Matches in SemOpenAlex for { <https://semopenalex.org/work/W4309746231> ?p ?o ?g. }
- W4309746231 abstract "Culture environment during in vitro embryo production can affect embryo phenotype and pregnancy outcomes, making culture modifications a logical approach for improving embryo competence. Previously, the addition of the growth factors FGF2, LIF, and IGF1, termed FLI, to the culture medium improved bovine embryo development, and re-expansion following cryopreservation. The objective of this study was to investigate the survival of cryopreserved FLI treated embryos at day 15 of pregnancy and evaluate conceptus transcriptomes. Embryos were produced using in vitro fertilization of abattoir-derived oocytes, cultured to the blastocyst stage in the presence or absence of FLI (+/- FLI), and cryopreserved by slow-rate freezing. Thawed embryos were transferred into non-lactating recipient beef cows and eight days later conceptuses were recovered and analyzed. For a subset of conceptuses whole transcriptome analysis was performed by using the NovaSeq 6000. There was no detectable difference in conceptus recovery or average conceptus length between the two groups. There were 32 differentially expressed transcripts, 23 up-regulated and nine down-regulated in the +FLI group compared to -FLI. Genes were involved in interferon signaling, prostaglandin synthesis, and placental development. This study reveals that embryos cultured with or without FLI and cryopreserved by slow-rate freezing have similar developmental competence up to day 15 of development. Nevertheless, differences in gene expression exhibit an effect of FLI on conceptus signaling during elongation." @default.
- W4309746231 created "2022-11-29" @default.
- W4309746231 creator A5004859455 @default.
- W4309746231 creator A5010157169 @default.
- W4309746231 creator A5050988587 @default.
- W4309746231 creator A5066185112 @default.
- W4309746231 creator A5069001249 @default.
- W4309746231 date "2022-11-21" @default.
- W4309746231 modified "2023-10-18" @default.
- W4309746231 title "Actions of FGF2, LIF, and IGF1 on bovine embryo survival and conceptus elongation following slow-rate freezing" @default.
- W4309746231 cites W1518576897 @default.
- W4309746231 cites W1966973271 @default.
- W4309746231 cites W1967108419 @default.
- W4309746231 cites W1975506676 @default.
- W4309746231 cites W1976190649 @default.
- W4309746231 cites W1996016258 @default.
- W4309746231 cites W2004465286 @default.
- W4309746231 cites W2004558200 @default.
- W4309746231 cites W2011899256 @default.
- W4309746231 cites W2015683661 @default.
- W4309746231 cites W2019753523 @default.
- W4309746231 cites W2033641220 @default.
- W4309746231 cites W2041470264 @default.
- W4309746231 cites W2042912474 @default.
- W4309746231 cites W2043582769 @default.
- W4309746231 cites W2048307484 @default.
- W4309746231 cites W2063156156 @default.
- W4309746231 cites W2069501768 @default.
- W4309746231 cites W2082173249 @default.
- W4309746231 cites W2095701213 @default.
- W4309746231 cites W2098060716 @default.
- W4309746231 cites W2098150099 @default.
- W4309746231 cites W2102278945 @default.
- W4309746231 cites W2110752569 @default.
- W4309746231 cites W2112392835 @default.
- W4309746231 cites W2119598306 @default.
- W4309746231 cites W2122119863 @default.
- W4309746231 cites W2122711888 @default.
- W4309746231 cites W2127359614 @default.
- W4309746231 cites W2127542959 @default.
- W4309746231 cites W2134689524 @default.
- W4309746231 cites W2138207763 @default.
- W4309746231 cites W2141658598 @default.
- W4309746231 cites W2142879707 @default.
- W4309746231 cites W2153875029 @default.
- W4309746231 cites W2164278911 @default.
- W4309746231 cites W2164387992 @default.
- W4309746231 cites W2171359207 @default.
- W4309746231 cites W2179602430 @default.
- W4309746231 cites W2220682555 @default.
- W4309746231 cites W2223318443 @default.
- W4309746231 cites W2268471462 @default.
- W4309746231 cites W2293274576 @default.
- W4309746231 cites W2308008356 @default.
- W4309746231 cites W2337665072 @default.
- W4309746231 cites W2579795012 @default.
- W4309746231 cites W2725322436 @default.
- W4309746231 cites W2758057470 @default.
- W4309746231 cites W2809421634 @default.
- W4309746231 cites W2892444606 @default.
- W4309746231 cites W2900337332 @default.
- W4309746231 cites W2937880699 @default.
- W4309746231 cites W2938690325 @default.
- W4309746231 cites W2944109291 @default.
- W4309746231 cites W2952901868 @default.
- W4309746231 cites W2991622560 @default.
- W4309746231 cites W2997022986 @default.
- W4309746231 cites W3009650058 @default.
- W4309746231 cites W3095471805 @default.
- W4309746231 cites W3127066098 @default.
- W4309746231 cites W3159098484 @default.
- W4309746231 cites W394076291 @default.
- W4309746231 doi "https://doi.org/10.3389/fanim.2022.1040064" @default.
- W4309746231 hasPublicationYear "2022" @default.
- W4309746231 type Work @default.
- W4309746231 citedByCount "1" @default.
- W4309746231 countsByYear W43097462312023 @default.
- W4309746231 crossrefType "journal-article" @default.
- W4309746231 hasAuthorship W4309746231A5004859455 @default.
- W4309746231 hasAuthorship W4309746231A5010157169 @default.
- W4309746231 hasAuthorship W4309746231A5050988587 @default.
- W4309746231 hasAuthorship W4309746231A5066185112 @default.
- W4309746231 hasAuthorship W4309746231A5069001249 @default.
- W4309746231 hasBestOaLocation W43097462311 @default.
- W4309746231 hasConcept C104317684 @default.
- W4309746231 hasConcept C150194340 @default.
- W4309746231 hasConcept C162317418 @default.
- W4309746231 hasConcept C16685009 @default.
- W4309746231 hasConcept C172680121 @default.
- W4309746231 hasConcept C179933525 @default.
- W4309746231 hasConcept C196843134 @default.
- W4309746231 hasConcept C2778177303 @default.
- W4309746231 hasConcept C2779234561 @default.
- W4309746231 hasConcept C2779335785 @default.
- W4309746231 hasConcept C32253518 @default.
- W4309746231 hasConcept C54355233 @default.
- W4309746231 hasConcept C71924100 @default.
- W4309746231 hasConcept C86803240 @default.
- W4309746231 hasConcept C87073359 @default.
- W4309746231 hasConcept C95444343 @default.