Matches in SemOpenAlex for { <https://semopenalex.org/work/W4309609430> ?p ?o ?g. }
- W4309609430 abstract "Four modes of endocytosis and subsequent synaptic vesicle (SV) recycling have been described at the presynapse to ensure the availability of SVs for synaptic release. However, it is unclear to what extend these modes operate under physiological activity patterns in vivo. The coat protein clathrin can regenerate SVs either directly from the plasma membrane (PM) via clathrin-mediated endocytosis (CME), or indirectly from synaptic endosomes by SV budding. Here, we examined the role of clathrin in SV recycling under physiological conditions by applying the clathrin inhibitor Pitstop-2 to the calyx of Held, a synapse optimized for high frequency synaptic transmission in the auditory brainstem, in vivo. The effects of clathrin-inhibition on SV recycling were investigated by serial sectioning scanning electron microscopy (S3EM) and 3D reconstructions of endocytic structures labeled by the endocytosis marker horseradish peroxidase (HRP). We observed large endosomal compartments as well as HRP-filled, black SVs (bSVs) that have been recently recycled. The application of Pitstop-2 led to reduced bSV but not large endosome density, increased volumes of large endosomes and shifts in the localization of both types of endocytic compartments within the synapse. These changes after perturbation of clathrin function suggest that clathrin plays a role in SV recycling from both, the PM and large endosomes, under physiological activity patterns, in vivo." @default.
- W4309609430 created "2022-11-28" @default.
- W4309609430 creator A5005508666 @default.
- W4309609430 creator A5034515187 @default.
- W4309609430 creator A5038261044 @default.
- W4309609430 creator A5065879617 @default.
- W4309609430 creator A5085096681 @default.
- W4309609430 creator A5089289289 @default.
- W4309609430 date "2022-11-17" @default.
- W4309609430 modified "2023-09-26" @default.
- W4309609430 title "Effects of the clathrin inhibitor Pitstop-2 on synaptic vesicle recycling at a central synapse in vivo" @default.
- W4309609430 cites W1491063646 @default.
- W4309609430 cites W1500110626 @default.
- W4309609430 cites W1664184517 @default.
- W4309609430 cites W1886198059 @default.
- W4309609430 cites W1967302106 @default.
- W4309609430 cites W1974134481 @default.
- W4309609430 cites W1976161883 @default.
- W4309609430 cites W1977898869 @default.
- W4309609430 cites W1978923377 @default.
- W4309609430 cites W1983061726 @default.
- W4309609430 cites W2006766519 @default.
- W4309609430 cites W2008315205 @default.
- W4309609430 cites W2008643711 @default.
- W4309609430 cites W2011652925 @default.
- W4309609430 cites W2017151717 @default.
- W4309609430 cites W2022802454 @default.
- W4309609430 cites W2028130617 @default.
- W4309609430 cites W2029980283 @default.
- W4309609430 cites W2031722646 @default.
- W4309609430 cites W2048878540 @default.
- W4309609430 cites W2058153312 @default.
- W4309609430 cites W2060346226 @default.
- W4309609430 cites W2062829534 @default.
- W4309609430 cites W2079324871 @default.
- W4309609430 cites W2083279393 @default.
- W4309609430 cites W2090416745 @default.
- W4309609430 cites W2093450191 @default.
- W4309609430 cites W2096575464 @default.
- W4309609430 cites W2098703603 @default.
- W4309609430 cites W2105172681 @default.
- W4309609430 cites W2135461141 @default.
- W4309609430 cites W2135719485 @default.
- W4309609430 cites W2137802705 @default.
- W4309609430 cites W2140552779 @default.
- W4309609430 cites W2144107809 @default.
- W4309609430 cites W2153309256 @default.
- W4309609430 cites W2153492531 @default.
- W4309609430 cites W2154156417 @default.
- W4309609430 cites W2156312157 @default.
- W4309609430 cites W2169799322 @default.
- W4309609430 cites W2171264494 @default.
- W4309609430 cites W2172095199 @default.
- W4309609430 cites W2231550233 @default.
- W4309609430 cites W2305957222 @default.
- W4309609430 cites W2339057475 @default.
- W4309609430 cites W2543675020 @default.
- W4309609430 cites W2590662729 @default.
- W4309609430 cites W2604690670 @default.
- W4309609430 cites W2616969711 @default.
- W4309609430 cites W2791550087 @default.
- W4309609430 cites W2799552138 @default.
- W4309609430 cites W2803574456 @default.
- W4309609430 cites W2903642104 @default.
- W4309609430 cites W2945002616 @default.
- W4309609430 cites W2980728936 @default.
- W4309609430 cites W3126032609 @default.
- W4309609430 cites W3185242226 @default.
- W4309609430 cites W4214550233 @default.
- W4309609430 cites W4226054361 @default.
- W4309609430 doi "https://doi.org/10.3389/fnsyn.2022.1056308" @default.
- W4309609430 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36466146" @default.
- W4309609430 hasPublicationYear "2022" @default.
- W4309609430 type Work @default.
- W4309609430 citedByCount "3" @default.
- W4309609430 countsByYear W43096094302023 @default.
- W4309609430 crossrefType "journal-article" @default.
- W4309609430 hasAuthorship W4309609430A5005508666 @default.
- W4309609430 hasAuthorship W4309609430A5034515187 @default.
- W4309609430 hasAuthorship W4309609430A5038261044 @default.
- W4309609430 hasAuthorship W4309609430A5065879617 @default.
- W4309609430 hasAuthorship W4309609430A5085096681 @default.
- W4309609430 hasAuthorship W4309609430A5089289289 @default.
- W4309609430 hasBestOaLocation W43096094301 @default.
- W4309609430 hasConcept C102747710 @default.
- W4309609430 hasConcept C127445978 @default.
- W4309609430 hasConcept C130316041 @default.
- W4309609430 hasConcept C148785051 @default.
- W4309609430 hasConcept C1491633281 @default.
- W4309609430 hasConcept C169760540 @default.
- W4309609430 hasConcept C170493617 @default.
- W4309609430 hasConcept C181199279 @default.
- W4309609430 hasConcept C185592680 @default.
- W4309609430 hasConcept C191597335 @default.
- W4309609430 hasConcept C200170125 @default.
- W4309609430 hasConcept C2777676563 @default.
- W4309609430 hasConcept C28005876 @default.
- W4309609430 hasConcept C2908668639 @default.
- W4309609430 hasConcept C41625074 @default.
- W4309609430 hasConcept C55493867 @default.