Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308951965> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W4308951965 endingPage "114649" @default.
- W4308951965 startingPage "114649" @default.
- W4308951965 abstract "Since 2016, the United States has experienced a resurgence in the number of hepatitis A virus (HAV) cases and outbreaks. These outbreaks have been sustained by person-to-person transmission with cases occurring predominantly in high-risk populations including intravenous drug users, individuals experiencing homelessness, and men who have sex with men. To investigate HAV transmission, a molecular-surveillance system consisting of real-time RT-PCR (rRT-PCR) for detection, and a conventional RT-PCR assay for genotyping of HAV, was established in New York State (NYS) in 2019. Since then, a total of 271 HAV-positive serum samples collected from cases across NYS between 2019 and 2021 were identified by rRT-PCR. To rapidly and efficiently generate HAV whole-genome sequences, a custom AmpliSeq™ panel was designed in collaboration with Thermo Fisher. To streamline the process, sample preparation was performed on an Ion Chef and sequencing on an Ion S5XL. Of the 271 HAV-positive samples, the whole-genome sequencing (WGS) assay successfully generated 134 near-complete, high-quality HAV sequences. Phylogenetic analysis of the VP1-2A region identified 216 IB, 48 IA, and 2 IIIA genotypes, while 5 were unable to be typed due to poor sequence in this key region. The HAV whole-genome sequencing approach provided a more efficient and streamlined approach for genotyping HAV compared to previous methods and resulted in phylogenetic trees with enhanced resolution compared to the HAV VP1-2A region alone." @default.
- W4308951965 created "2022-11-20" @default.
- W4308951965 creator A5028828764 @default.
- W4308951965 creator A5042037648 @default.
- W4308951965 creator A5043551928 @default.
- W4308951965 creator A5073898838 @default.
- W4308951965 creator A5076078321 @default.
- W4308951965 date "2023-02-01" @default.
- W4308951965 modified "2023-10-14" @default.
- W4308951965 title "A custom hepatitis A virus assay for whole-genome sequencing" @default.
- W4308951965 cites W2007381024 @default.
- W4308951965 cites W2125633654 @default.
- W4308951965 cites W2170776569 @default.
- W4308951965 cites W2753835363 @default.
- W4308951965 cites W2799524357 @default.
- W4308951965 cites W2944692805 @default.
- W4308951965 cites W2969114948 @default.
- W4308951965 cites W3162173707 @default.
- W4308951965 doi "https://doi.org/10.1016/j.jviromet.2022.114649" @default.
- W4308951965 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36375537" @default.
- W4308951965 hasPublicationYear "2023" @default.
- W4308951965 type Work @default.
- W4308951965 citedByCount "1" @default.
- W4308951965 countsByYear W43089519652023 @default.
- W4308951965 crossrefType "journal-article" @default.
- W4308951965 hasAuthorship W4308951965A5028828764 @default.
- W4308951965 hasAuthorship W4308951965A5042037648 @default.
- W4308951965 hasAuthorship W4308951965A5043551928 @default.
- W4308951965 hasAuthorship W4308951965A5073898838 @default.
- W4308951965 hasAuthorship W4308951965A5076078321 @default.
- W4308951965 hasConcept C104317684 @default.
- W4308951965 hasConcept C116675565 @default.
- W4308951965 hasConcept C135763542 @default.
- W4308951965 hasConcept C141231307 @default.
- W4308951965 hasConcept C159047783 @default.
- W4308951965 hasConcept C164928082 @default.
- W4308951965 hasConcept C193252679 @default.
- W4308951965 hasConcept C24432333 @default.
- W4308951965 hasConcept C2522874641 @default.
- W4308951965 hasConcept C3019983190 @default.
- W4308951965 hasConcept C31467283 @default.
- W4308951965 hasConcept C51679486 @default.
- W4308951965 hasConcept C54355233 @default.
- W4308951965 hasConcept C86803240 @default.
- W4308951965 hasConceptScore W4308951965C104317684 @default.
- W4308951965 hasConceptScore W4308951965C116675565 @default.
- W4308951965 hasConceptScore W4308951965C135763542 @default.
- W4308951965 hasConceptScore W4308951965C141231307 @default.
- W4308951965 hasConceptScore W4308951965C159047783 @default.
- W4308951965 hasConceptScore W4308951965C164928082 @default.
- W4308951965 hasConceptScore W4308951965C193252679 @default.
- W4308951965 hasConceptScore W4308951965C24432333 @default.
- W4308951965 hasConceptScore W4308951965C2522874641 @default.
- W4308951965 hasConceptScore W4308951965C3019983190 @default.
- W4308951965 hasConceptScore W4308951965C31467283 @default.
- W4308951965 hasConceptScore W4308951965C51679486 @default.
- W4308951965 hasConceptScore W4308951965C54355233 @default.
- W4308951965 hasConceptScore W4308951965C86803240 @default.
- W4308951965 hasFunder F4320310555 @default.
- W4308951965 hasFunder F4320332162 @default.
- W4308951965 hasLocation W43089519651 @default.
- W4308951965 hasLocation W43089519652 @default.
- W4308951965 hasOpenAccess W4308951965 @default.
- W4308951965 hasPrimaryLocation W43089519651 @default.
- W4308951965 hasRelatedWork W2013148429 @default.
- W4308951965 hasRelatedWork W2022681386 @default.
- W4308951965 hasRelatedWork W2063332314 @default.
- W4308951965 hasRelatedWork W2080371213 @default.
- W4308951965 hasRelatedWork W2734730316 @default.
- W4308951965 hasRelatedWork W3115231564 @default.
- W4308951965 hasRelatedWork W3166426295 @default.
- W4308951965 hasRelatedWork W4210376319 @default.
- W4308951965 hasRelatedWork W4236978444 @default.
- W4308951965 hasRelatedWork W4308951965 @default.
- W4308951965 hasVolume "312" @default.
- W4308951965 isParatext "false" @default.
- W4308951965 isRetracted "false" @default.
- W4308951965 workType "article" @default.