FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4309361375> ?p ?o ?g. }

• W4309361375 endingPage "930" @default.
• W4309361375 startingPage "915" @default.
• W4309361375 abstract "Targeted gene therapy has become a promising approach for lung cancer treatment. In our previous work, we reported that the targeted expression of microRNA-7 (miR-7) operated by thyroid transcription factor-1 (TTF-1) promoter inhibited the growth of human lung cancer cells in vitro and in vivo; however, the intervention efficiency needed to be further improved. In this study, we identified the core promoter of TTF-1 (from −1299 bp to −871 bp) by 5′ deletion assay and screened out the putative transcription factors nuclear factor-1 (NF-1) and activator protein-1 (AP-1). Further analysis revealed that the expression level of NF-1, but not AP-1, was positively connected with the activation of TTF-1 core promoter in human non-small-cell lung cancer (NSCLC) cells. Moreover, the silencing of NF-1 could reduce the expression level of miR-7 operated by TTF-1 core promoter. Of note, we optimized four distinct sequences to form additional NF-1-binding sites (TGGCA) in the sequence of TTF-1 core promoter (termed as optTTF-1 promoter), and verified the binding efficiency of NF-1 on the optTTF-1 promoter by electrophoretic mobility shift assay (EMSA). As expected, the optTTF-1 promoter could more effectively drive miR-7 expression and inhibit the growth of human NSCLC cells in vitro, accompanied by a reduced transduction of NADH dehydrogenase (ubiquinone) 1α subcomplex 4 (NDUFA4)/protein kinase B (Akt) pathway. Consistently, optTTF-1 promoter-driven miR-7 expression could also effectively abrogate the growth and metastasis of tumor cells in a murine xenograft model of human NSCLC. Finally, no significant changes were detected in the biological indicators or the histology of some important tissues and organs, including heart, liver, and spleen. On the whole, our study revealed that the optimized TTF-1 promoter could more effectively operate miR-7 to influence the growth of human NSCLC cells, providing a new basis for the development of microRNA-based targeting gene therapy against clinical lung cancer." @default.
• W4309361375 created "2022-11-26" @default.
• W4309361375 creator A5002456827 @default.
• W4309361375 creator A5010738468 @default.
• W4309361375 creator A5015761771 @default.
• W4309361375 creator A5027195409 @default.
• W4309361375 creator A5045409917 @default.
• W4309361375 creator A5050905557 @default.
• W4309361375 creator A5054329420 @default.
• W4309361375 creator A5054518583 @default.
• W4309361375 creator A5056248574 @default.
• W4309361375 creator A5061100053 @default.
• W4309361375 creator A5064047730 @default.
• W4309361375 date "2022-11-01" @default.
• W4309361375 modified "2023-10-12" @default.
• W4309361375 title "Optimized thyroid transcription factor-1 core promoter-driven microRNA-7 expression effectively inhibits the growth of human non-small-cell lung cancer cells" @default.
• W4309361375 cites W1744771032 @default.
• W4309361375 cites W1902159756 @default.
• W4309361375 cites W1913405807 @default.
• W4309361375 cites W1966397536 @default.
• W4309361375 cites W1966510795 @default.
• W4309361375 cites W1971456303 @default.
• W4309361375 cites W1975719291 @default.
• W4309361375 cites W1979002716 @default.
• W4309361375 cites W1981827594 @default.
• W4309361375 cites W1982899514 @default.
• W4309361375 cites W1992638032 @default.
• W4309361375 cites W2032128809 @default.
• W4309361375 cites W2046207020 @default.
• W4309361375 cites W2050775788 @default.
• W4309361375 cites W2075948242 @default.
• W4309361375 cites W2083836886 @default.
• W4309361375 cites W2090227821 @default.
• W4309361375 cites W2115999568 @default.
• W4309361375 cites W2117114292 @default.
• W4309361375 cites W2130994431 @default.
• W4309361375 cites W2143584984 @default.
• W4309361375 cites W2157862813 @default.
• W4309361375 cites W2168220299 @default.
• W4309361375 cites W2194588080 @default.
• W4309361375 cites W2281710277 @default.
• W4309361375 cites W2530837075 @default.
• W4309361375 cites W2532183426 @default.
• W4309361375 cites W2561449450 @default.
• W4309361375 cites W2595814027 @default.
• W4309361375 cites W2673965378 @default.
• W4309361375 cites W2730227237 @default.
• W4309361375 cites W2743383644 @default.
• W4309361375 cites W2755801580 @default.
• W4309361375 cites W2766076173 @default.
• W4309361375 cites W2898288153 @default.
• W4309361375 cites W2903743016 @default.
• W4309361375 cites W2942446777 @default.
• W4309361375 cites W2954325121 @default.
• W4309361375 cites W3000397860 @default.
• W4309361375 cites W3014315956 @default.
• W4309361375 cites W3080277513 @default.
• W4309361375 cites W3088156317 @default.
• W4309361375 cites W3088464234 @default.
• W4309361375 cites W3092913715 @default.
• W4309361375 cites W3094926498 @default.
• W4309361375 cites W3107239072 @default.
• W4309361375 cites W3126791971 @default.
• W4309361375 cites W3135209648 @default.
• W4309361375 cites W3143515426 @default.
• W4309361375 cites W3151796084 @default.
• W4309361375 cites W3165424433 @default.
• W4309361375 cites W3204844512 @default.
• W4309361375 cites W3205519061 @default.
• W4309361375 cites W4200335180 @default.
• W4309361375 cites W4223440587 @default.
• W4309361375 cites W4248178819 @default.
• W4309361375 cites W4296747771 @default.
• W4309361375 cites W4321491322 @default.
• W4309361375 doi "https://doi.org/10.1631/jzus.b2200116" @default.
• W4309361375 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36379611" @default.
• W4309361375 hasPublicationYear "2022" @default.
• W4309361375 type Work @default.
• W4309361375 citedByCount "3" @default.
• W4309361375 countsByYear W43093613752023 @default.
• W4309361375 crossrefType "journal-article" @default.
• W4309361375 hasAuthorship W4309361375A5002456827 @default.
• W4309361375 hasAuthorship W4309361375A5010738468 @default.
• W4309361375 hasAuthorship W4309361375A5015761771 @default.
• W4309361375 hasAuthorship W4309361375A5027195409 @default.
• W4309361375 hasAuthorship W4309361375A5045409917 @default.
• W4309361375 hasAuthorship W4309361375A5050905557 @default.
• W4309361375 hasAuthorship W4309361375A5054329420 @default.
• W4309361375 hasAuthorship W4309361375A5054518583 @default.
• W4309361375 hasAuthorship W4309361375A5056248574 @default.
• W4309361375 hasAuthorship W4309361375A5061100053 @default.
• W4309361375 hasAuthorship W4309361375A5064047730 @default.
• W4309361375 hasBestOaLocation W43093613752 @default.
• W4309361375 hasConcept C101762097 @default.
• W4309361375 hasConcept C104317684 @default.
• W4309361375 hasConcept C119056186 @default.
• W4309361375 hasConcept C145059251 @default.
• W4309361375 hasConcept C150194340 @default.

• next