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• W4309366984 abstract "There are various approaches in which one can isolate microglia from murine brains, such as immunomagnetic, density gradient, FACS and differential adhesive methods. In this procedure a modified flask-tapping approach was used due to its simplicity and reproducibility. Our protocol requires only a single step to isolate the microglia from the mixed cell population. Once the microglia were isolated, we characterized cell purity, microglial morphology and phagocytic activity. The single-step protocol, without the need for additional astrocyte or oligodendrocyte separation, allows microglial cells to be used immediately for experimental purposes. The protocol is low-cost and can be performed in any lab with standard cell-culture equipment." @default.
• W4309366984 created "2022-11-26" @default.
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• W4309366984 date "2022-12-01" @default.
• W4309366984 modified "2023-09-25" @default.
• W4309366984 title "A simplified procedure for isolation of primary murine microglia" @default.
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• W4309366984 doi "https://doi.org/10.2144/btn-2022-0054" @default.
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