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- W4309367761 endingPage "2202376" @default.
- W4309367761 startingPage "2202376" @default.
- W4309367761 abstract "Gingival crevice and gingival crevicular fluid (GCF) flow play a crucial role at the gingiva-oral microbiome interface which contributes toward maintaining the balance between gingival health and periodontal disease. Interstitial flow of GCF strongly impacts the host-microbiome interactions and tissue responses. However, currently available in vitro preclinical models largely disregard the dynamic nature of gingival crevicular microenvironment, thus limiting the progress in the development of periodontal therapeutics. Here, a proof-of-principle gingival crevice-on-chip microfluidic platform to culture gingival connective tissue equivalent (CTE) under dynamic interstitial fluid flow mimicking the GCF is described. On-chip co-culture using oral symbiont (Streptococcus oralis) shows the potential to recapitulate microbial colonization, formation of biofilm-like structures at the tissue-microbiome interface, long-term co-culture, and bacterial clearance secondary to simulated GCF (s-GCF) flow. Further, on-chip exposure of the gingival CTEs to the toll-like receptor-2 (TLR-2) agonist or periodontal pathogen Fusobacterium nucleatum demonstrates the potential to mimic early gingival inflammation. In contrast to direct exposure, the induction of s-GCF flow toward the bacterial front attenuates the secretion of inflammatory mediators demonstrating the protective effect of GCF flow. This proposed in vitro platform offers the potential to study complex host-microbe interactions in periodontal disease and the development of periodontal therapeutics under near-microphysiological conditions." @default.
- W4309367761 created "2022-11-26" @default.
- W4309367761 creator A5009422081 @default.
- W4309367761 creator A5043397723 @default.
- W4309367761 creator A5057915644 @default.
- W4309367761 creator A5068940854 @default.
- W4309367761 creator A5080135917 @default.
- W4309367761 date "2022-11-28" @default.
- W4309367761 modified "2023-09-30" @default.
- W4309367761 title "Modeling Crevicular Fluid Flow and Host‐Oral Microbiome Interactions in a Gingival Crevice‐on‐Chip" @default.
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