Matches in SemOpenAlex for { <https://semopenalex.org/work/W4309297112> ?p ?o ?g. }
- W4309297112 abstract "Cardiovascular disease is the leading cause of death worldwide due to the inability of adult heart to regenerate after injury. N 6 -methyladenosine (m 6 A) methylation catalyzed by the enzyme methyltransferase-like 3 (Mettl3) plays an important role in various physiological and pathological bioprocesses. However, the role of m 6 A in heart regeneration remains largely unclear. To study m 6 A function in heart regeneration, we modulated Mettl3 expression in vitro and in vivo. Knockdown of Mettl3 significantly increased the proliferation of cardiomyocytes and accelerated heart regeneration following heart injury in neonatal and adult mice. However, Mettl3 overexpression decreased cardiomyocyte proliferation and suppressed heart regeneration in postnatal mice. Conjoint analysis of methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-seq identified Fgf16 as a downstream target of Mettl3-mediated m 6 A modification during postnatal heart regeneration. RIP-qPCR and luciferase reporter assays revealed that Mettl3 negatively regulates Fgf16 mRNA expression in an m 6 A-Ythdf2-dependent manner. The silencing of Fgf16 suppressed the proliferation of cardiomyocytes. However, the overexpression of ΔFgf16, in which the m 6 A consensus sequence was mutated, significantly increased cardiomyocyte proliferation and accelerated heart regeneration in postnatal mice compared with wild-type Fgf16. Our data demonstrate that Mettl3 post-transcriptionally reduces Fgf16 mRNA levels through an m 6 A-Ythdf2-dependen pathway, thereby controlling cardiomyocyte proliferation and heart regeneration." @default.
- W4309297112 created "2022-11-25" @default.
- W4309297112 creator A5013054536 @default.
- W4309297112 creator A5013547596 @default.
- W4309297112 creator A5025372159 @default.
- W4309297112 creator A5026303073 @default.
- W4309297112 creator A5039339633 @default.
- W4309297112 creator A5042459440 @default.
- W4309297112 creator A5061273891 @default.
- W4309297112 creator A5064150326 @default.
- W4309297112 creator A5065102601 @default.
- W4309297112 creator A5068498943 @default.
- W4309297112 creator A5072437024 @default.
- W4309297112 creator A5077787768 @default.
- W4309297112 creator A5082471415 @default.
- W4309297112 creator A5085876096 @default.
- W4309297112 creator A5090666373 @default.
- W4309297112 creator A5090748128 @default.
- W4309297112 date "2022-11-18" @default.
- W4309297112 modified "2023-10-03" @default.
- W4309297112 title "Mettl3-mediated m6A modification of Fgf16 restricts cardiomyocyte proliferation during heart regeneration" @default.
- W4309297112 cites W1764846306 @default.
- W4309297112 cites W1941339056 @default.
- W4309297112 cites W1969038150 @default.
- W4309297112 cites W1969210272 @default.
- W4309297112 cites W1977430213 @default.
- W4309297112 cites W1988378146 @default.
- W4309297112 cites W1989021280 @default.
- W4309297112 cites W1991807334 @default.
- W4309297112 cites W1992644002 @default.
- W4309297112 cites W1999574084 @default.
- W4309297112 cites W2002458471 @default.
- W4309297112 cites W2007429249 @default.
- W4309297112 cites W2009308529 @default.
- W4309297112 cites W2022770379 @default.
- W4309297112 cites W2039725641 @default.
- W4309297112 cites W2044676948 @default.
- W4309297112 cites W2065847836 @default.
- W4309297112 cites W2078964320 @default.
- W4309297112 cites W2086294280 @default.
- W4309297112 cites W2088749722 @default.
- W4309297112 cites W2091724447 @default.
- W4309297112 cites W2101986300 @default.
- W4309297112 cites W2102278945 @default.
- W4309297112 cites W2102722567 @default.
- W4309297112 cites W2135060727 @default.
- W4309297112 cites W2137850358 @default.
- W4309297112 cites W2146803540 @default.
- W4309297112 cites W2150926065 @default.
- W4309297112 cites W2152828399 @default.
- W4309297112 cites W2161106528 @default.
- W4309297112 cites W2290168744 @default.
- W4309297112 cites W2297293651 @default.
- W4309297112 cites W2507685096 @default.
- W4309297112 cites W2542943305 @default.
- W4309297112 cites W2558981096 @default.
- W4309297112 cites W2586247640 @default.
- W4309297112 cites W2604805637 @default.
- W4309297112 cites W2627064270 @default.
- W4309297112 cites W2735962734 @default.
- W4309297112 cites W2753775066 @default.
- W4309297112 cites W2754499625 @default.
- W4309297112 cites W2765859773 @default.
- W4309297112 cites W2766254093 @default.
- W4309297112 cites W2769630486 @default.
- W4309297112 cites W2770747479 @default.
- W4309297112 cites W2782731154 @default.
- W4309297112 cites W2800770690 @default.
- W4309297112 cites W2805826733 @default.
- W4309297112 cites W2837459302 @default.
- W4309297112 cites W2889358492 @default.
- W4309297112 cites W2893050222 @default.
- W4309297112 cites W2899339826 @default.
- W4309297112 cites W2915489297 @default.
- W4309297112 cites W2923286987 @default.
- W4309297112 cites W2941333182 @default.
- W4309297112 cites W2944923640 @default.
- W4309297112 cites W2946217053 @default.
- W4309297112 cites W2974616751 @default.
- W4309297112 cites W3012451848 @default.
- W4309297112 cites W3047879827 @default.
- W4309297112 cites W3088401235 @default.
- W4309297112 cites W3127970705 @default.
- W4309297112 cites W3173764610 @default.
- W4309297112 cites W4211122720 @default.
- W4309297112 cites W4236357753 @default.
- W4309297112 doi "https://doi.org/10.7554/elife.77014" @default.
- W4309297112 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36399125" @default.
- W4309297112 hasPublicationYear "2022" @default.
- W4309297112 type Work @default.
- W4309297112 citedByCount "5" @default.
- W4309297112 countsByYear W43092971122023 @default.
- W4309297112 crossrefType "journal-article" @default.
- W4309297112 hasAuthorship W4309297112A5013054536 @default.
- W4309297112 hasAuthorship W4309297112A5013547596 @default.
- W4309297112 hasAuthorship W4309297112A5025372159 @default.
- W4309297112 hasAuthorship W4309297112A5026303073 @default.
- W4309297112 hasAuthorship W4309297112A5039339633 @default.
- W4309297112 hasAuthorship W4309297112A5042459440 @default.
- W4309297112 hasAuthorship W4309297112A5061273891 @default.