Matches in SemOpenAlex for { <https://semopenalex.org/work/W4309659969> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W4309659969 abstract "Abstract Gene manipulation techniques are fundamental to molecular biology and are continuously being improved. However, gene transfection methods are not established for many unicellular eukaryotes (protists), thereby hindering molecular biological investigations. The oyster parasite Perkinisus marinus is one of the few protists with established gene transfection and drug selection. Nevertheless, the present protocols are tedious, requiring a specific electroporator and pulse conditions which limits the accessibility of this technique across different research groups. Here, we present alternative buffer and electroporation conditions that make the protocol less restrictive. We revealed the pulse condition that enables the introduction of plasmids into P. marinus cell using Ingenio electroporation buffer and NEPA21 electroporator. We found that number of cells and plasmid concentration were critical parameters for the electroporation system. We also constructed a simpler expression plasmid that is removed needless regions for gene expression in the parasite. Our findings resolved the equipment restriction in electroporation of P. marinus and would be a good reference for electroporation in other protists, in particular other Perkinsozoa parasites and core dinoflagellates." @default.
- W4309659969 created "2022-11-29" @default.
- W4309659969 creator A5023894754 @default.
- W4309659969 creator A5038516263 @default.
- W4309659969 creator A5042682367 @default.
- W4309659969 creator A5056530519 @default.
- W4309659969 creator A5068597015 @default.
- W4309659969 creator A5074608513 @default.
- W4309659969 creator A5074955139 @default.
- W4309659969 date "2022-11-21" @default.
- W4309659969 modified "2023-10-17" @default.
- W4309659969 title "Development of a novel electroporation method for the oyster parasite Perkinsus marinus" @default.
- W4309659969 cites W1974316645 @default.
- W4309659969 cites W1979156062 @default.
- W4309659969 cites W1988672718 @default.
- W4309659969 cites W2047290079 @default.
- W4309659969 cites W2118700425 @default.
- W4309659969 cites W2144145338 @default.
- W4309659969 cites W2342160483 @default.
- W4309659969 cites W2563938408 @default.
- W4309659969 cites W2616407115 @default.
- W4309659969 cites W2750343727 @default.
- W4309659969 cites W2892586303 @default.
- W4309659969 cites W2899504596 @default.
- W4309659969 cites W2967373446 @default.
- W4309659969 cites W2967898111 @default.
- W4309659969 cites W3010591673 @default.
- W4309659969 cites W3014987583 @default.
- W4309659969 cites W3152838526 @default.
- W4309659969 cites W3165111765 @default.
- W4309659969 cites W3171351035 @default.
- W4309659969 cites W3189956539 @default.
- W4309659969 cites W3193587014 @default.
- W4309659969 cites W3214807496 @default.
- W4309659969 cites W4205475957 @default.
- W4309659969 cites W4236603600 @default.
- W4309659969 cites W4280615946 @default.
- W4309659969 cites W4293104998 @default.
- W4309659969 doi "https://doi.org/10.1038/s41598-022-24548-0" @default.
- W4309659969 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36411330" @default.
- W4309659969 hasPublicationYear "2022" @default.
- W4309659969 type Work @default.
- W4309659969 citedByCount "1" @default.
- W4309659969 countsByYear W43096599692023 @default.
- W4309659969 crossrefType "journal-article" @default.
- W4309659969 hasAuthorship W4309659969A5023894754 @default.
- W4309659969 hasAuthorship W4309659969A5038516263 @default.
- W4309659969 hasAuthorship W4309659969A5042682367 @default.
- W4309659969 hasAuthorship W4309659969A5056530519 @default.
- W4309659969 hasAuthorship W4309659969A5068597015 @default.
- W4309659969 hasAuthorship W4309659969A5074608513 @default.
- W4309659969 hasAuthorship W4309659969A5074955139 @default.
- W4309659969 hasBestOaLocation W43096599691 @default.
- W4309659969 hasConcept C104317684 @default.
- W4309659969 hasConcept C136764020 @default.
- W4309659969 hasConcept C186852380 @default.
- W4309659969 hasConcept C18903297 @default.
- W4309659969 hasConcept C22744801 @default.
- W4309659969 hasConcept C2776364969 @default.
- W4309659969 hasConcept C41008148 @default.
- W4309659969 hasConcept C54009773 @default.
- W4309659969 hasConcept C54355233 @default.
- W4309659969 hasConcept C70721500 @default.
- W4309659969 hasConcept C71928629 @default.
- W4309659969 hasConcept C86803240 @default.
- W4309659969 hasConcept C95444343 @default.
- W4309659969 hasConceptScore W4309659969C104317684 @default.
- W4309659969 hasConceptScore W4309659969C136764020 @default.
- W4309659969 hasConceptScore W4309659969C186852380 @default.
- W4309659969 hasConceptScore W4309659969C18903297 @default.
- W4309659969 hasConceptScore W4309659969C22744801 @default.
- W4309659969 hasConceptScore W4309659969C2776364969 @default.
- W4309659969 hasConceptScore W4309659969C41008148 @default.
- W4309659969 hasConceptScore W4309659969C54009773 @default.
- W4309659969 hasConceptScore W4309659969C54355233 @default.
- W4309659969 hasConceptScore W4309659969C70721500 @default.
- W4309659969 hasConceptScore W4309659969C71928629 @default.
- W4309659969 hasConceptScore W4309659969C86803240 @default.
- W4309659969 hasConceptScore W4309659969C95444343 @default.
- W4309659969 hasFunder F4320334764 @default.
- W4309659969 hasIssue "1" @default.
- W4309659969 hasLocation W43096599691 @default.
- W4309659969 hasLocation W43096599692 @default.
- W4309659969 hasLocation W43096599693 @default.
- W4309659969 hasOpenAccess W4309659969 @default.
- W4309659969 hasPrimaryLocation W43096599691 @default.
- W4309659969 hasRelatedWork W1979189616 @default.
- W4309659969 hasRelatedWork W2005691771 @default.
- W4309659969 hasRelatedWork W2017021341 @default.
- W4309659969 hasRelatedWork W2037568518 @default.
- W4309659969 hasRelatedWork W2063127271 @default.
- W4309659969 hasRelatedWork W2141284323 @default.
- W4309659969 hasRelatedWork W2336178682 @default.
- W4309659969 hasRelatedWork W2415884740 @default.
- W4309659969 hasRelatedWork W2418965729 @default.
- W4309659969 hasRelatedWork W2428950729 @default.
- W4309659969 hasVolume "12" @default.
- W4309659969 isParatext "false" @default.
- W4309659969 isRetracted "false" @default.
- W4309659969 workType "article" @default.