Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308808416> ?p ?o ?g. }
- W4308808416 endingPage "10" @default.
- W4308808416 startingPage "1" @default.
- W4308808416 abstract "This study aimed to determine the ability of bacteria to produce the chitinase enzyme, purify, and characterize the enzyme from the isolate with the best activity, and determine the use of this purified enzyme as a biocontrol agent. The chitinolytic bacterium was identified as Stenotrophomonas maltophilia. The chitinase enzyme was purified 1.4 times at a 30% ammonium sulfate concentration with a yield of 40.7%. Following partial purification, the enzyme was purified by ion-exchange chromatography using HiPrep Q XL 16/10 column and HiPrep™ 26/10 desalting column with 25.34% and 18.12% yields, respectively. It was calculated that the purified enzyme had a molecular weight of 52 kDa by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. The optimum activity of the enzyme was determined at 50 °C and pH 7.0. Enzyme activity was most induced by Fe2+, while it was most inhibited by Zn2+ at 5 mM concentration. Km and Vmax values of the enzyme for colloidal chitin were calculated as 1.6419 mg/mL and 16.129 U/mg, respectively. The purified chitinase was used as a biocontrol agent against the fungus Fusarium oxysporum and potato beetle Leptinotarsa decemlineata. The enzyme was shown to be effective in reducing the growth of fungus and causing disruption of the chitin structure of potato beetle." @default.
- W4308808416 created "2022-11-15" @default.
- W4308808416 creator A5024352452 @default.
- W4308808416 creator A5036657345 @default.
- W4308808416 creator A5049489177 @default.
- W4308808416 creator A5072826105 @default.
- W4308808416 date "2022-11-11" @default.
- W4308808416 modified "2023-10-05" @default.
- W4308808416 title "Purification and characterization of <i>Stenotrophomonas maltophilia</i> chitinase with antifungal and insecticidal properties" @default.
- W4308808416 cites W1154298487 @default.
- W4308808416 cites W1486610421 @default.
- W4308808416 cites W1775749144 @default.
- W4308808416 cites W1963757655 @default.
- W4308808416 cites W1970156586 @default.
- W4308808416 cites W1973912186 @default.
- W4308808416 cites W1978108133 @default.
- W4308808416 cites W1980701408 @default.
- W4308808416 cites W1981661290 @default.
- W4308808416 cites W1984909890 @default.
- W4308808416 cites W1993385539 @default.
- W4308808416 cites W1999848111 @default.
- W4308808416 cites W2000187940 @default.
- W4308808416 cites W2000801278 @default.
- W4308808416 cites W2020392492 @default.
- W4308808416 cites W2030530955 @default.
- W4308808416 cites W2039733479 @default.
- W4308808416 cites W2043976052 @default.
- W4308808416 cites W2048584507 @default.
- W4308808416 cites W2054979333 @default.
- W4308808416 cites W2074979832 @default.
- W4308808416 cites W2078774499 @default.
- W4308808416 cites W2079117547 @default.
- W4308808416 cites W2086213992 @default.
- W4308808416 cites W2086992613 @default.
- W4308808416 cites W2093116210 @default.
- W4308808416 cites W2100837269 @default.
- W4308808416 cites W2101293392 @default.
- W4308808416 cites W2123224153 @default.
- W4308808416 cites W2134673353 @default.
- W4308808416 cites W2138196940 @default.
- W4308808416 cites W2139176007 @default.
- W4308808416 cites W2144275021 @default.
- W4308808416 cites W2156760556 @default.
- W4308808416 cites W2201787692 @default.
- W4308808416 cites W2287949950 @default.
- W4308808416 cites W2396868261 @default.
- W4308808416 cites W2414056474 @default.
- W4308808416 cites W2422457666 @default.
- W4308808416 cites W2754964410 @default.
- W4308808416 cites W2804631021 @default.
- W4308808416 cites W2809452373 @default.
- W4308808416 cites W2884772428 @default.
- W4308808416 cites W2910486838 @default.
- W4308808416 cites W2996143055 @default.
- W4308808416 cites W3007237000 @default.
- W4308808416 cites W3025697756 @default.
- W4308808416 cites W4200577347 @default.
- W4308808416 cites W4221005555 @default.
- W4308808416 cites W4236238089 @default.
- W4308808416 doi "https://doi.org/10.1080/10826068.2022.2142942" @default.
- W4308808416 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36369794" @default.
- W4308808416 hasPublicationYear "2022" @default.
- W4308808416 type Work @default.
- W4308808416 citedByCount "3" @default.
- W4308808416 countsByYear W43088084162023 @default.
- W4308808416 crossrefType "journal-article" @default.
- W4308808416 hasAuthorship W4308808416A5024352452 @default.
- W4308808416 hasAuthorship W4308808416A5036657345 @default.
- W4308808416 hasAuthorship W4308808416A5049489177 @default.
- W4308808416 hasAuthorship W4308808416A5072826105 @default.
- W4308808416 hasBestOaLocation W43088084162 @default.
- W4308808416 hasConcept C101660505 @default.
- W4308808416 hasConcept C116084860 @default.
- W4308808416 hasConcept C181199279 @default.
- W4308808416 hasConcept C185592680 @default.
- W4308808416 hasConcept C2776352461 @default.
- W4308808416 hasConcept C2777287306 @default.
- W4308808416 hasConcept C2777637488 @default.
- W4308808416 hasConcept C2779430813 @default.
- W4308808416 hasConcept C2779732960 @default.
- W4308808416 hasConcept C2780778831 @default.
- W4308808416 hasConcept C2781088250 @default.
- W4308808416 hasConcept C2781463711 @default.
- W4308808416 hasConcept C28212737 @default.
- W4308808416 hasConcept C43617362 @default.
- W4308808416 hasConcept C523546767 @default.
- W4308808416 hasConcept C54355233 @default.
- W4308808416 hasConcept C55493867 @default.
- W4308808416 hasConcept C59822182 @default.
- W4308808416 hasConcept C86803240 @default.
- W4308808416 hasConcept C89423630 @default.
- W4308808416 hasConceptScore W4308808416C101660505 @default.
- W4308808416 hasConceptScore W4308808416C116084860 @default.
- W4308808416 hasConceptScore W4308808416C181199279 @default.
- W4308808416 hasConceptScore W4308808416C185592680 @default.
- W4308808416 hasConceptScore W4308808416C2776352461 @default.
- W4308808416 hasConceptScore W4308808416C2777287306 @default.
- W4308808416 hasConceptScore W4308808416C2777637488 @default.