FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4309740020> ?p ?o ?g. }

• W4309740020 abstract "The key enzyme 6-phosphofructo-2-kinase (PFK2)/fructose-2,6-bisphosphatase (FBPase-2) is responsible for regulating the rates of glycolysis and gluconeogenesis in eukaryotes. However, its functions and mechanisms in filamentous fungi remain largely enigmatic. In this study, we systematically investigated the function of this enzyme in Myceliophthora thermophila , a thermophilic filamentous fungus with great capacity to produce industrial enzymes and organic acids. Our results showed that the M. thermophila genome encodes three isomers, all with the PFK2/FBPase-2 structure: pfk2-a, pfk2-b , and pfk2-c . Overexpression of each gene revealed that endogenous expression of pfk2-c (PFK2 activity) promoted glucose metabolism, while overexpression of pfk2-a (FBPase-2 activity) inhibited strain growth. Using knockouts, we found that each gene was individually non-essential, but the triple knockout led to significantly slower growth compared with the wild-type strain. Only the pfk2-a single knockout exhibited 22.15% faster sugar metabolism, exerted through activation of 6-phosphofructo-1-kinase (PFK1), thereby significantly promoting glycolysis and the tricarboxylic acid cycle. The FBPase-2 deletion mutant strain also exhibited overflow metabolism, and knocking out pfk2-a was proved to be able to improve the production and synthesis rate of various metabolites, such as glycerol and malate. This is the first study to systematically investigate the function of PFK2/FBPase-2 in a thermophilic fungus, providing an effective target for metabolic engineering in filamentous fungi." @default.
• W4309740020 created "2022-11-29" @default.
• W4309740020 creator A5005359731 @default.
• W4309740020 creator A5039360592 @default.
• W4309740020 creator A5039467147 @default.
• W4309740020 creator A5062290117 @default.
• W4309740020 creator A5084461937 @default.
• W4309740020 date "2022-11-21" @default.
• W4309740020 modified "2023-10-02" @default.
• W4309740020 title "PFK2/FBPase-2 is a potential target for metabolic engineering in the filamentous fungus Myceliophthora thermophila" @default.
• W4309740020 cites W1511847298 @default.
• W4309740020 cites W1537741204 @default.
• W4309740020 cites W1852807937 @default.
• W4309740020 cites W1966761605 @default.
• W4309740020 cites W1967481307 @default.
• W4309740020 cites W1990133090 @default.
• W4309740020 cites W1991461352 @default.
• W4309740020 cites W2000081372 @default.
• W4309740020 cites W2009152604 @default.
• W4309740020 cites W2011241671 @default.
• W4309740020 cites W2047578327 @default.
• W4309740020 cites W2058992335 @default.
• W4309740020 cites W2070669901 @default.
• W4309740020 cites W2077055748 @default.
• W4309740020 cites W2086119026 @default.
• W4309740020 cites W2099941694 @default.
• W4309740020 cites W2106223802 @default.
• W4309740020 cites W2107277218 @default.
• W4309740020 cites W2119787018 @default.
• W4309740020 cites W2127862649 @default.
• W4309740020 cites W2133836136 @default.
• W4309740020 cites W2137059405 @default.
• W4309740020 cites W2157179660 @default.
• W4309740020 cites W2169733090 @default.
• W4309740020 cites W2192080449 @default.
• W4309740020 cites W2551418054 @default.
• W4309740020 cites W2561030438 @default.
• W4309740020 cites W2804905636 @default.
• W4309740020 cites W2810661705 @default.
• W4309740020 cites W2888301535 @default.
• W4309740020 cites W2900522561 @default.
• W4309740020 cites W2938031627 @default.
• W4309740020 cites W2943978406 @default.
• W4309740020 cites W3011379747 @default.
• W4309740020 cites W3013169660 @default.
• W4309740020 cites W4213268335 @default.
• W4309740020 cites W4237179979 @default.
• W4309740020 cites W4286247382 @default.
• W4309740020 cites W6588457 @default.
• W4309740020 cites W927341241 @default.
• W4309740020 doi "https://doi.org/10.3389/fmicb.2022.1056694" @default.
• W4309740020 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36478865" @default.
• W4309740020 hasPublicationYear "2022" @default.
• W4309740020 type Work @default.
• W4309740020 citedByCount "1" @default.
• W4309740020 countsByYear W43097400202023 @default.
• W4309740020 crossrefType "journal-article" @default.
• W4309740020 hasAuthorship W4309740020A5005359731 @default.
• W4309740020 hasAuthorship W4309740020A5039360592 @default.
• W4309740020 hasAuthorship W4309740020A5039467147 @default.
• W4309740020 hasAuthorship W4309740020A5062290117 @default.
• W4309740020 hasAuthorship W4309740020A5084461937 @default.
• W4309740020 hasBestOaLocation W43097400201 @default.
• W4309740020 hasConcept C104317684 @default.
• W4309740020 hasConcept C143065580 @default.
• W4309740020 hasConcept C165135838 @default.
• W4309740020 hasConcept C181199279 @default.
• W4309740020 hasConcept C184235292 @default.
• W4309740020 hasConcept C189909181 @default.
• W4309740020 hasConcept C20251656 @default.
• W4309740020 hasConcept C2776970464 @default.
• W4309740020 hasConcept C2777576037 @default.
• W4309740020 hasConcept C27911776 @default.
• W4309740020 hasConcept C55493867 @default.
• W4309740020 hasConcept C6350086 @default.
• W4309740020 hasConcept C77957584 @default.
• W4309740020 hasConcept C86803240 @default.
• W4309740020 hasConceptScore W4309740020C104317684 @default.
• W4309740020 hasConceptScore W4309740020C143065580 @default.
• W4309740020 hasConceptScore W4309740020C165135838 @default.
• W4309740020 hasConceptScore W4309740020C181199279 @default.
• W4309740020 hasConceptScore W4309740020C184235292 @default.
• W4309740020 hasConceptScore W4309740020C189909181 @default.
• W4309740020 hasConceptScore W4309740020C20251656 @default.
• W4309740020 hasConceptScore W4309740020C2776970464 @default.
• W4309740020 hasConceptScore W4309740020C2777576037 @default.
• W4309740020 hasConceptScore W4309740020C27911776 @default.
• W4309740020 hasConceptScore W4309740020C55493867 @default.
• W4309740020 hasConceptScore W4309740020C6350086 @default.
• W4309740020 hasConceptScore W4309740020C77957584 @default.
• W4309740020 hasConceptScore W4309740020C86803240 @default.
• W4309740020 hasLocation W43097400201 @default.
• W4309740020 hasLocation W43097400202 @default.
• W4309740020 hasLocation W43097400203 @default.
• W4309740020 hasOpenAccess W4309740020 @default.
• W4309740020 hasPrimaryLocation W43097400201 @default.
• W4309740020 hasRelatedWork W2004231513 @default.
• W4309740020 hasRelatedWork W2008102720 @default.
• W4309740020 hasRelatedWork W2010795601 @default.
• W4309740020 hasRelatedWork W2035682770 @default.

• next