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• W4308889666 endingPage "102689" @default.
• W4308889666 startingPage "102689" @default.
• W4308889666 abstract "ATP-binding cassette (ABC) multidrug transporters are large, polytopic membrane proteins that exhibit astonishing promiscuity for their transport substrates. These transporters unidirectionally efflux thousands of structurally and functionally distinct compounds. To preclude the reentry of xenobiotic molecules via the drug-binding pocket, these proteins contain a highly conserved molecular gate, essentially allowing the transporters to function as molecular diodes. However, the structure-function relationship of these conserved gates and gating regions are not well characterized. In this study, we combine recent single-molecule, cryo-EM data with genetic and biochemical analyses of residues in the gating region of the yeast multidrug transporter Pdr5, the founding member of a large group of clinically relevant asymmetric ABC efflux pumps. Unlike the symmetric ABCG2 efflux gate, the Pdr5 counterpart is highly asymmetric, with only four (instead of six) residues comprising the gate proper. However, other residues in the near vicinity are essential for the gating activity. Furthermore, we demonstrate that residues in the gate and in the gating regions have multiple functions. For example, we show that Ile-685 and Val-1372 are required not only for successful efflux but also for allosteric inhibition of Pdr5 ATPase activity. Our investigations reveal that the gating region residues of Pdr5, and possibly other ABCG transporters, play a role not only in molecular gating but also in allosteric regulation, conformational switching, and protein folding." @default.
• W4308889666 created "2022-11-18" @default.
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• W4308889666 date "2022-12-01" @default.
• W4308889666 modified "2023-10-09" @default.
• W4308889666 title "Residues forming the gating regions of asymmetric multidrug transporter Pdr5 also play roles in conformational switching and protein folding" @default.
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• W4308889666 doi "https://doi.org/10.1016/j.jbc.2022.102689" @default.
• W4308889666 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36370844" @default.
• W4308889666 hasPublicationYear "2022" @default.
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