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• W4309164579 endingPage "19" @default.
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• W4309164579 abstract "Abstract The GTPase FtsZ forms the cell division scaffold in bacteria, which mediates the recruitment of the other components of the divisome. Streptomycetes undergo two different forms of cell division. Septa without detectable peptidoglycan divide the highly compartmentalised young hyphae during early vegetative growth, and cross-walls are formed that dissect the hyphae into long multinucleoid compartments in the substrate mycelium, while ladders of septa are formed in the aerial hyphae that lead to chains of uninucleoid spores. In a previous study, we analysed the phosphoproteome of Streptomyces coelicolor and showed that FtsZ is phosphorylated at Ser 317 and Ser389. Substituting Ser–Ser for either Glu–Glu (mimicking phosphorylation) or Ala–Ala (mimicking non-phosphorylation) hinted at changes in antibiotic production. Here we analyse development, colony morphology, spore resistance, and antibiotic production in FtsZ knockout mutants expressing FtsZ alleles mimicking Ser319 and Ser387 phosphorylation and non-phosphorylation: AA (no phosphorylation), AE, EA (mixed), and EE (double phosphorylation). The FtsZ-eGFP AE, EA and EE alleles were not able to form observable FtsZ-eGFP ladders when they were expressed in the S. coelicolor wild-type strain, whereas the AA allele could form apparently normal eGFP Z-ladders. The FtsZ mutant expressing the FtsZ EE or EA or AE alleles is able to sporulate indicating that the mutant alleles are able to form functional Z-rings leading to sporulation when the wild-type FtsZ gene is absent. The four mutants were pleiotropically affected in colony morphogenesis, antibiotic production, substrate mycelium differentiation and sporulation (sporulation timing and spore resistance) which may be an indirect result of the effect in sporulation Z-ladder formation. Each mutant showed a distinctive phenotype in antibiotic production, single colony morphology, and sporulation (sporulation timing and spore resistance) indicating that the different FtsZ phosphomimetic alleles led to different phenotypes. Taken together, our data provide evidence for a pleiotropic effect of FtsZ phosphorylation in colony morphology, antibiotic production, and sporulation." @default.
• W4309164579 created "2022-11-24" @default.
• W4309164579 creator A5034011155 @default.
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• W4309164579 creator A5051793026 @default.
• W4309164579 creator A5063691411 @default.
• W4309164579 creator A5091052284 @default.
• W4309164579 date "2022-11-16" @default.
• W4309164579 modified "2023-10-05" @default.
• W4309164579 title "FtsZ phosphorylation pleiotropically affects Z-ladder formation, antibiotic production, and morphogenesis in Streptomyces coelicolor" @default.
• W4309164579 cites W1482784312 @default.
• W4309164579 cites W1571701196 @default.
• W4309164579 cites W1651953691 @default.
• W4309164579 cites W1963836821 @default.
• W4309164579 cites W1981562747 @default.
• W4309164579 cites W1990652761 @default.
• W4309164579 cites W1990762571 @default.
• W4309164579 cites W1993120768 @default.
• W4309164579 cites W2004480958 @default.
• W4309164579 cites W2009156161 @default.
• W4309164579 cites W2019109040 @default.
• W4309164579 cites W2025109401 @default.
• W4309164579 cites W2025168003 @default.
• W4309164579 cites W2030687678 @default.
• W4309164579 cites W2031429120 @default.
• W4309164579 cites W2034672527 @default.
• W4309164579 cites W2035108970 @default.
• W4309164579 cites W2039616785 @default.
• W4309164579 cites W2047712032 @default.
• W4309164579 cites W2055208759 @default.
• W4309164579 cites W2056354361 @default.
• W4309164579 cites W2059397761 @default.
• W4309164579 cites W2064477837 @default.
• W4309164579 cites W2064910808 @default.
• W4309164579 cites W2065125816 @default.
• W4309164579 cites W2068603795 @default.
• W4309164579 cites W2070067221 @default.
• W4309164579 cites W2073210733 @default.
• W4309164579 cites W2074374487 @default.
• W4309164579 cites W2080696275 @default.
• W4309164579 cites W2086506761 @default.
• W4309164579 cites W2087463186 @default.
• W4309164579 cites W2087675721 @default.
• W4309164579 cites W2090760876 @default.
• W4309164579 cites W2097255063 @default.
• W4309164579 cites W2099704861 @default.
• W4309164579 cites W2113818747 @default.
• W4309164579 cites W2114427530 @default.
• W4309164579 cites W2120762679 @default.
• W4309164579 cites W2122956742 @default.
• W4309164579 cites W2123175793 @default.
• W4309164579 cites W2125328556 @default.
• W4309164579 cites W2127359880 @default.
• W4309164579 cites W2128895464 @default.
• W4309164579 cites W2133942178 @default.
• W4309164579 cites W2134223473 @default.
• W4309164579 cites W2139206963 @default.
• W4309164579 cites W2142314320 @default.
• W4309164579 cites W2142779706 @default.
• W4309164579 cites W2144636962 @default.
• W4309164579 cites W2145981596 @default.
• W4309164579 cites W2149248785 @default.
• W4309164579 cites W2153616142 @default.
• W4309164579 cites W2155658342 @default.
• W4309164579 cites W2155967620 @default.
• W4309164579 cites W2161580505 @default.
• W4309164579 cites W2167279371 @default.
• W4309164579 cites W2171555414 @default.
• W4309164579 cites W2178324140 @default.
• W4309164579 cites W2224175912 @default.
• W4309164579 cites W2235714155 @default.
• W4309164579 cites W2240400464 @default.
• W4309164579 cites W2294146745 @default.
• W4309164579 cites W2313346828 @default.
• W4309164579 cites W2317735273 @default.
• W4309164579 cites W2334805793 @default.
• W4309164579 cites W2340796147 @default.
• W4309164579 cites W2435566834 @default.
• W4309164579 cites W2518177070 @default.
• W4309164579 cites W2591612535 @default.
• W4309164579 cites W2606657672 @default.
• W4309164579 cites W2613570472 @default.
• W4309164579 cites W2613930464 @default.
• W4309164579 cites W2728813546 @default.
• W4309164579 cites W2766697512 @default.
• W4309164579 cites W2767390402 @default.
• W4309164579 cites W2791400855 @default.
• W4309164579 cites W2801535404 @default.
• W4309164579 cites W2802022017 @default.
• W4309164579 cites W2803591909 @default.
• W4309164579 cites W2883353049 @default.
• W4309164579 cites W2939410939 @default.
• W4309164579 cites W2945855511 @default.
• W4309164579 cites W2960027354 @default.
• W4309164579 cites W2985543675 @default.
• W4309164579 cites W2988612253 @default.
• W4309164579 cites W2999348879 @default.

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