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- W4309202294 endingPage "17038" @default.
- W4309202294 startingPage "17029" @default.
- W4309202294 abstract "Oxidative stress mediated by reactive oxygen species (ROS) is a key process for adverse aerosol health effects. Secondary organic aerosols (SOA) account for a major fraction of fine particulate matter, and their inhalation and deposition into the respiratory tract causes the formation of ROS by chemical and cellular processes, but their relative contributions are hardly quantified and their link to oxidative stress remains uncertain. Here, we quantified cellular and chemical superoxide generation by 9,10-phenanthrenequinone (PQN) and isoprene SOA using a chemiluminescence assay combined with electron paramagnetic resonance spectroscopy as well as kinetic modeling. We also applied cellular imaging techniques to study the cellular mechanism of superoxide release and oxidative damage on cell membranes. We show that PQN and isoprene SOA activate NADPH oxidase in macrophages to release massive amounts of superoxide, overwhelming the superoxide formation by aqueous chemical reactions in the epithelial lining fluid. The activation dose for PQN is 2 orders of magnitude lower than that of isoprene SOA, suggesting that quinones are more toxic. While higher exposures trigger cellular antioxidant response elements, the released ROS induce oxidative damage to the cell membrane through lipid peroxidation. Such mechanistic and quantitative understandings provide a basis for further elucidation of adverse health effects and oxidative stress by fine particulate matter." @default.
- W4309202294 created "2022-11-24" @default.
- W4309202294 creator A5005353316 @default.
- W4309202294 creator A5020976244 @default.
- W4309202294 creator A5029798816 @default.
- W4309202294 creator A5068502346 @default.
- W4309202294 creator A5070221280 @default.
- W4309202294 creator A5070324997 @default.
- W4309202294 creator A5084154416 @default.
- W4309202294 creator A5088747461 @default.
- W4309202294 date "2022-11-17" @default.
- W4309202294 modified "2023-10-14" @default.
- W4309202294 title "Superoxide Release by Macrophages through NADPH Oxidase Activation Dominating Chemistry by Isoprene Secondary Organic Aerosols and Quinones to Cause Oxidative Damage on Membranes" @default.
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