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• W4308974117 abstract "Caseous lymphadenitis (CLA) is a disease that affects small ruminants, and the best way to prevent its spread on a herd is through immunoprophylaxis. Thus, we aimed to evaluate the MBP:PLD:CP40 fusion protein as a new CLA immunogen. The fusion protein was constructed by combining Corynebacterium pseudotuberculosis PLD and CP40 proteins with maltose-binding protein (MBP) as an intrinsic adjuvant. The antigenicity, allergenic potential, prediction of B epitopes, binding to MHC receptors, and docking on the Toll-Like 2 receptor were evaluated in silico. MBP:PLD:CP40 was expressed and purified. 40 BALB/c were divided into four groups (G1 - control, G2 - Saponin, G3 - MBP:PLD:CP40, and G4 - rPLD + rCP40). Total IgG, IgG1, and IgG2a were quantified, and the expressions of cytokines after splenocyte in vitro stimulation were assessed. Mice were challenged 42 days after the first immunization. The in silico analysis showed that MBP:PLD:CP40 has immunogenic potential, does not have allergic properties, and can dock on the TRL2 receptor. MBP:PLD:CP40 stimulated the production of IgG1 antibodies in a fivefold proportion to IgG2a, and TNF and IL-17 were significantly expressed in response to the antigenic stimuli. When rPLD and rCP40 were used together for immunization, they could induce IFN-γ and IL-12, but with no detectable antibody production. The G3 and G4 groups presented a survival of 57.14% and 42.86%, respectively, while the G1 and G2 mice were all dead 15 days after the challenge. MBP:PLD:CP40 partially protected the mice against C. pseudotuberculosis infection and can be considered a potential new CLA immunogen. KEY POINTS: • The fusion protein induced more IgG1 than IgG2a antibodies; • The fusion protein also induced the expression of the TNF and IL-17 cytokines; • Mice inoculated with MBP:PLD:CP40 presented a 57.14% survival." @default.
• W4308974117 created "2022-11-20" @default.
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• W4308974117 date "2022-11-14" @default.
• W4308974117 modified "2023-10-18" @default.
• W4308974117 title "Immunoprophylactic properties of the Corynebacterium pseudotuberculosis-derived MBP:PLD:CP40 fusion protein" @default.
• W4308974117 cites W1198449314 @default.
• W4308974117 cites W1526839960 @default.
• W4308974117 cites W1968095991 @default.
• W4308974117 cites W1970420451 @default.
• W4308974117 cites W1973343937 @default.
• W4308974117 cites W1986191025 @default.
• W4308974117 cites W1988642109 @default.
• W4308974117 cites W1990595793 @default.
• W4308974117 cites W1993568862 @default.
• W4308974117 cites W1998824336 @default.
• W4308974117 cites W2005707709 @default.
• W4308974117 cites W2009549360 @default.
• W4308974117 cites W2013934676 @default.
• W4308974117 cites W2022412760 @default.
• W4308974117 cites W2024018408 @default.
• W4308974117 cites W2024692796 @default.
• W4308974117 cites W2035671081 @default.
• W4308974117 cites W2041672734 @default.
• W4308974117 cites W2046452372 @default.
• W4308974117 cites W2050975649 @default.
• W4308974117 cites W2072765280 @default.
• W4308974117 cites W2078281797 @default.
• W4308974117 cites W2081912151 @default.
• W4308974117 cites W2084735562 @default.
• W4308974117 cites W2087530034 @default.
• W4308974117 cites W2088080494 @default.
• W4308974117 cites W2096495474 @default.
• W4308974117 cites W2102023232 @default.
• W4308974117 cites W2109971798 @default.
• W4308974117 cites W2110730271 @default.
• W4308974117 cites W2122779464 @default.
• W4308974117 cites W2136261088 @default.
• W4308974117 cites W2136918142 @default.
• W4308974117 cites W2140522686 @default.
• W4308974117 cites W2144021590 @default.
• W4308974117 cites W2163175900 @default.
• W4308974117 cites W2299334471 @default.
• W4308974117 cites W2340707163 @default.
• W4308974117 cites W2537469455 @default.
• W4308974117 cites W2738076021 @default.
• W4308974117 cites W2767444993 @default.
• W4308974117 cites W2770970215 @default.
• W4308974117 cites W2803393074 @default.
• W4308974117 cites W2947960138 @default.
• W4308974117 cites W2957776127 @default.
• W4308974117 cites W2963439065 @default.
• W4308974117 cites W3007680806 @default.
• W4308974117 cites W3014888375 @default.
• W4308974117 cites W3019619649 @default.
• W4308974117 cites W3024570138 @default.
• W4308974117 cites W3043036727 @default.
• W4308974117 cites W3102462840 @default.
• W4308974117 cites W3122352526 @default.
• W4308974117 cites W3129982058 @default.
• W4308974117 cites W3134432842 @default.
• W4308974117 cites W3134986627 @default.
• W4308974117 cites W3147929943 @default.
• W4308974117 cites W3191871285 @default.
• W4308974117 cites W3206959660 @default.
• W4308974117 cites W3216225891 @default.
• W4308974117 cites W4200285860 @default.
• W4308974117 cites W4210613731 @default.
• W4308974117 cites W4224621007 @default.
• W4308974117 cites W4244736178 @default.
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• W4308974117 doi "https://doi.org/10.1007/s00253-022-12279-1" @default.
• W4308974117 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36374330" @default.
• W4308974117 hasPublicationYear "2022" @default.
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