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- W4309125752 abstract "The goat specific nucleotide sequence for HSP70 gene will be retrieved from NCBI public database. Gradient PCR will be carried out to optimize the annealing temperature for HSP70 genes using the thermoycler. The optimum concentration of PCR reaction components will be standardized. The genomic DNA isolated from the blood samples will be checked for quality, purity and concentration. Only the DNA samples of good quality, purity and concentration will be used for further analysis. Horizontal submarine agarose gel electrophoresis will be performed to check the quality of genomic DNA using 0.8 % agarose. The purity of genomic DNA will be checked using UV-Spectrophotometer. The agarose solution will be poured in to the sealed casting tray. The gel will be prepared to about 4 mm thicknesses. The agarose gel will be allowed to set completely at 4°C temperature and then the comb will be gently removed." @default.
- W4309125752 created "2022-11-23" @default.
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- W4309125752 date "2022-11-16" @default.
- W4309125752 modified "2023-09-25" @default.
- W4309125752 title "Identification of HSP-70 Gene in Ganjam Goat: DNA Extraction, PCR and Electrophoresis" @default.
- W4309125752 doi "https://doi.org/10.1201/9781003364689-25" @default.
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