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- W4309288482 abstract "Radiation resistance represents a major challenge in the treatment of breast cancer. As heparan sulfate (HS) chains are known to contribute to tumorigenesis, we aimed to investigate the interplay between HS degradation and radiation response in triple-negative breast cancer (TNBC) cells.HS chains were degraded in vitro as TNBC cells MDA-MB-231 and HCC1806 were treated with heparinase I and III. Subsequently, radioresistance was determined via colony formation assay after doses of 2, 4 and 6 Gy. Cell cycle profile, stem cell characteristics, expression of HS, activation of beta integrins, and apoptosis were determined by flow cytometry. Additionally, cell motility was analyzed via wound-healing assays, and expression and activation of FAK, CDK-6, Src, and Erk1/2 were quantified by western blot pre- and post-irradiation. Finally, the expression of cytokines was analyzed using a cytokine array.Radiation promoted cell cycle changes, while heparinase treatment induced apoptosis in both cell lines. Colony formation assays showed significantly increased radio-resistance for both cell lines after degradation of HS. Cell migration was similarly upregulated after degradation of HS compared to controls. This effect was even more prominent after irradiation. Interestingly, FAK, a marker of radioresistance, was significantly activated in the heparinase-treated group. Additionally, we found Src to be dysregulated in MDA-MB-231 cells. Finally, we observed differential secretion of GRO, CXCL1, IGFBP1, IL8, Angiogenin, and Osteoprotegerin after HS degradation and radiotherapy.Our results suggest an influence of HS chains on the development of radioresistance in TNBC." @default.
- W4309288482 created "2022-11-25" @default.
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- W4309288482 date "2022-12-01" @default.
- W4309288482 modified "2023-09-27" @default.
- W4309288482 title "Enzymatic Digestion of Cell-surface Heparan Sulfate Alters the Radiation Response in Triple-negative Breast Cancer Cells" @default.
- W4309288482 cites W1522174324 @default.
- W4309288482 cites W1582656672 @default.
- W4309288482 cites W1967997862 @default.
- W4309288482 cites W1971073715 @default.
- W4309288482 cites W1974207755 @default.
- W4309288482 cites W1975796762 @default.
- W4309288482 cites W1977549713 @default.
- W4309288482 cites W1981444673 @default.
- W4309288482 cites W1982819887 @default.
- W4309288482 cites W1993311913 @default.
- W4309288482 cites W1997613697 @default.
- W4309288482 cites W2004240996 @default.
- W4309288482 cites W2006323138 @default.
- W4309288482 cites W2009962397 @default.
- W4309288482 cites W2012339228 @default.
- W4309288482 cites W2013232219 @default.
- W4309288482 cites W2017229935 @default.
- W4309288482 cites W2018242510 @default.
- W4309288482 cites W2044126187 @default.
- W4309288482 cites W2047533646 @default.
- W4309288482 cites W2048467313 @default.
- W4309288482 cites W2050225501 @default.
- W4309288482 cites W2068316985 @default.
- W4309288482 cites W2076788883 @default.
- W4309288482 cites W2077927052 @default.
- W4309288482 cites W2080974514 @default.
- W4309288482 cites W2094001485 @default.
- W4309288482 cites W2095020934 @default.
- W4309288482 cites W2096629111 @default.
- W4309288482 cites W2096942445 @default.
- W4309288482 cites W2100025800 @default.
- W4309288482 cites W2100959766 @default.
- W4309288482 cites W2112654580 @default.
- W4309288482 cites W2117692326 @default.
- W4309288482 cites W2121411022 @default.
- W4309288482 cites W2123169639 @default.
- W4309288482 cites W2130280698 @default.
- W4309288482 cites W2134525336 @default.
- W4309288482 cites W2147617210 @default.
- W4309288482 cites W2150250127 @default.
- W4309288482 cites W2164071805 @default.
- W4309288482 cites W2170602872 @default.
- W4309288482 cites W2265789527 @default.
- W4309288482 cites W2399474824 @default.
- W4309288482 cites W2528705363 @default.
- W4309288482 cites W2557183800 @default.
- W4309288482 cites W2734542648 @default.
- W4309288482 cites W2767624974 @default.
- W4309288482 cites W2771119952 @default.
- W4309288482 cites W2810651251 @default.
- W4309288482 cites W2888294728 @default.
- W4309288482 cites W2890624098 @default.
- W4309288482 cites W2900367378 @default.
- W4309288482 cites W2916628225 @default.
- W4309288482 cites W2949922878 @default.
- W4309288482 cites W2952051913 @default.
- W4309288482 cites W2962706882 @default.
- W4309288482 cites W2970599526 @default.
- W4309288482 cites W3002317446 @default.
- W4309288482 cites W3006709689 @default.
- W4309288482 cites W3010659583 @default.
- W4309288482 cites W3013394636 @default.
- W4309288482 cites W3014731966 @default.
- W4309288482 cites W3021216386 @default.
- W4309288482 cites W3021955061 @default.
- W4309288482 cites W3025501970 @default.
- W4309288482 cites W3033869790 @default.
- W4309288482 cites W3037436934 @default.
- W4309288482 cites W3084054647 @default.
- W4309288482 cites W3094903561 @default.
- W4309288482 cites W3103176672 @default.
- W4309288482 cites W3108991717 @default.
- W4309288482 cites W3121918553 @default.
- W4309288482 cites W3147142721 @default.
- W4309288482 cites W3153164029 @default.
- W4309288482 cites W3180251219 @default.
- W4309288482 cites W3193761565 @default.
- W4309288482 cites W3216462497 @default.
- W4309288482 cites W4213043006 @default.
- W4309288482 cites W4226165381 @default.
- W4309288482 cites W4280609709 @default.
- W4309288482 doi "https://doi.org/10.1016/j.arcmed.2022.11.004" @default.
- W4309288482 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36411172" @default.
- W4309288482 hasPublicationYear "2022" @default.
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