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- W4308751411 abstract "A significant hurdle to understanding the functions of deubiquitinases (DUBs) is the identification of their in vivo substrates. Substrate identification can be difficult for two reasons. First, many proteins that are degraded by the ubiquitin–proteasome system are expressed at relatively low levels in the cell, and second, redundancy between DUBs complicates loss of function screening approaches. Here, we describe a systematic overexpression approach that takes advantage of genome-wide resources available in S. cerevisiae to overcome these challenges and identify DUB substrates in cells." @default.
- W4308751411 created "2022-11-15" @default.
- W4308751411 creator A5010168521 @default.
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- W4308751411 date "2022-11-10" @default.
- W4308751411 modified "2023-09-30" @default.
- W4308751411 title "Identification of Deubiquitinase Substrates in Saccharomyces cerevisiae by Systematic Overexpression" @default.
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- W4308751411 doi "https://doi.org/10.1007/978-1-0716-2803-4_14" @default.
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