Matches in SemOpenAlex for { <https://semopenalex.org/work/W4309593294> ?p ?o ?g. }
- W4309593294 endingPage "111682" @default.
- W4309593294 startingPage "111682" @default.
- W4309593294 abstract "In vitro studies suggest that mapping the spatiotemporal complexity of nuclear factor κB (NF-κB) signaling is essential to understanding its function. The lack of tools to directly monitor NF-κB proteins in vivo has hindered such efforts. Here, we introduce reporter mice with the endogenous RelA (p65) or c-Rel labeled with distinct fluorescent proteins and a double knockin with both subunits labeled. Overcoming hurdles in simultaneous live-cell imaging of RelA and c-Rel, we show that quantitative features of signaling reflect the identity of activating ligands, differ between primary and immortalized cells, and shift toward c-Rel in microglia from aged brains. RelA:c-Rel heterodimer is unexpectedly depleted in the nuclei of stimulated cells. Trajectories of subunit co-expression in immune lineages reveal a reduction at key cell maturation stages. These results demonstrate the power of these reporters in gaining deeper insights into NF-κB biology, with the spectral complementarity of the labeled NF-κB proteins enabling diverse applications." @default.
- W4309593294 created "2022-11-28" @default.
- W4309593294 creator A5003713629 @default.
- W4309593294 creator A5003965496 @default.
- W4309593294 creator A5017948966 @default.
- W4309593294 creator A5019425820 @default.
- W4309593294 creator A5024644145 @default.
- W4309593294 creator A5025665707 @default.
- W4309593294 creator A5028354022 @default.
- W4309593294 creator A5031598843 @default.
- W4309593294 creator A5040049345 @default.
- W4309593294 creator A5042213683 @default.
- W4309593294 creator A5054010038 @default.
- W4309593294 creator A5056916013 @default.
- W4309593294 creator A5088777789 @default.
- W4309593294 creator A5088853072 @default.
- W4309593294 date "2022-11-01" @default.
- W4309593294 modified "2023-10-14" @default.
- W4309593294 title "Double knockin mice show NF-κB trajectories in immune signaling and aging" @default.
- W4309593294 cites W1605318274 @default.
- W4309593294 cites W1621475458 @default.
- W4309593294 cites W1911775654 @default.
- W4309593294 cites W1968263458 @default.
- W4309593294 cites W1968916032 @default.
- W4309593294 cites W1975598191 @default.
- W4309593294 cites W1980154709 @default.
- W4309593294 cites W1982062664 @default.
- W4309593294 cites W1993482839 @default.
- W4309593294 cites W1998463022 @default.
- W4309593294 cites W2021387539 @default.
- W4309593294 cites W2031536129 @default.
- W4309593294 cites W2066218351 @default.
- W4309593294 cites W2074670317 @default.
- W4309593294 cites W2080562735 @default.
- W4309593294 cites W2088394793 @default.
- W4309593294 cites W2094863064 @default.
- W4309593294 cites W2095774239 @default.
- W4309593294 cites W2095879596 @default.
- W4309593294 cites W2099419435 @default.
- W4309593294 cites W2109527049 @default.
- W4309593294 cites W2115281458 @default.
- W4309593294 cites W2124836944 @default.
- W4309593294 cites W2125937596 @default.
- W4309593294 cites W2127680889 @default.
- W4309593294 cites W2138934663 @default.
- W4309593294 cites W2140053284 @default.
- W4309593294 cites W2146336227 @default.
- W4309593294 cites W2166096831 @default.
- W4309593294 cites W2170107603 @default.
- W4309593294 cites W2235108513 @default.
- W4309593294 cites W2411288489 @default.
- W4309593294 cites W2503872635 @default.
- W4309593294 cites W2551836638 @default.
- W4309593294 cites W2555095546 @default.
- W4309593294 cites W2563416245 @default.
- W4309593294 cites W2566910492 @default.
- W4309593294 cites W2604169508 @default.
- W4309593294 cites W2604482202 @default.
- W4309593294 cites W2767212349 @default.
- W4309593294 cites W2927664050 @default.
- W4309593294 cites W2932327961 @default.
- W4309593294 cites W2943631740 @default.
- W4309593294 cites W2981450715 @default.
- W4309593294 cites W3008964404 @default.
- W4309593294 cites W3037464292 @default.
- W4309593294 cites W3161504380 @default.
- W4309593294 cites W3202682460 @default.
- W4309593294 cites W3204360192 @default.
- W4309593294 cites W4206511670 @default.
- W4309593294 cites W4292121795 @default.
- W4309593294 doi "https://doi.org/10.1016/j.celrep.2022.111682" @default.
- W4309593294 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36417863" @default.
- W4309593294 hasPublicationYear "2022" @default.
- W4309593294 type Work @default.
- W4309593294 citedByCount "4" @default.
- W4309593294 countsByYear W43095932942021 @default.
- W4309593294 countsByYear W43095932942023 @default.
- W4309593294 crossrefType "journal-article" @default.
- W4309593294 hasAuthorship W4309593294A5003713629 @default.
- W4309593294 hasAuthorship W4309593294A5003965496 @default.
- W4309593294 hasAuthorship W4309593294A5017948966 @default.
- W4309593294 hasAuthorship W4309593294A5019425820 @default.
- W4309593294 hasAuthorship W4309593294A5024644145 @default.
- W4309593294 hasAuthorship W4309593294A5025665707 @default.
- W4309593294 hasAuthorship W4309593294A5028354022 @default.
- W4309593294 hasAuthorship W4309593294A5031598843 @default.
- W4309593294 hasAuthorship W4309593294A5040049345 @default.
- W4309593294 hasAuthorship W4309593294A5042213683 @default.
- W4309593294 hasAuthorship W4309593294A5054010038 @default.
- W4309593294 hasAuthorship W4309593294A5056916013 @default.
- W4309593294 hasAuthorship W4309593294A5088777789 @default.
- W4309593294 hasAuthorship W4309593294A5088853072 @default.
- W4309593294 hasBestOaLocation W43095932942 @default.
- W4309593294 hasConcept C104292427 @default.
- W4309593294 hasConcept C104317684 @default.
- W4309593294 hasConcept C16613235 @default.
- W4309593294 hasConcept C2777730290 @default.
- W4309593294 hasConcept C54355233 @default.