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• W4309103019 abstract "Abstract We present direct-LIVE-PAINT, an easy-to-implement approach for the nanoscopic imaging of protein structures in live cells using labeled binding peptides. We demonstrate the feasibility of direct-LIVE-PAINT with an actin-binding peptide fused to EGFP, the location of which can be accurately determined as it transiently binds to actin filaments. We show that direct-LIVE-PAINT can be used to image actin structures below the diffraction-limit of light and have used it to observe the dynamic nature of actin in live cells. We envisage a similar approach could be applied to imaging other proteins within live mammalian cells." @default.
• W4309103019 created "2022-11-22" @default.
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• W4309103019 date "2022-11-15" @default.
• W4309103019 modified "2023-09-29" @default.
• W4309103019 title "Live-cell super-resolution imaging of actin using LifeAct-14 with a PAINT-based approach" @default.
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• W4309103019 doi "https://doi.org/10.1101/2022.11.15.516635" @default.
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