FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4309095895> ?p ?o ?g. }

• W4309095895 abstract "Abstract Small ubiquitin‐like modifiers (SUMOs) are conjugated to protein substrates in cells to regulate their function. The attachment of SUMO family members SUMO1‐3 to substrate proteins is reversed by specific isopeptidases called SENPs (sentrin‐specific protease). Whereas SENPs are SUMO‐isoform or linkage type specific, comprehensive analysis is missing. Furthermore, the underlying mechanism of SENP linkage specificity remains unclear. We present a high‐throughput synthesis of 83 isopeptide‐linked SUMO‐based fluorescence polarization reagents to study enzyme preferences. The assay reagents were synthesized via a native chemical ligation‐desulfurization protocol between 11‐mer peptides containing a γ‐thiolysine and a SUMO3 thioester. Subsequently, five recombinantly expressed SENPs were screened using these assay reagents to reveal their deconjugation activity and substrate preferences. In general, we observed that SENP1 is the most active and nonselective SENP while SENP6 and SENP7 show the least activity. Furthermore, SENPs differentially process peptides derived from SUMO1‐3, who form a minimalistic representation of diSUMO chains. To validate our findings, five distinct isopeptide‐linked diSUMO chains were chemically synthesized and proteolysis was monitored using a gel‐based read‐out." @default.
• W4309095895 created "2022-11-21" @default.
• W4309095895 creator A5033653145 @default.
• W4309095895 creator A5043122653 @default.
• W4309095895 creator A5054213383 @default.
• W4309095895 creator A5057076424 @default.
• W4309095895 creator A5060430222 @default.
• W4309095895 creator A5088622927 @default.
• W4309095895 creator A5091557223 @default.
• W4309095895 date "2022-12-07" @default.
• W4309095895 modified "2023-09-30" @default.
• W4309095895 title "In‐Plate Chemical Synthesis of Isopeptide‐Linked SUMOylated Peptide Fluorescence Polarization Reagents for High‐Throughput Screening of SENP Preferences" @default.
• W4309095895 cites W1520109974 @default.
• W4309095895 cites W1892720553 @default.
• W4309095895 cites W1983606049 @default.
• W4309095895 cites W1993287528 @default.
• W4309095895 cites W2001041080 @default.
• W4309095895 cites W2013108773 @default.
• W4309095895 cites W2019437977 @default.
• W4309095895 cites W2032997281 @default.
• W4309095895 cites W2037845650 @default.
• W4309095895 cites W2046477151 @default.
• W4309095895 cites W2048917471 @default.
• W4309095895 cites W2071545806 @default.
• W4309095895 cites W2082276184 @default.
• W4309095895 cites W2096569452 @default.
• W4309095895 cites W2110768070 @default.
• W4309095895 cites W2116179149 @default.
• W4309095895 cites W2119415370 @default.
• W4309095895 cites W2121327477 @default.
• W4309095895 cites W2125074509 @default.
• W4309095895 cites W2152630268 @default.
• W4309095895 cites W2167536088 @default.
• W4309095895 cites W2326360051 @default.
• W4309095895 cites W2402259557 @default.
• W4309095895 cites W2490844245 @default.
• W4309095895 cites W2528900269 @default.
• W4309095895 cites W2769933221 @default.
• W4309095895 cites W2791163698 @default.
• W4309095895 cites W2793014640 @default.
• W4309095895 cites W2890264162 @default.
• W4309095895 cites W2896017147 @default.
• W4309095895 cites W2922950467 @default.
• W4309095895 cites W2966458893 @default.
• W4309095895 cites W2971643529 @default.
• W4309095895 cites W2973726073 @default.
• W4309095895 cites W2989432634 @default.
• W4309095895 cites W3014809609 @default.
• W4309095895 cites W3089452157 @default.
• W4309095895 cites W4200587905 @default.
• W4309095895 cites W4229628765 @default.
• W4309095895 cites W4283377063 @default.
• W4309095895 cites W4309095895 @default.
• W4309095895 cites W4381153445 @default.
• W4309095895 cites W95252060 @default.
• W4309095895 doi "https://doi.org/10.1002/cbic.202200601" @default.
• W4309095895 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36377600" @default.
• W4309095895 hasPublicationYear "2022" @default.
• W4309095895 type Work @default.
• W4309095895 citedByCount "1" @default.
• W4309095895 countsByYear W43090958952022 @default.
• W4309095895 crossrefType "journal-article" @default.
• W4309095895 hasAuthorship W4309095895A5033653145 @default.
• W4309095895 hasAuthorship W4309095895A5043122653 @default.
• W4309095895 hasAuthorship W4309095895A5054213383 @default.
• W4309095895 hasAuthorship W4309095895A5057076424 @default.
• W4309095895 hasAuthorship W4309095895A5060430222 @default.
• W4309095895 hasAuthorship W4309095895A5088622927 @default.
• W4309095895 hasAuthorship W4309095895A5091557223 @default.
• W4309095895 hasBestOaLocation W43090958951 @default.
• W4309095895 hasConcept C104317684 @default.
• W4309095895 hasConcept C147789679 @default.
• W4309095895 hasConcept C171350616 @default.
• W4309095895 hasConcept C181199279 @default.
• W4309095895 hasConcept C185592680 @default.
• W4309095895 hasConcept C25602115 @default.
• W4309095895 hasConcept C2779281246 @default.
• W4309095895 hasConcept C40875361 @default.
• W4309095895 hasConcept C55493867 @default.
• W4309095895 hasConcept C86803240 @default.
• W4309095895 hasConceptScore W4309095895C104317684 @default.
• W4309095895 hasConceptScore W4309095895C147789679 @default.
• W4309095895 hasConceptScore W4309095895C171350616 @default.
• W4309095895 hasConceptScore W4309095895C181199279 @default.
• W4309095895 hasConceptScore W4309095895C185592680 @default.
• W4309095895 hasConceptScore W4309095895C25602115 @default.
• W4309095895 hasConceptScore W4309095895C2779281246 @default.
• W4309095895 hasConceptScore W4309095895C40875361 @default.
• W4309095895 hasConceptScore W4309095895C55493867 @default.
• W4309095895 hasConceptScore W4309095895C86803240 @default.
• W4309095895 hasIssue "2" @default.
• W4309095895 hasLocation W43090958951 @default.
• W4309095895 hasLocation W43090958952 @default.
• W4309095895 hasLocation W43090958953 @default.
• W4309095895 hasLocation W43090958954 @default.
• W4309095895 hasOpenAccess W4309095895 @default.
• W4309095895 hasPrimaryLocation W43090958951 @default.
• W4309095895 hasRelatedWork W1970587968 @default.
• W4309095895 hasRelatedWork W1971187521 @default.
• W4309095895 hasRelatedWork W2018201204 @default.

• next