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- W4309671688 abstract "Abstract Bread wheat (Triticum aestivum L.) is the world’s second-most important cereal crop, as well as India’s. It is an allohexaploid composed of three homeologous sub-genomes (AA, BB, and DD), which is a constraint in determining the complete genome sequence. Several transcription factors have been implicated in both abiotic and biotic stress. WRKY transcription factors are among the best characterised in the context of pathogen defence mechanisms. Different members of the WRKY transcription factors have been shown to confer resistance to stress. But very little is known about the wheat WRKY transcription factors. In silico analysis of the TaWRKY45 protein was performed in the present study using several bioinformatics tools like motif scan, CD search, Netphos, NGlycos, GRAVY, and the SWISS MODEL. The study revealed that TaWRKY45 belongs to the group III family and contains hydrophilic proteins with 19 potential phosphorylation sites. TaWRKY45 protein was found to be orthologous to rice OsWRKY45 by phylogenetic analysis. The catalytic domain was analysed by motif scan which showed that TaWRKY45 has one WRKY domain and a C2-HC zinc finger motif. TaWRKY45’s structure was determined to be more stable, more constrained, more compact, and have greater potential to interact with other molecules than OsWRKY45, according to MD simulation analysis. Thus, in silico analysis of transcription factors helps study protein function, interaction, and regulatory pathways." @default.
- W4309671688 created "2022-11-29" @default.
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- W4309671688 date "2022-11-19" @default.
- W4309671688 modified "2023-09-24" @default.
- W4309671688 title "Structural, functional and evolutionary analysis of wheat WRKY45 protein: A combined bioinformatics and MD simulation approach" @default.
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- W4309671688 doi "https://doi.org/10.1101/2022.11.18.517088" @default.
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