Matches in SemOpenAlex for { <https://semopenalex.org/work/W4309197404> ?p ?o ?g. }
- W4309197404 endingPage "489" @default.
- W4309197404 startingPage "477" @default.
- W4309197404 abstract "The subclass Coccidia comprises a large group of protozoan parasites, including important pathogens of humans and animals such as Toxoplasma gondii, Neospora caninum, Eimeria spp., and Cystoisospora spp. Their life cycle includes a switch from asexual to sexual stages and is often restricted to a single host species. Current research on coccidian parasites focuses on cell biology and the underlying mechanisms of protein expression and trafficking in different life stages, host cell invasion and host-parasite interactions. Furthermore, novel anticoccidial drug targets are evaluated. Given the variety of research questions and the requirement to reduce and replace animal experimentation, in vitro cultivation of Coccidia needs to be further developed and refined to meet these requirements. For these purposes, established culture systems are constantly improved. In addition, new in vitro culture systems lately gained considerable importance in research on Coccidia. Well established and optimized in vitro cultures of monolayer cells can support the viability and development of parasite stages and even allow completion of the life cycle in vitro, as shown for Cystoisospora suis and Eimeria tenella. Furthermore, new three-dimensional cell culture models are used for propagation of Cryptosporidium spp. (close relatives of the coccidians), and the infection of three-dimensional organoids with T. gondii also gained popularity as the interaction between the parasite and host tissue can be studied in more detail. The latest advances in three-dimensional culture systems are organ-on-a-chip models, that to date have only been tested for T. gondii but promise to accelerate research in other coccidians. Lastly, the completion of the life cycle of C. suis and Cryptosporidium parvum was reported to continue in a host cell-free environment following the first occurrence of asexual stages. Such axenic cultures are becoming increasingly available and open new avenues for research on parasite life cycle stages and novel intervention strategies." @default.
- W4309197404 created "2022-11-24" @default.
- W4309197404 creator A5021579176 @default.
- W4309197404 creator A5079868810 @default.
- W4309197404 creator A5087434388 @default.
- W4309197404 creator A5088570452 @default.
- W4309197404 date "2023-08-01" @default.
- W4309197404 modified "2023-10-14" @default.
- W4309197404 title "In vitro cultivation methods for coccidian parasite research" @default.
- W4309197404 cites W10443405 @default.
- W4309197404 cites W1524658934 @default.
- W4309197404 cites W1756115834 @default.
- W4309197404 cites W1760381734 @default.
- W4309197404 cites W1793558192 @default.
- W4309197404 cites W1938836952 @default.
- W4309197404 cites W1952642745 @default.
- W4309197404 cites W1963571697 @default.
- W4309197404 cites W1965157206 @default.
- W4309197404 cites W1966008931 @default.
- W4309197404 cites W1966123374 @default.
- W4309197404 cites W1969031921 @default.
- W4309197404 cites W1972192292 @default.
- W4309197404 cites W1972461452 @default.
- W4309197404 cites W1972558516 @default.
- W4309197404 cites W1973653794 @default.
- W4309197404 cites W1976343742 @default.
- W4309197404 cites W1984706770 @default.
- W4309197404 cites W1987518328 @default.
- W4309197404 cites W1991179070 @default.
- W4309197404 cites W1994087706 @default.
- W4309197404 cites W1997302071 @default.
- W4309197404 cites W2000664703 @default.
- W4309197404 cites W2001279976 @default.
- W4309197404 cites W2002328188 @default.
- W4309197404 cites W2006634312 @default.
- W4309197404 cites W2008622471 @default.
- W4309197404 cites W2011944521 @default.
- W4309197404 cites W2011977647 @default.
- W4309197404 cites W2013392648 @default.
- W4309197404 cites W2013878437 @default.
- W4309197404 cites W2016164331 @default.
- W4309197404 cites W2018453465 @default.
- W4309197404 cites W2019314802 @default.
- W4309197404 cites W2021207689 @default.
- W4309197404 cites W2023902486 @default.
- W4309197404 cites W2025780168 @default.
- W4309197404 cites W2026930884 @default.
- W4309197404 cites W2029746400 @default.
- W4309197404 cites W2030717623 @default.
- W4309197404 cites W2034494213 @default.
- W4309197404 cites W2036456050 @default.
- W4309197404 cites W2038136953 @default.
- W4309197404 cites W2039653942 @default.
- W4309197404 cites W2040100208 @default.
- W4309197404 cites W2040333940 @default.
- W4309197404 cites W2041422954 @default.
- W4309197404 cites W2041727540 @default.
- W4309197404 cites W2044023688 @default.
- W4309197404 cites W2046371898 @default.
- W4309197404 cites W2047483825 @default.
- W4309197404 cites W2049897034 @default.
- W4309197404 cites W2050969183 @default.
- W4309197404 cites W2051403859 @default.
- W4309197404 cites W2052244601 @default.
- W4309197404 cites W2052690976 @default.
- W4309197404 cites W2054530737 @default.
- W4309197404 cites W2059964587 @default.
- W4309197404 cites W2061223068 @default.
- W4309197404 cites W2061786996 @default.
- W4309197404 cites W2062151827 @default.
- W4309197404 cites W2062165684 @default.
- W4309197404 cites W2062875077 @default.
- W4309197404 cites W2063472355 @default.
- W4309197404 cites W2065000386 @default.
- W4309197404 cites W2065846024 @default.
- W4309197404 cites W2069903993 @default.
- W4309197404 cites W2072324351 @default.
- W4309197404 cites W2072437217 @default.
- W4309197404 cites W2073302338 @default.
- W4309197404 cites W2074539326 @default.
- W4309197404 cites W2075416945 @default.
- W4309197404 cites W2075634590 @default.
- W4309197404 cites W2080516804 @default.
- W4309197404 cites W2080636610 @default.
- W4309197404 cites W2083740480 @default.
- W4309197404 cites W2084281420 @default.
- W4309197404 cites W2085559150 @default.
- W4309197404 cites W2091365100 @default.
- W4309197404 cites W2099611549 @default.
- W4309197404 cites W2101840086 @default.
- W4309197404 cites W2102879924 @default.
- W4309197404 cites W2105431767 @default.
- W4309197404 cites W2110598936 @default.
- W4309197404 cites W2110839574 @default.
- W4309197404 cites W2113454677 @default.
- W4309197404 cites W2115269778 @default.
- W4309197404 cites W2117962690 @default.
- W4309197404 cites W2124140654 @default.