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- W4309582786 abstract "Abstract The concentration of human immunoglobulin E (IgE) in blood is an indicator for diagnosing an allergy. For detecting human IgE by an electrochemical immunosensor, redox mediator molecules were modified on a gold electrode using thiolated organic molecules, which can form self‐assembled monolayers on the electrode surface. Ferrocene was used as a redox mediator, and the current signal was amplified via redox cycling using 4‐aminophenol. Measurements were obtained after the immobilization of the target‐captured antibody, target human IgE, biotin‐conjugated secondary antibody, and avidin alkaline phosphatase in a sandwich‐type immunosensor configuration. The prepared immunosensor exhibited a limit of detection of 1 IU/mL and dynamic range of 3–30 IU/mL." @default.
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- W4309582786 date "2022-11-18" @default.
- W4309582786 modified "2023-10-14" @default.
- W4309582786 title "Immunosensor for human <scp>IgE</scp> detection using electrochemical redox cycling with ferrocene‐mixed self‐assembled monolayers modified Au electrode" @default.
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- W4309582786 doi "https://doi.org/10.1002/bkcs.12641" @default.
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