FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4309402452> ?p ?o ?g. }

• W4309402452 endingPage "100077" @default.
• W4309402452 startingPage "100077" @default.
• W4309402452 abstract "Many amyloid-forming proteins, which are normally intrinsically disordered, undergo a disorder-to-order transition to form fibrils with a rigid β-sheet core flanked by disordered domains. Solid-state NMR (ssNMR) and cryogenic electron microscopy (cryoEM) excel at resolving the rigid structures within amyloid cores but studying the dynamically disordered domains remains challenging. This challenge is exemplified by mutant huntingtin exon 1 (HttEx1), which self-assembles into pathogenic neuronal inclusions in Huntington disease (HD). The mutant protein’s expanded polyglutamine (polyQ) segment forms a fibril core that is rigid and sequestered from the solvent. Beyond the core, solvent-exposed surface residues mediate biological interactions and other properties of fibril polymorphs. Here we deploy magic angle spinning ssNMR experiments to probe for semi-rigid residues proximal to the fibril core and examine how solvent dynamics impact the fibrils’ segmental dynamics. Dynamic spectral editing (DYSE) 2D ssNMR based on a combination of cross-polarization (CP) ssNMR with selective dipolar dephasing reveals the weak signals of solvent-mobilized glutamine residues, while suppressing the normally strong background of rigid core signals. This type of ‘intermediate motion selection’ (IMS) experiment based on cross-polarization (CP) ssNMR, is complementary to INEPT- and CP-based measurements that highlight highly flexible or highly rigid protein segments, respectively. Integration of the IMS-DYSE element in standard CP-based ssNMR experiments permits the observation of semi-rigid residues in a variety of contexts, including in membrane proteins and protein complexes. We discuss the relevance of semi-rigid solvent-facing residues outside the fibril core to the latter’s detection with specific dyes and positron emission tomography tracers." @default.
• W4309402452 created "2022-11-26" @default.
• W4309402452 creator A5039271010 @default.
• W4309402452 creator A5090166266 @default.
• W4309402452 creator A5091686211 @default.
• W4309402452 date "2022-01-01" @default.
• W4309402452 modified "2023-09-25" @default.
• W4309402452 title "Selective observation of semi-rigid non-core residues in dynamically complex mutant huntingtin protein fibrils" @default.
• W4309402452 cites W1901715948 @default.
• W4309402452 cites W1945465158 @default.
• W4309402452 cites W1966457778 @default.
• W4309402452 cites W1966910457 @default.
• W4309402452 cites W1976077400 @default.
• W4309402452 cites W1979367436 @default.
• W4309402452 cites W1996667889 @default.
• W4309402452 cites W1998122246 @default.
• W4309402452 cites W2002735780 @default.
• W4309402452 cites W2002869713 @default.
• W4309402452 cites W2012783823 @default.
• W4309402452 cites W2014668312 @default.
• W4309402452 cites W2015795135 @default.
• W4309402452 cites W2023338599 @default.
• W4309402452 cites W2031034110 @default.
• W4309402452 cites W2032522569 @default.
• W4309402452 cites W2035751953 @default.
• W4309402452 cites W2042197472 @default.
• W4309402452 cites W2053460134 @default.
• W4309402452 cites W2061612744 @default.
• W4309402452 cites W2079250951 @default.
• W4309402452 cites W2086565576 @default.
• W4309402452 cites W2121642311 @default.
• W4309402452 cites W2131926693 @default.
• W4309402452 cites W2137450588 @default.
• W4309402452 cites W2138233796 @default.
• W4309402452 cites W2144535585 @default.
• W4309402452 cites W2147282851 @default.
• W4309402452 cites W2155779385 @default.
• W4309402452 cites W2163210644 @default.
• W4309402452 cites W2253321251 @default.
• W4309402452 cites W2272324466 @default.
• W4309402452 cites W2317462515 @default.
• W4309402452 cites W2318414036 @default.
• W4309402452 cites W2426994386 @default.
• W4309402452 cites W2521830278 @default.
• W4309402452 cites W2547307701 @default.
• W4309402452 cites W2564716435 @default.
• W4309402452 cites W2569594520 @default.
• W4309402452 cites W2595081881 @default.
• W4309402452 cites W2614053601 @default.
• W4309402452 cites W2617139971 @default.
• W4309402452 cites W2625296422 @default.
• W4309402452 cites W2727929522 @default.
• W4309402452 cites W2751898404 @default.
• W4309402452 cites W2752648229 @default.
• W4309402452 cites W2758396775 @default.
• W4309402452 cites W2763125444 @default.
• W4309402452 cites W2788389336 @default.
• W4309402452 cites W2791074822 @default.
• W4309402452 cites W2795307962 @default.
• W4309402452 cites W2802839169 @default.
• W4309402452 cites W2892700278 @default.
• W4309402452 cites W2920459656 @default.
• W4309402452 cites W2945927462 @default.
• W4309402452 cites W2950980718 @default.
• W4309402452 cites W2952452438 @default.
• W4309402452 cites W2976335279 @default.
• W4309402452 cites W2981898616 @default.
• W4309402452 cites W3000505702 @default.
• W4309402452 cites W3011713314 @default.
• W4309402452 cites W3016287419 @default.
• W4309402452 cites W3038113316 @default.
• W4309402452 cites W3043057094 @default.
• W4309402452 cites W3093640802 @default.
• W4309402452 cites W3103611992 @default.
• W4309402452 cites W3110085791 @default.
• W4309402452 cites W3129048863 @default.
• W4309402452 cites W3132865190 @default.
• W4309402452 cites W3179951414 @default.
• W4309402452 cites W3181826210 @default.
• W4309402452 cites W3182916186 @default.
• W4309402452 cites W4205120276 @default.
• W4309402452 cites W4206731227 @default.
• W4309402452 cites W4210514235 @default.
• W4309402452 cites W4211110682 @default.
• W4309402452 cites W4282912979 @default.
• W4309402452 doi "https://doi.org/10.1016/j.yjsbx.2022.100077" @default.
• W4309402452 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36419510" @default.
• W4309402452 hasPublicationYear "2022" @default.
• W4309402452 type Work @default.
• W4309402452 citedByCount "1" @default.
• W4309402452 countsByYear W43094024522023 @default.
• W4309402452 crossrefType "journal-article" @default.
• W4309402452 hasAuthorship W4309402452A5039271010 @default.
• W4309402452 hasAuthorship W4309402452A5090166266 @default.
• W4309402452 hasAuthorship W4309402452A5091686211 @default.
• W4309402452 hasBestOaLocation W43094024521 @default.
• W4309402452 hasConcept C104317684 @default.
• W4309402452 hasConcept C121332964 @default.

• next