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- W4309158616 abstract "Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels─horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)─UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection." @default.
- W4309158616 created "2022-11-24" @default.
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- W4309158616 date "2022-11-16" @default.
- W4309158616 modified "2023-10-07" @default.
- W4309158616 title "Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays" @default.
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- W4309158616 cites W2002384741 @default.
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- W4309158616 cites W2030652025 @default.
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- W4309158616 doi "https://doi.org/10.1021/acs.analchem.2c03543" @default.
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- W4309158616 hasPublicationYear "2022" @default.
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