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- W4309657970 abstract "A single fluorescent molecule is highly likely to be located at the center pixel position of a raw image diffused spot in an ideal situation. Even if the molecule and the center pixel position do not completely overlap, they are very close. A single-molecule localization method based on denoising, interpolation and local maxima (DIL) is proposed. The low-resolution raw image is denoised and interpolated, and a new image with a pixel size equal to that of the super-resolution image is attained. The local maxima of the new image are extracted. With this method, it is found that the local maxima positions can be regarded as the fluorescent molecule positions. Simulation results demonstrate that the DIL single-molecule localization accuracy reaches ∼18 nm when the Gaussian noise variance is equal to 0.01. Experimental results demonstrate that the DIL localization methodology is comparable to the Gaussian fitting algorithm and is faster." @default.
- W4309657970 created "2022-11-29" @default.
- W4309657970 creator A5017137755 @default.
- W4309657970 date "2022-11-22" @default.
- W4309657970 modified "2023-09-24" @default.
- W4309657970 title "Single-molecule localization microscopy based on denoising, interpolation and local maxima" @default.
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- W4309657970 doi "https://doi.org/10.1093/jmicro/dfac065" @default.
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