Matches in SemOpenAlex for { <https://semopenalex.org/work/W4309657079> ?p ?o ?g. }
- W4309657079 endingPage "3681" @default.
- W4309657079 startingPage "3671" @default.
- W4309657079 abstract "Enhancers involved in the upregulation of multiple oncogenes play a fundamental role in tumorigenesis and immortalization. Exploring the activity of enhancers in living cells has emerged as a critical path to a deep understanding of cancer properties, further providing important clues to targeted therapy. However, identifying enhancer activity in living cells is challenging due to the double biological barriers of a cell cytoplasmic membrane and a nuclear membrane, limiting the sensitivity and responsiveness of conventional probing methods. In this work, we developed a nanoelectroporation-probing (NP) platform, which enables intranuclear probe delivery for sensitive interrogation of enhancer activity in living cells. The nanoelectroporation biochip achieved highly focused perforation of the cell cytoplasmic membrane and brought about additional driving force to expedite the delivery of probes into the nucleus. The probes targeting enhancer activity (named “PH probe”) are programmed with a cyclic amplification strategy and enable an increase in the fluorescence signals over 100-fold within 1 h. The platform was leveraged to detect the activity of CCAT1 enhancers (CCAT1, colon cancer-associated transcript-1, a long noncoding RNA that functions in tumor invasion and metastasis) in cell samples from clinical lung cancer patients, as well as reveal the heterogeneity of enhancers among different patients. The observations may extend the linkages between enhancers and cancer cells while validating the robustness and reliability of the platform for the assay of enhancer activity. This platform will be a promising toolbox with wide applicable potential for the intranuclear study of living cells." @default.
- W4309657079 created "2022-11-29" @default.
- W4309657079 creator A5010734957 @default.
- W4309657079 creator A5012485751 @default.
- W4309657079 creator A5014528965 @default.
- W4309657079 creator A5038830852 @default.
- W4309657079 creator A5042591538 @default.
- W4309657079 creator A5054619417 @default.
- W4309657079 creator A5087939993 @default.
- W4309657079 date "2022-11-21" @default.
- W4309657079 modified "2023-10-16" @default.
- W4309657079 title "Sensitive Interrogation of Enhancer Activity in Living Cells on a Nanoelectroporation-Probing Platform" @default.
- W4309657079 cites W1796872657 @default.
- W4309657079 cites W1979006359 @default.
- W4309657079 cites W2042660650 @default.
- W4309657079 cites W2102904139 @default.
- W4309657079 cites W2127548079 @default.
- W4309657079 cites W2149748743 @default.
- W4309657079 cites W2151754128 @default.
- W4309657079 cites W2163503448 @default.
- W4309657079 cites W2171111703 @default.
- W4309657079 cites W2295037017 @default.
- W4309657079 cites W2319910816 @default.
- W4309657079 cites W2465336312 @default.
- W4309657079 cites W2521261190 @default.
- W4309657079 cites W2581619747 @default.
- W4309657079 cites W2762129112 @default.
- W4309657079 cites W2769388450 @default.
- W4309657079 cites W2770783660 @default.
- W4309657079 cites W2796366554 @default.
- W4309657079 cites W2799357357 @default.
- W4309657079 cites W2885020841 @default.
- W4309657079 cites W2889051002 @default.
- W4309657079 cites W2895267733 @default.
- W4309657079 cites W2915027573 @default.
- W4309657079 cites W2941157893 @default.
- W4309657079 cites W2944310291 @default.
- W4309657079 cites W2945038365 @default.
- W4309657079 cites W2947097204 @default.
- W4309657079 cites W2950294355 @default.
- W4309657079 cites W2953222982 @default.
- W4309657079 cites W2954495077 @default.
- W4309657079 cites W2970738035 @default.
- W4309657079 cites W2979343233 @default.
- W4309657079 cites W2981217376 @default.
- W4309657079 cites W3007536231 @default.
- W4309657079 cites W3012839541 @default.
- W4309657079 cites W3024613178 @default.
- W4309657079 cites W3028876745 @default.
- W4309657079 cites W3035564435 @default.
- W4309657079 cites W3035889626 @default.
- W4309657079 cites W3042678601 @default.
- W4309657079 cites W3045946041 @default.
- W4309657079 cites W3072127089 @default.
- W4309657079 cites W3084754383 @default.
- W4309657079 cites W3090758913 @default.
- W4309657079 cites W3092927026 @default.
- W4309657079 cites W3108578316 @default.
- W4309657079 cites W3111292947 @default.
- W4309657079 cites W3112346326 @default.
- W4309657079 cites W3113115066 @default.
- W4309657079 cites W3119631020 @default.
- W4309657079 cites W3129615770 @default.
- W4309657079 cites W3147247639 @default.
- W4309657079 cites W3163396627 @default.
- W4309657079 cites W3176042246 @default.
- W4309657079 cites W3185969352 @default.
- W4309657079 cites W3188940990 @default.
- W4309657079 cites W3197597917 @default.
- W4309657079 cites W3207128141 @default.
- W4309657079 cites W4200349790 @default.
- W4309657079 cites W4200351662 @default.
- W4309657079 cites W4214555452 @default.
- W4309657079 cites W4220670146 @default.
- W4309657079 cites W4220718813 @default.
- W4309657079 cites W4223978853 @default.
- W4309657079 cites W4224998087 @default.
- W4309657079 cites W4281295638 @default.
- W4309657079 doi "https://doi.org/10.1021/acssensors.2c01187" @default.
- W4309657079 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36410738" @default.
- W4309657079 hasPublicationYear "2022" @default.
- W4309657079 type Work @default.
- W4309657079 citedByCount "0" @default.
- W4309657079 crossrefType "journal-article" @default.
- W4309657079 hasAuthorship W4309657079A5010734957 @default.
- W4309657079 hasAuthorship W4309657079A5012485751 @default.
- W4309657079 hasAuthorship W4309657079A5014528965 @default.
- W4309657079 hasAuthorship W4309657079A5038830852 @default.
- W4309657079 hasAuthorship W4309657079A5042591538 @default.
- W4309657079 hasAuthorship W4309657079A5054619417 @default.
- W4309657079 hasAuthorship W4309657079A5087939993 @default.
- W4309657079 hasConcept C104317684 @default.
- W4309657079 hasConcept C111936080 @default.
- W4309657079 hasConcept C150194340 @default.
- W4309657079 hasConcept C185592680 @default.
- W4309657079 hasConcept C21784809 @default.
- W4309657079 hasConcept C502942594 @default.
- W4309657079 hasConcept C55493867 @default.