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- W4309698583 abstract "The movement trajectories of organisms serve as dynamic read-outs of their behaviour and physiology. For microorganisms this can be difficult to resolve due to their small size and fast movement. Here, we devise a novel droplet microfluidics assay to encapsulate single micron-sized algae inside closed arenas, enabling ultralong high-speed tracking of the same cell. Comparing two model species - Chlamydomonas reinhardtii (freshwater, 2 cilia), and Pyramimonas octopus (marine, 8 cilia), we detail their highly-stereotyped yet contrasting swimming behaviours and environmental interactions. By measuring the rates and probabilities with which cells transition between a trio of motility states (smooth-forward swimming, quiescence, tumbling or excitable backward swimming), we reconstruct the control network that underlies this gait switching dynamics. A simplified model of cell-roaming in circular confinement reproduces the observed long-term behaviours and spatial fluxes, including novel boundary circulation behaviour. Finally, we establish an assay in which pairs of droplets are fused on demand, one containing a trapped cell with another containing a chemical that perturbs cellular excitability, to reveal how aneural microorganisms adapt their locomotor patterns in real-time." @default.
- W4309698583 created "2022-11-29" @default.
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- W4309698583 date "2022-11-23" @default.
- W4309698583 modified "2023-09-25" @default.
- W4309698583 title "Phenotyping single-cell motility in microfluidic confinement" @default.
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- W4309698583 doi "https://doi.org/10.7554/elife.76519" @default.
- W4309698583 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36416411" @default.
- W4309698583 hasPublicationYear "2022" @default.
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