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- W4308953897 abstract "Abstract Background and Objective Melatonin plays an important role in various beneficial functions, including promoting differentiation. However, effects on osteogenic differentiation, especially in human periodontal cells (hPDLCs), still remain inconclusive. Mitochondria are highly dynamic organelles that play an important role in various biological processes in cells, including energy metabolism and oxidative stress reaction. Furthermore, the translocase of the outer mitochondrial membrane 20 (TOM20) is responsible for recognizing and transporting precursor proteins. Thus, the objective of this study was to evaluate the functionality of melatonin on osteogenesis in human periodontal cells and to explore the involved mechanism of mitochondria. Methods The hPDLCs were extracted and identified by flow cytometry and multilineage differentiation. We divided hPDLCs into control group, osteogenic induction group, and osteogenesis with melatonin treatment group (100, 10, and 1 μM). Then we used a specific siRNA to achieve interference of TOM20. Alizarin red and Alkaline phosphatase staining and activity assays were performed to evaluate osteogenic differentiation. Osteogenesis‐related genes and proteins were measured by qPCR and western blot. Mitochondrial functions were tested using ATP, NAD+/NADH, JC‐1, and Seahorse Mito Stress Test kits. Finally, TOM20 and mitochondrial dynamics‐related molecules expression were also assessed by qPCR and western blot. Results Our results showed that melatonin‐treated hPDLCs had higher calcification and ALP activity as well as upregulated OCN and Runx2 expression at mRNA and protein levels, which was the most obvious in 1 μM melatonin‐treated group. Meanwhile, melatonin supplement elevated intracellular ATP production and mitochondrial membrane potential by increasing mitochondrial oxidative metabolism, hence causing a lower NAD + /NADH ratio. In addition, we also found that melatonin treatment raised TOM20 level and osteogenesis and mitochondrial functions were both suppressed after knocking down TOM20. Conclusion We found that melatonin promoted osteogenesis of hPDLCs and 1 μM melatonin had the most remarkable effect. Melatonin treatment can reinforce mitochondrial functions by upregulating TOM20." @default.
- W4308953897 created "2022-11-20" @default.
- W4308953897 creator A5002262984 @default.
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- W4308953897 date "2022-11-13" @default.
- W4308953897 modified "2023-10-16" @default.
- W4308953897 title "Melatonin promoted osteogenesis of human periodontal ligament cells by regulating mitochondrial functions through the translocase of the outer mitochondrial membrane 20" @default.
- W4308953897 cites W1545865644 @default.
- W4308953897 cites W1766726626 @default.
- W4308953897 cites W1810629227 @default.
- W4308953897 cites W1913002361 @default.
- W4308953897 cites W1956327946 @default.
- W4308953897 cites W1962181495 @default.
- W4308953897 cites W1968455366 @default.
- W4308953897 cites W1973895988 @default.
- W4308953897 cites W1993103335 @default.
- W4308953897 cites W2002198698 @default.
- W4308953897 cites W2005008900 @default.
- W4308953897 cites W2008856079 @default.
- W4308953897 cites W2012428372 @default.
- W4308953897 cites W2014796710 @default.
- W4308953897 cites W2017950273 @default.
- W4308953897 cites W2018542907 @default.
- W4308953897 cites W2031536699 @default.
- W4308953897 cites W2033231390 @default.
- W4308953897 cites W2033704954 @default.
- W4308953897 cites W2035219417 @default.
- W4308953897 cites W2035428418 @default.
- W4308953897 cites W2044356754 @default.
- W4308953897 cites W2044934033 @default.
- W4308953897 cites W2050343565 @default.
- W4308953897 cites W2058444593 @default.
- W4308953897 cites W2062501347 @default.
- W4308953897 cites W2073095545 @default.
- W4308953897 cites W2074039437 @default.
- W4308953897 cites W2077574127 @default.
- W4308953897 cites W2079100436 @default.
- W4308953897 cites W2080659100 @default.
- W4308953897 cites W2101575450 @default.
- W4308953897 cites W2105786482 @default.
- W4308953897 cites W2131651601 @default.
- W4308953897 cites W2137158408 @default.
- W4308953897 cites W2138357624 @default.
- W4308953897 cites W2138736783 @default.
- W4308953897 cites W2144224215 @default.
- W4308953897 cites W2155643523 @default.
- W4308953897 cites W2165213062 @default.
- W4308953897 cites W2182559934 @default.
- W4308953897 cites W2223882087 @default.
- W4308953897 cites W2235239233 @default.
- W4308953897 cites W2266619630 @default.
- W4308953897 cites W2290909023 @default.
- W4308953897 cites W2319675468 @default.
- W4308953897 cites W2345442422 @default.
- W4308953897 cites W2470437549 @default.
- W4308953897 cites W2516887311 @default.
- W4308953897 cites W2563008150 @default.
- W4308953897 cites W2592262247 @default.
- W4308953897 cites W2725129410 @default.
- W4308953897 cites W2790831776 @default.
- W4308953897 cites W2803185543 @default.
- W4308953897 cites W2805970368 @default.
- W4308953897 cites W2889380083 @default.
- W4308953897 cites W2889602080 @default.
- W4308953897 cites W2898666867 @default.
- W4308953897 cites W2900479509 @default.
- W4308953897 cites W2910068913 @default.
- W4308953897 cites W2955145478 @default.
- W4308953897 cites W2967809789 @default.
- W4308953897 cites W3008596826 @default.
- W4308953897 cites W3010535956 @default.
- W4308953897 cites W3023626085 @default.
- W4308953897 cites W3035394348 @default.
- W4308953897 cites W3047248654 @default.
- W4308953897 cites W3087307234 @default.
- W4308953897 cites W3092537346 @default.
- W4308953897 cites W3116224215 @default.
- W4308953897 cites W3134917369 @default.
- W4308953897 cites W3158777904 @default.
- W4308953897 cites W3165578448 @default.
- W4308953897 cites W3181225632 @default.
- W4308953897 cites W3183028902 @default.
- W4308953897 cites W3191050852 @default.
- W4308953897 cites W4230233764 @default.
- W4308953897 cites W565439874 @default.
- W4308953897 doi "https://doi.org/10.1111/jre.13068" @default.
- W4308953897 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36373245" @default.
- W4308953897 hasPublicationYear "2022" @default.
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