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- W4309387332 abstract "Abstract Background Obligate blood-feeding insects obtain the nutrients and water necessary to ensure survival from the vertebrate blood. The internal taste sensilla, situated in the pharynx, evaluate the suitability of the ingested food. Here, through multiple approaches, we characterized the pharyngeal organ (PO) of the hematophagous kissing bug Rhodnius prolixus to determine its role in food assessment. The PO, located antero-dorsally in the pharynx, comprises 8 taste sensilla that become bathed with the incoming blood. Results We showed that these taste sensilla house gustatory receptor neurons projecting their axons through the labral nerves to reach the subesophageal zone in the brain. We found that these neurons are electrically activated by relevant appetitive and aversive gustatory stimuli such as NaCl, ATP and caffeine. Using RNA-Seq, we examined the expression of sensory-related gene families in the PO. We identified gustatory receptors, ionotropic receptors, transient receptor potential channels, pickpocket channels, opsins, takeouts, neuropeptide precursors, neuropeptide receptors and biogenic amine receptors. RNA interference assays demonstrated that the pickpocket channel Rproppk014276 is necessary for salt detection during feeding. Conclusion We provide evidence of the role of the pharyngeal organ in food evaluation. This work shows the first comprehensive characterization of a pharyngeal taste organ in a hematophagous insect." @default.
- W4309387332 created "2022-11-26" @default.
- W4309387332 creator A5001644662 @default.
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- W4309387332 creator A5057091901 @default.
- W4309387332 date "2022-11-15" @default.
- W4309387332 modified "2023-10-08" @default.
- W4309387332 title "Food recognition in a blood-feeding insect: characterization of the pharyngeal taste organ" @default.
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