FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4309491690> ?p ?o ?g. }

• W4309491690 endingPage "277" @default.
• W4309491690 startingPage "263" @default.
• W4309491690 abstract "Phosphorylated tau is the main protein present in neurofibrillary tangles, the presence of which is a key neuropathological hallmark of Alzheimer’s disease (AD). The toxic effects of phosphorylated tau are likely mediated by interacting proteins; however, methods to identify these interacting proteins comprehensively in human brain tissue are limited. Here, we describe a method that enables the efficient identification of hundreds of proteins that interact with phosphorylated tau (pTau), using affinity purification–mass spectrometry (AP-MS) on human, fresh-frozen brain tissue from donors with AD. Tissue is homogenized using a gentle technique that preserves protein–protein interactions, and co-immunoprecipitation of pTau and its interacting proteins is performed using the PHF1 antibody. The resulting protein interactors are then identified using label-free quantitative liquid chromatography-mass spectrometry (LC-MS)/MS. The Significance Analysis of INTeractome (SAINT) algorithm is used to determine which proteins significantly interact with pTau. This approach enables the detection of an abundance of all 6 isoforms of tau, 23 phosphorylated residues on tau, and 125 significant pTau protein interactors, in human AD brain tissue." @default.
• W4309491690 created "2022-11-28" @default.
• W4309491690 creator A5014952212 @default.
• W4309491690 creator A5046586712 @default.
• W4309491690 creator A5060656369 @default.
• W4309491690 creator A5063931891 @default.
• W4309491690 date "2022-11-19" @default.
• W4309491690 modified "2023-10-17" @default.
• W4309491690 title "Use of Affinity Purification–Mass Spectrometry to Identify Phosphorylated Tau Interactors in Alzheimer’s Disease" @default.
• W4309491690 cites W1567464267 @default.
• W4309491690 cites W1930179398 @default.
• W4309491690 cites W1984453732 @default.
• W4309491690 cites W2027619670 @default.
• W4309491690 cites W2047077861 @default.
• W4309491690 cites W2048560678 @default.
• W4309491690 cites W2074274706 @default.
• W4309491690 cites W2091829752 @default.
• W4309491690 cites W2128228199 @default.
• W4309491690 cites W2207904128 @default.
• W4309491690 cites W2482317947 @default.
• W4309491690 cites W2579528010 @default.
• W4309491690 cites W2887193453 @default.
• W4309491690 cites W2912165492 @default.
• W4309491690 cites W2944309866 @default.
• W4309491690 cites W2984978709 @default.
• W4309491690 cites W2992077194 @default.
• W4309491690 cites W3078141069 @default.
• W4309491690 cites W3147228812 @default.
• W4309491690 cites W4206774749 @default.
• W4309491690 doi "https://doi.org/10.1007/978-1-0716-2655-9_14" @default.
• W4309491690 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36399275" @default.
• W4309491690 hasPublicationYear "2022" @default.
• W4309491690 type Work @default.
• W4309491690 citedByCount "4" @default.
• W4309491690 countsByYear W43094916902022 @default.
• W4309491690 countsByYear W43094916902023 @default.
• W4309491690 crossrefType "book-chapter" @default.
• W4309491690 hasAuthorship W4309491690A5014952212 @default.
• W4309491690 hasAuthorship W4309491690A5046586712 @default.
• W4309491690 hasAuthorship W4309491690A5060656369 @default.
• W4309491690 hasAuthorship W4309491690A5063931891 @default.
• W4309491690 hasConcept C104317684 @default.
• W4309491690 hasConcept C11804247 @default.
• W4309491690 hasConcept C11960822 @default.
• W4309491690 hasConcept C142724271 @default.
• W4309491690 hasConcept C162356407 @default.
• W4309491690 hasConcept C169760540 @default.
• W4309491690 hasConcept C185592680 @default.
• W4309491690 hasConcept C2777670902 @default.
• W4309491690 hasConcept C2778670691 @default.
• W4309491690 hasConcept C2779134260 @default.
• W4309491690 hasConcept C43617362 @default.
• W4309491690 hasConcept C46111723 @default.
• W4309491690 hasConcept C502032728 @default.
• W4309491690 hasConcept C53345823 @default.
• W4309491690 hasConcept C55493867 @default.
• W4309491690 hasConcept C71829478 @default.
• W4309491690 hasConcept C71924100 @default.
• W4309491690 hasConcept C86803240 @default.
• W4309491690 hasConcept C95444343 @default.
• W4309491690 hasConcept C96970771 @default.
• W4309491690 hasConcept C9760119 @default.
• W4309491690 hasConceptScore W4309491690C104317684 @default.
• W4309491690 hasConceptScore W4309491690C11804247 @default.
• W4309491690 hasConceptScore W4309491690C11960822 @default.
• W4309491690 hasConceptScore W4309491690C142724271 @default.
• W4309491690 hasConceptScore W4309491690C162356407 @default.
• W4309491690 hasConceptScore W4309491690C169760540 @default.
• W4309491690 hasConceptScore W4309491690C185592680 @default.
• W4309491690 hasConceptScore W4309491690C2777670902 @default.
• W4309491690 hasConceptScore W4309491690C2778670691 @default.
• W4309491690 hasConceptScore W4309491690C2779134260 @default.
• W4309491690 hasConceptScore W4309491690C43617362 @default.
• W4309491690 hasConceptScore W4309491690C46111723 @default.
• W4309491690 hasConceptScore W4309491690C502032728 @default.
• W4309491690 hasConceptScore W4309491690C53345823 @default.
• W4309491690 hasConceptScore W4309491690C55493867 @default.
• W4309491690 hasConceptScore W4309491690C71829478 @default.
• W4309491690 hasConceptScore W4309491690C71924100 @default.
• W4309491690 hasConceptScore W4309491690C86803240 @default.
• W4309491690 hasConceptScore W4309491690C95444343 @default.
• W4309491690 hasConceptScore W4309491690C96970771 @default.
• W4309491690 hasConceptScore W4309491690C9760119 @default.
• W4309491690 hasLocation W43094916901 @default.
• W4309491690 hasLocation W43094916902 @default.
• W4309491690 hasOpenAccess W4309491690 @default.
• W4309491690 hasPrimaryLocation W43094916901 @default.
• W4309491690 hasRelatedWork W2110065729 @default.
• W4309491690 hasRelatedWork W2116068118 @default.
• W4309491690 hasRelatedWork W2273923068 @default.
• W4309491690 hasRelatedWork W2355009881 @default.
• W4309491690 hasRelatedWork W2921179630 @default.
• W4309491690 hasRelatedWork W2989548361 @default.
• W4309491690 hasRelatedWork W3133083146 @default.
• W4309491690 hasRelatedWork W4296250434 @default.
• W4309491690 hasRelatedWork W4309491690 @default.
• W4309491690 hasRelatedWork W4323825539 @default.
• W4309491690 isParatext "false" @default.

• next