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• W4309600483 endingPage "e0261595" @default.
• W4309600483 startingPage "e0261595" @default.
• W4309600483 abstract "Bovine brucellosis is endemic in Rwanda, although, there is a paucity of documented evidence about the disease in slaughtered cattle. A cross-sectional study was conducted in slaughtered cattle (n = 300) to determine the seroprevalence of anti- Brucella antibodies using the Rose Bengal Test (RBT), and indirect enzyme-linked immunosorbent assay (i-ELISA). Corresponding tissues were cultured onto a modified Centro de Investigación y Tecnología Agroalimentaria (CITA) selective medium and analysed for Brucella spp. using the 16S-23S ribosomal interspacer region (ITS), AMOS, and Bruce-ladder PCR assays. The seroprevalence was 20.7% (62/300) with RBT, 2.9% (8/300) with i-ELISA, and 2.9% (8/300) using both tests in series. Brucella -specific 16S-23S ribosomal DNA interspace region (ITS) PCR detected Brucella DNA in 5.6% (17/300; Brucella culture prevalence). AMOS-PCR assay identified mixed B . abortus and B . melitensis (n = 3), B . abortus (n = 3) and B . melitensis (n = 5) while Bruce-ladder PCR also identified B . abortus (n = 5) and B . melitensis (n = 6). The gold standard culture method combined with PCR confirmation identified 5.6% Brucella cultures and this culture prevalence is higher than the more sensitive seroprevalence of 2.9%. This emphasizes the need to validate the serological tests in Rwanda. The mixed infection caused by B . abortus and B . melitensis in slaughtered cattle indicates cross-infection and poses a risk of exposure potential to abattoir workers. It is essential to urgently strengthen a coordinated national bovine brucellosis vaccination and initiate a test-and-slaughter program that is not presently applicable in Rwanda." @default.
• W4309600483 created "2022-11-28" @default.
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• W4309600483 date "2022-11-22" @default.
• W4309600483 modified "2023-10-14" @default.
• W4309600483 title "Seroprevalence of brucellosis and molecular characterization of Brucella spp. from slaughtered cattle in Rwanda" @default.
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• W4309600483 doi "https://doi.org/10.1371/journal.pone.0261595" @default.
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