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- W4309995346 abstract "Abstract Known as the powerhouses of cells, mitochondria and its dynamics are important for their functions in cells. Herein, an optogenetic method that controlling mitochondria to form the clusters was developed. The plasmid named CRY2PHR-mCherry-Miro1TM was designed for the optogenetic system. The photoactivable protein CRY2PHR was anchored to mitochondria, via the specific organelle-targeting transmembrane domain Miro1TM. Under blue light illumination, CRY2PHR can form the oligomerization, called puncta. With the illuminated time extended, the puncta can interact, and the mitochondria were found to form clustering with reversibility and spatiotemporal controllability. The mitochondrial functions were found to enhance after the formation of optogenetic mitochondrial clusters. This method presented here provides a way to control mitochondrial clustering and raise mitochondrial functions up." @default.
- W4309995346 created "2022-11-30" @default.
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- W4309995346 date "2022-11-23" @default.
- W4309995346 modified "2023-10-18" @default.
- W4309995346 title "Enhancing Mitochondrial Functions by Optogenetic Clustering" @default.
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- W4309995346 doi "https://doi.org/10.1101/2022.11.22.517578" @default.
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