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- W4309308927 abstract "Single-domain antibody fragments, also known as VHHs or nanobodies, have opened promising avenues in therapeutics and in exploration of intracellular processes. Because of their unique structural properties, they can reach cryptic regions in their cognate antigen. Intracellular VHHs/antibodies primarily directed against cytosolic proteins or transcription factors have been described. In contrast, few of them target membrane proteins and even less recognize G protein-coupled receptors. These receptors are major therapeutic targets, which reflects their involvement in a plethora of physiological responses. Hence, they elicit a tremendous interest in the scientific community and in the industry. Comprehension of their pharmacology has been obscured by their conformational complexity, that has precluded deciphering their structural properties until the early 2010’s. To that respect, intracellular VHHs have been instrumental in stabilizing G protein-coupled receptors in active conformations in order to solve their structure, possibly bound to their primary transducers, G proteins or β-arrestins. In contrast, the modulatory properties of VHHs recognizing the intracellular regions of G protein-coupled receptors on the induced signaling network have been poorly studied. In this review, we will present the advances that the intracellular VHHs have permitted in the field of GPCR signaling and trafficking. We will also discuss the methodological hurdles that linger the discovery of modulatory intracellular VHHs directed against GPCRs, as well as the opportunities they open in drug discovery." @default.
- W4309308927 created "2022-11-26" @default.
- W4309308927 creator A5011568743 @default.
- W4309308927 creator A5050412833 @default.
- W4309308927 creator A5052480610 @default.
- W4309308927 creator A5061822159 @default.
- W4309308927 creator A5066006930 @default.
- W4309308927 creator A5079512406 @default.
- W4309308927 creator A5085194389 @default.
- W4309308927 date "2022-11-16" @default.
- W4309308927 modified "2023-10-17" @default.
- W4309308927 title "Intracellular VHHs to monitor and modulate GPCR signaling" @default.
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