulation of hsp18 expression in response to different environmental stress conditions, possibly through SigC in Mycobacterium. Further, this shuttle vector could be used for the functional characterization of M. leprae genes in heterologous systems." @default.
• W4308746064 created "2022-11-15" @default.
• W4308746064 creator A5007991211 @default.
• W4308746064 creator A5037081091 @default.
• W4308746064 creator A5054217274 @default.
• W4308746064 creator A5057500275 @default.
• W4308746064 creator A5075589540 @default.
• W4308746064 date "2023-01-01" @default.
• W4308746064 modified "2023-10-15" @default.
• W4308746064 title "Mycobacterium leprae hsp18 promoter-EGFP transcriptional fusion construct: Environmental stress and strain-specific expression" @default.
• W4308746064 cites W1514631861 @default.
• W4308746064 cites W1517406463 @default.
• W4308746064 cites W1549522998 @default.
• W4308746064 cites W1562347299 @default.
• W4308746064 cites W1597808424 @default.
• W4308746064 cites W1626718189 @default.
• W4308746064 cites W1827140628 @default.
• W4308746064 cites W1851138068 @default.
• W4308746064 cites W1889052494 @default.
• W4308746064 cites W1901536863 @default.
• W4308746064 cites W1967457339 @default.
• W4308746064 cites W1970697372 @default.
• W4308746064 cites W1972880328 @default.
• W4308746064 cites W1975813544 @default.
• W4308746064 cites W1986175940 @default.
• W4308746064 cites W2013287155 @default.
• W4308746064 cites W2014669590 @default.
• W4308746064 cites W2016035433 @default.
• W4308746064 cites W2017539316 @default.
• W4308746064 cites W2033805385 @default.
• W4308746064 cites W2063450941 @default.
• W4308746064 cites W2065833143 @default.
• W4308746064 cites W2066857552 @default.
• W4308746064 cites W2070698634 @default.
• W4308746064 cites W2079109812 @default.
• W4308746064 cites W2086677897 @default.
• W4308746064 cites W2100837269 @default.
• W4308746064 cites W2102986104 @default.
• W4308746064 cites W2103314018 @default.
• W4308746064 cites W2109872688 @default.
• W4308746064 cites W2114206141 @default.
• W4308746064 cites W2137988454 @default.
• W4308746064 cites W2153943457 @default.
• W4308746064 cites W2154003425 @default.
• W4308746064 cites W2158023121 @default.
• W4308746064 cites W2164217177 @default.
• W4308746064 cites W2168653518 @default.
• W4308746064 cites W2224929038 @default.
• W4308746064 cites W4293247451 @default.
• W4308746064 doi "https://doi.org/10.1016/j.gene.2022.147034" @default.
• W4308746064 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36371000" @default.
• W4308746064 hasPublicationYear "2023" @default.
• W4308746064 type Work @default.
• W4308746064 citedByCount "0" @default.
• W4308746064 crossrefType "journal-article" @default.
• W4308746064 hasAuthorship W4308746064A5007991211 @default.
• W4308746064 hasAuthorship W4308746064A5037081091 @default.
• W4308746064 hasAuthorship W4308746064A5054217274 @default.
• W4308746064 hasAuthorship W4308746064A5057500275 @default.
• W4308746064 hasAuthorship W4308746064A5075589540 @default.
• W4308746064 hasConcept C101762097 @default.
• W4308746064 hasConcept C104317684 @default.
• W4308746064 hasConcept C123894998 @default.
• W4308746064 hasConcept C142613039 @default.
• W4308746064 hasConcept C142724271 @default.
• W4308746064 hasConcept C150194340 @default.
• W4308746064 hasConcept C153911025 @default.
• W4308746064 hasConcept C192245184 @default.
• W4308746064 hasConcept C2777204207 @default.
• W4308746064 hasConcept C2777975735 @default.
• W4308746064 hasConcept C2781069245 @default.
• W4308746064 hasConcept C40767141 @default.
• W4308746064 hasConcept C54355233 @default.
• W4308746064 hasConcept C71924100 @default.
• W4308746064 hasConcept C86803240 @default.
• W4308746064 hasConceptScore W4308746064C101762097 @default.
• W4308746064 hasConceptScore W4308746064C104317684 @default.
• W4308746064 hasConceptScore W4308746064C123894998 @default.
• W4308746064 hasConceptScore W4308746064C142613039 @default.
• W4308746064 hasConceptScore W4308746064C142724271 @default.
• W4308746064 hasConceptScore W4308746064C150194340 @default.
• W4308746064 hasConceptScore W4308746064C153911025 @default.
• W4308746064 hasConceptScore W4308746064C192245184 @default.
• W4308746064 hasConceptScore W4308746064C2777204207 @default.
• W4308746064 hasConceptScore W4308746064C2777975735 @default.
• W4308746064 hasConceptScore W4308746064C2781069245 @default.
• W4308746064 hasConceptScore W4308746064C40767141 @default.
• W4308746064 hasConceptScore W4308746064C54355233 @default.
• W4308746064 hasConceptScore W4308746064C71924100 @default.
• W4308746064 hasConceptScore W4308746064C86803240 @default.
• W4308746064 hasFunder F4320320717 @default.
• W4308746064 hasFunder F4320320719 @default.
• W4308746064 hasLocation W43087460641 @default.
• W4308746064 hasLocation W43087460642 @default.
• W4308746064 hasOpenAccess W4308746064 @default.
• W4308746064 hasPrimaryLocation W43087460641 @default.
• W4308746064 hasRelatedWork W2009966535 @default.
• W4308746064 hasRelatedWork W2150397055 @default.

• next