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- W4308844447 abstract "Abstract Biological pathways rely on the formation of intricate protein interaction networks called interactomes. Getting a comprehensive map of interactomes implies developing tools that allow capturing transient and low affinity protein-protein interactions (PPIs) in live conditions. Here we present an experimental strategy, Cell-PCA (Cell Protein Complementation Assay), which is based on BiFC (Bimolecular Fluorescence Complementation) and high throughput sequencing for ORFeome-wide analyses of different interactomes in the same live cell context. The specificity and sensitivity of Cell-PCA was established by using a wild type and a single amino-acid mutated HOXA9 protein, and the approach was subsequently applied for seven additional human HOX proteins. These proof-of-concept experiments revealed novel molecular properties of HOX interactomes and led to the identification of a novel cofactor of HOXB13 for promoting its proliferative activity in a cancer cell context. Taken together, our work demonstrates that Cell-PCA is pertinent for revealing and, importantly, comparing interactomes between different or highly related bait proteins in the same cell context." @default.
- W4308844447 created "2022-11-17" @default.
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- W4308844447 date "2022-11-09" @default.
- W4308844447 modified "2023-10-16" @default.
- W4308844447 title "A live cell protein complementation assay for ORFeome-wide probing of human HOX interactomes" @default.
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- W4308844447 doi "https://doi.org/10.1101/2022.11.09.515750" @default.
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