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- W4308976656 abstract "Abstract OBJECTIVE Translocator protein (TSPO) PET imaging is used in different neurological diseases and emerges as new tool for the diagnosis and therapy planning of glioma patients. Various signal sources of the TSPO-PET signal, however, hamper image interpretation and biological understanding. Here we assess the TSPO expression and ligand uptake in a murine model and human tissue derived from glioma patients. METHODS Mice with implanted GFP+ glioblastoma (n=20) or after sham implantation (n=14) were injected with the TSPO tracer [18F]GE-180 and received PET imaging. Tumors were harvested and processed to single cell suspension. Subsequently, tumors were either stained for TSPO, CD11b, ACSA2, GFAP and analyzed by flowcytometry or else magnetic cell sorting was performed to isolate tumor and CD11b+ cells that were measured by gamma counter. Multiple regression was used to determine contributions of GFP, CD11b, ACSA2 and GFAP positive cells to the total uptake. In TSPO PET-scans of glioma patients, regions with different GE-180 uptake were defined. Tumor samples (n=12) of these predefined areas were collected during surgery and analyzed by FACS as described above. RESULTS The expression of TSPO and PET signal in mice was increased in the tumor compared to tumor-free brain (SUVRGBM: 2.06 / SUVRSHAM: 0.92). FACS detected TSPO in tumor cells, microglia/macrophages and astrocytes. Tumor cells showed higher tracer uptake per cell when compared to CD11b+ cells (+31%). The contribution to the whole TSPO tracer uptake was high for tumor (β=0.41, P=0.018) and microglia/macrophages (β=0.35, P=0.042), and lowest for astrocytes (β=0.28, P=0.011). Patients with high-grade glioma indicated higher TSPO tracer uptake in GFAP+ cells when compared to CD11b+ cells and single cell uptake correlated with PET signal. CONCLUSION In experimental orthotopic glioblastoma, we could detect TSPO tracer signal at cellular resolution level. TSPO-PET can provide information about tumor and tumor-associated myeloid cells." @default.
- W4308976656 created "2022-11-20" @default.
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- W4308976656 date "2022-11-01" @default.
- W4308976656 modified "2023-09-28" @default.
- W4308976656 title "RBIO-05. QUANTIFICATION OF TSPO EXPRESSION AND TSPO RADIOLIGAND UPTAKE IN PET IN HUMAN AND MURINE GLIOBLASTOMA ON CELLULAR LEVEL" @default.
- W4308976656 doi "https://doi.org/10.1093/neuonc/noac209.957" @default.
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