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- W4308997614 abstract "Abstract Objective: To analyze the role of the metagenome next-generation sequencing (mNGS) technique in identifying and treating the pathogen of orthopedic chronic infectious diseases. Methods: The data of patients diagnosed with orthopedic chronic infectious diseases by a surgical team of the Department of Joint Surgery of the First Hospital of Jilin University from June to December 2021 were retrospectively analyzed. mNGS and pathogenic bacterial culture were performed on these patients, based on which, in combination with clinical manifestations and treatment effects, the role of mNGS in the diagnosis of pathogenic bacteria was determined. Results: All of the 12 patients had pain and swelling of the affected limb before hospitalization. Pathogenic bacteria were detected in eight patients by mNGS and in three patients by routine bacterial culture. 10 kinds of pathogenic bacteria ( Staphylococcus aureus , Streptococcus dysgalactiae , Pseudomonas stutzeri , Chaetomium globosum , Acinetobacter baumannii , Prevotella copri , Staphylococcus epidermidis , Sporothrix schenckii , Pseudomonas aeruginosa and Torque teno virus) were detected by mNGS. The same bacterium was detected in two cases by both mNGS and bacterial culture. Conclusion: The mNGS technique provides an important diagnostic basis for pathogen detection in orthopedic chronic infectious diseases." @default.
- W4308997614 created "2022-11-20" @default.
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- W4308997614 date "2022-11-14" @default.
- W4308997614 modified "2023-10-16" @default.
- W4308997614 title "Metagenome Next-Generation Sequencing Technique in Diagnosis of Orthopedic Chronic Infectious Diseases (A Report of 12 Cases)" @default.
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- W4308997614 doi "https://doi.org/10.21203/rs.3.rs-2255832/v1" @default.
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