Matches in SemOpenAlex for { <https://semopenalex.org/work/W4309364025> ?p ?o ?g. }
- W4309364025 abstract "Engineering enzymes with novel reactivity and applying them in metabolic pathways to produce valuable products are quite challenging due to the intrinsic complexity of metabolic networks and the need for high in vivo catalytic efficiency. Triacetic acid lactone (TAL), naturally generated by 2-pyrone synthase (2PS), is a platform molecule that can be produced via microbial fermentation and further converted into value-added products. However, these conversions require extra synthetic steps under harsh conditions. We herein report a biocatalytic system for direct generation of TAL derivatives under mild conditions with controlled chemoselectivity by rationally engineering the 2PS active site and then rewiring the biocatalytic pathway in the metabolic network of E. coli to produce high-value products, such as kavalactone precursors, with yields up to 17 mg/L culture. Computer modeling indicates sterics and hydrogen-bond interactions play key roles in tuning the selectivity, efficiency and yield." @default.
- W4309364025 created "2022-11-26" @default.
- W4309364025 creator A5011303476 @default.
- W4309364025 creator A5024532179 @default.
- W4309364025 creator A5030686904 @default.
- W4309364025 creator A5035842562 @default.
- W4309364025 creator A5045648422 @default.
- W4309364025 creator A5053674630 @default.
- W4309364025 creator A5070176847 @default.
- W4309364025 date "2022-12-20" @default.
- W4309364025 modified "2023-09-26" @default.
- W4309364025 title "Reshaping the 2‐Pyrone Synthase Active Site for Chemoselective Biosynthesis of Polyketides" @default.
- W4309364025 cites W1942591007 @default.
- W4309364025 cites W1971642956 @default.
- W4309364025 cites W1994692713 @default.
- W4309364025 cites W1996629685 @default.
- W4309364025 cites W1996938184 @default.
- W4309364025 cites W2002928556 @default.
- W4309364025 cites W2034776959 @default.
- W4309364025 cites W2035150051 @default.
- W4309364025 cites W2036142593 @default.
- W4309364025 cites W2049661339 @default.
- W4309364025 cites W2068381956 @default.
- W4309364025 cites W2091007201 @default.
- W4309364025 cites W2095394114 @default.
- W4309364025 cites W2115495905 @default.
- W4309364025 cites W2117380347 @default.
- W4309364025 cites W2141158541 @default.
- W4309364025 cites W2142190505 @default.
- W4309364025 cites W2143569885 @default.
- W4309364025 cites W2149258924 @default.
- W4309364025 cites W2159489517 @default.
- W4309364025 cites W2315515937 @default.
- W4309364025 cites W2331937820 @default.
- W4309364025 cites W2331969794 @default.
- W4309364025 cites W2461926103 @default.
- W4309364025 cites W2470349522 @default.
- W4309364025 cites W2507192932 @default.
- W4309364025 cites W2516487312 @default.
- W4309364025 cites W2554528613 @default.
- W4309364025 cites W2561201681 @default.
- W4309364025 cites W2568729511 @default.
- W4309364025 cites W2580403151 @default.
- W4309364025 cites W2592504913 @default.
- W4309364025 cites W2599199696 @default.
- W4309364025 cites W2602567340 @default.
- W4309364025 cites W2614676596 @default.
- W4309364025 cites W2735208100 @default.
- W4309364025 cites W2783073377 @default.
- W4309364025 cites W2803632316 @default.
- W4309364025 cites W2898119945 @default.
- W4309364025 cites W2911459582 @default.
- W4309364025 cites W2917202476 @default.
- W4309364025 cites W2923384196 @default.
- W4309364025 cites W2949602129 @default.
- W4309364025 cites W2962765091 @default.
- W4309364025 cites W2971267407 @default.
- W4309364025 cites W3036820025 @default.
- W4309364025 cites W3118519301 @default.
- W4309364025 cites W3149167195 @default.
- W4309364025 cites W3193199994 @default.
- W4309364025 cites W4206824777 @default.
- W4309364025 cites W4283216625 @default.
- W4309364025 cites W4285007601 @default.
- W4309364025 cites W4285008117 @default.
- W4309364025 cites W4287509999 @default.
- W4309364025 cites W4309364025 @default.
- W4309364025 doi "https://doi.org/10.1002/anie.202212440" @default.
- W4309364025 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36398563" @default.
- W4309364025 hasPublicationYear "2022" @default.
- W4309364025 type Work @default.
- W4309364025 citedByCount "2" @default.
- W4309364025 countsByYear W43093640252022 @default.
- W4309364025 countsByYear W43093640252023 @default.
- W4309364025 crossrefType "journal-article" @default.
- W4309364025 hasAuthorship W4309364025A5011303476 @default.
- W4309364025 hasAuthorship W4309364025A5024532179 @default.
- W4309364025 hasAuthorship W4309364025A5030686904 @default.
- W4309364025 hasAuthorship W4309364025A5035842562 @default.
- W4309364025 hasAuthorship W4309364025A5045648422 @default.
- W4309364025 hasAuthorship W4309364025A5053674630 @default.
- W4309364025 hasAuthorship W4309364025A5070176847 @default.
- W4309364025 hasBestOaLocation W43093640251 @default.
- W4309364025 hasConcept C100544194 @default.
- W4309364025 hasConcept C147816474 @default.
- W4309364025 hasConcept C161790260 @default.
- W4309364025 hasConcept C181199279 @default.
- W4309364025 hasConcept C185592680 @default.
- W4309364025 hasConcept C190667039 @default.
- W4309364025 hasConcept C191503008 @default.
- W4309364025 hasConcept C191908910 @default.
- W4309364025 hasConcept C192989942 @default.
- W4309364025 hasConcept C21951064 @default.
- W4309364025 hasConcept C2776612806 @default.
- W4309364025 hasConcept C2778457124 @default.
- W4309364025 hasConcept C41183919 @default.
- W4309364025 hasConcept C55493867 @default.
- W4309364025 hasConcept C6350086 @default.
- W4309364025 hasConcept C65024703 @default.
- W4309364025 hasConcept C70721500 @default.