Matches in SemOpenAlex for { <https://semopenalex.org/work/W4309674007> ?p ?o ?g. }
- W4309674007 endingPage "22139" @default.
- W4309674007 startingPage "22128" @default.
- W4309674007 abstract "Proteins can adopt different conformational states that are important for their biological function and, in some cases, can be responsible for their dysfunction. The essential roles that proteins play in biological systems make distinguishing the structural differences between these conformational states both fundamentally and practically important. Here, we demonstrate that collision-induced unfolding (CIU), in combination with ion mobility–mass spectrometry (IM–MS) measurements, distinguish subtly different conformational states for protein complexes. Using the open and closed states of the β-lactoglobulin (βLG) dimer as a model, we show that these two conformational isomers unfold during collisional activation to generate distinct states that are readily separated by IM–MS. Extensive molecular modeling of the CIU process reproduces the distinct unfolding intermediates and identifies the molecular details that explain why the two conformational states unfold in distinct ways. Strikingly, the open conformational state forms new electrostatic interactions upon collisional heating, while the closed state does not. These newly formed electrostatic interactions involve residues on the loop differentially positioned in the two βLG conformational isomers, highlighting that gas-phase unfolding pathways reflect aspects of solution structure. This combination of experiment and theory provides a path forward for distinguishing subtly different conformational isomers for protein complexes via gas-phase unfolding experiments. Our results also have implications for understanding how protein complexes dissociate in the gas phase, indicating that current models need to be refined to explain protein complex dissociation." @default.
- W4309674007 created "2022-11-29" @default.
- W4309674007 creator A5011027339 @default.
- W4309674007 creator A5070876073 @default.
- W4309674007 date "2022-11-22" @default.
- W4309674007 modified "2023-10-17" @default.
- W4309674007 title "Gas-Phase Unfolding of Protein Complexes Distinguishes Conformational Isomers" @default.
- W4309674007 cites W1254760643 @default.
- W4309674007 cites W1980705414 @default.
- W4309674007 cites W1987505759 @default.
- W4309674007 cites W1991832340 @default.
- W4309674007 cites W1992945955 @default.
- W4309674007 cites W2000830335 @default.
- W4309674007 cites W2002257505 @default.
- W4309674007 cites W2004061506 @default.
- W4309674007 cites W2005919833 @default.
- W4309674007 cites W2006910241 @default.
- W4309674007 cites W2007968374 @default.
- W4309674007 cites W2011887852 @default.
- W4309674007 cites W2013302888 @default.
- W4309674007 cites W2016589902 @default.
- W4309674007 cites W2023781499 @default.
- W4309674007 cites W2028401722 @default.
- W4309674007 cites W2030265676 @default.
- W4309674007 cites W2031674548 @default.
- W4309674007 cites W2035314417 @default.
- W4309674007 cites W2035546135 @default.
- W4309674007 cites W2039125944 @default.
- W4309674007 cites W2041516405 @default.
- W4309674007 cites W2054881399 @default.
- W4309674007 cites W2082518459 @default.
- W4309674007 cites W2088144123 @default.
- W4309674007 cites W2090615391 @default.
- W4309674007 cites W2110130604 @default.
- W4309674007 cites W2147988069 @default.
- W4309674007 cites W2154019529 @default.
- W4309674007 cites W2161174294 @default.
- W4309674007 cites W2166185900 @default.
- W4309674007 cites W2170460752 @default.
- W4309674007 cites W2305853196 @default.
- W4309674007 cites W2320420686 @default.
- W4309674007 cites W2321675547 @default.
- W4309674007 cites W2404331511 @default.
- W4309674007 cites W2408391104 @default.
- W4309674007 cites W2471933976 @default.
- W4309674007 cites W2506865782 @default.
- W4309674007 cites W2523295835 @default.
- W4309674007 cites W2567022102 @default.
- W4309674007 cites W2588017397 @default.
- W4309674007 cites W2611442133 @default.
- W4309674007 cites W2614053601 @default.
- W4309674007 cites W2732158985 @default.
- W4309674007 cites W2744913579 @default.
- W4309674007 cites W2772936471 @default.
- W4309674007 cites W2784728449 @default.
- W4309674007 cites W2802015630 @default.
- W4309674007 cites W2886952883 @default.
- W4309674007 cites W2890980244 @default.
- W4309674007 cites W2913431089 @default.
- W4309674007 cites W2919424795 @default.
- W4309674007 cites W2942966726 @default.
- W4309674007 cites W2947791494 @default.
- W4309674007 cites W2956492594 @default.
- W4309674007 cites W2970509915 @default.
- W4309674007 cites W2973060072 @default.
- W4309674007 cites W2985777491 @default.
- W4309674007 cites W2994782608 @default.
- W4309674007 cites W3129026034 @default.
- W4309674007 cites W3130337837 @default.
- W4309674007 cites W3153087837 @default.
- W4309674007 cites W3157814829 @default.
- W4309674007 cites W3185197558 @default.
- W4309674007 cites W3196727208 @default.
- W4309674007 cites W4207068619 @default.
- W4309674007 cites W4248888255 @default.
- W4309674007 doi "https://doi.org/10.1021/jacs.2c09573" @default.
- W4309674007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36414315" @default.
- W4309674007 hasPublicationYear "2022" @default.
- W4309674007 type Work @default.
- W4309674007 citedByCount "4" @default.
- W4309674007 countsByYear W43096740072022 @default.
- W4309674007 countsByYear W43096740072023 @default.
- W4309674007 crossrefType "journal-article" @default.
- W4309674007 hasAuthorship W4309674007A5011027339 @default.
- W4309674007 hasAuthorship W4309674007A5070876073 @default.
- W4309674007 hasConcept C102931765 @default.
- W4309674007 hasConcept C147597530 @default.
- W4309674007 hasConcept C147789679 @default.
- W4309674007 hasConcept C159467904 @default.
- W4309674007 hasConcept C178790620 @default.
- W4309674007 hasConcept C185592680 @default.
- W4309674007 hasConcept C18705241 @default.
- W4309674007 hasConcept C2777339483 @default.
- W4309674007 hasConcept C2779546866 @default.
- W4309674007 hasConcept C3020013744 @default.
- W4309674007 hasConcept C32909587 @default.
- W4309674007 hasConcept C47701112 @default.
- W4309674007 hasConcept C55493867 @default.