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- W4309990481 abstract "The role of a soybean 14-3-3 gene (Glyma05g29080) in defense against white mold and in nodulation was investigated by loss-of-gene-function with CRISPR-Cas9 editing and silencing of RNA interference (RNAi). Particle bombardment was used to introduce the CRISPR expression cassette to target the soybean 14-3-3 gene and an RNAi construct to silence gene transcription. Transmission of the edited 14-3-3 gene and the RNAi construct was confirmed in their respective progeny. The recovered transgenic plants and their progeny were significantly more susceptible to Sclerotinia sclerotiorum infection and showed a significant reduction in nodulation, thus confirming the role of the 14-3-3 gene (Glyma05g29080) in both nodulation and defense." @default.
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- W4309990481 date "2023-03-01" @default.
- W4309990481 modified "2023-10-14" @default.
- W4309990481 title "The Role of a Soybean 14-3-3 Gene (<i>Glyma05g29080</i>) on White Mold Resistance and Nodulation Investigations Using CRISPR-Cas9 Editing and RNA Silencing" @default.
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- W4309990481 doi "https://doi.org/10.1094/mpmi-07-22-0157-r" @default.
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