FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4310125627> ?p ?o ?g. }

• W4310125627 endingPage "11853" @default.
• W4310125627 startingPage "11852" @default.
• W4310125627 abstract "Introduction: Heat shock proteins (HSPs) are ancient and highly conserved genes that participate in protein quality control in eukaryotes and bacteria. These proteins are produced in large quantities when cells are exposed to stress that causes denaturation of essential proteins. HSPs help maintain cellular homeostasis, and act as molecular chaperones, assisting with protein folding and stability, multi-protein assembly, intracellular protein trafficking and degrading damaged proteins. The Hsp90 family of proteins includes 4 genes, with 1 (HtpG) being a bacterial form. Members of the Hsp90 family interact with a diverse set of client proteins, some involved in cell signalling and proliferation. Many of these client proteins are involved in growth, survival and adaptation of cancer cells. Eukaryotic Hsp90 plays an important role in tumorigenesis and there has been much research into the use of Hsp90 inhibitors to inhibit growth of cancer cells, but little has been done to examine what role the other paralogues have in stabilizing cancer cells. In addition, no work to date has looked at whether bacterial HtpG paralogues might interact with cancer cells. While it is anticipated that bacterial stress and the behaviour of HtpG would correlate to the host response to stress, this has not been examined. The first step is to examine bacterial HtpG gene levels before and during induction to determine if there are changes in HtpG gene abundance. Methods: Forty-eight stool samples were collected from 29 pediatric patients with Acute Lymphoblastic Leukemia (ALL) being treated at the IWK health centre, Nova Scotia, Canada after informed consent. DNA was extracted and whole shotgun metagenome sequencing (Illumina, Nextera XT) was performed. Low quality and contaminated sequences (human and PhiX174) were removed using the kneaddata pipeline (Trimmomatic and Bowtie2). Paired cleaned sequences were concatenated and HUMAnN3 was used to assign gene families to reads using the Uniref90 database. Genes assigned to HtpG or Hsp90-like gene families were included and summed from each sample and corrected for sequencing depth. Samples were grouped by phase of treatment (13 pre-treatment samples from 13 patients and 35 induction samples from 23 patients) for analysis. Comparisons between pre-treatment (PT) and induction (IN) samples were performed using Wilcoxon-Mann-Whitney U. Fisher's exact test was used to compare age group and sex between PT and IN samples. The study was approved by the IWK ethics board. Results: Neither sex nor age category differed between PT and IN (p=1). HtpG gene counts among the 48 stool samples from 29 patients ranged from 0 to 329 (mean 119). The mean counts of HtpG genes were significantly decreased in samples during induction therapy compared to pre-treatment (p=0.002). Shannon diversity, a measure of microbial diversity, did not differ between PT and IN samples (p=0.2793), nor did counts of bacteria (Chao-1 p=0.47). Discussion: Studies have found that Hsp90 is generally over expressed in cancer patients with elevated levels, suggesting poorer overall survival. They have been proposed as a potential biomarker of poor prognosis. We had anticipated increased stress and protein damage to occur in the gut environment as a result of chemotherapy, rather than as a result of the underlying cancer. The anticipated increase in HtpG during induction did not occur but rather declined with cancer remission. One possibility is that the decline in HtpG genes was a result of a loss of bacterial diversity due to treatment. However, we did not see significant differences in diversity between PT and IN. The increase in HtpG genes during PT and reduction during induction therapy seems to follow a similar pattern to Hsp90 expression in host cells, suggesting that bacterial HtpG might also serve as a marker of disease remission. It also highlights that a better understanding of bacterial HtpG, a protein known to be present in outer membrane vesicles, which are crucial for host communication and have been implicated in contributing to carcinogenic potential is warranted. Conclusion: This study suggests that the bacterial chaperone HtpG is possibly related to degree of inflammation caused by leukemic cells rather than leukemia treatment. A larger study is needed to verify the potential of HtpG as a marker of cancer remission or treatment toxicity or cancer immune response." @default.
• W4310125627 created "2022-11-30" @default.
• W4310125627 creator A5032969907 @default.
• W4310125627 creator A5037244921 @default.
• W4310125627 creator A5041896044 @default.
• W4310125627 creator A5049504526 @default.
• W4310125627 creator A5062243997 @default.
• W4310125627 creator A5067693548 @default.
• W4310125627 date "2022-11-15" @default.
• W4310125627 modified "2023-10-01" @default.
• W4310125627 title "The Bacterial Chaperone High-Temperature Protein G (HtpG) Decreases in Abundance with Induction Therapy in Children with Acute Lymphoblastic Leukemia" @default.
• W4310125627 doi "https://doi.org/10.1182/blood-2022-163538" @default.
• W4310125627 hasPublicationYear "2022" @default.
• W4310125627 type Work @default.
• W4310125627 citedByCount "1" @default.
• W4310125627 countsByYear W43101256272023 @default.
• W4310125627 crossrefType "journal-article" @default.
• W4310125627 hasAuthorship W4310125627A5032969907 @default.
• W4310125627 hasAuthorship W4310125627A5037244921 @default.
• W4310125627 hasAuthorship W4310125627A5041896044 @default.
• W4310125627 hasAuthorship W4310125627A5049504526 @default.
• W4310125627 hasAuthorship W4310125627A5062243997 @default.
• W4310125627 hasAuthorship W4310125627A5067693548 @default.
• W4310125627 hasConcept C126322002 @default.
• W4310125627 hasConcept C142724271 @default.
• W4310125627 hasConcept C203014093 @default.
• W4310125627 hasConcept C2775962898 @default.
• W4310125627 hasConcept C2776694085 @default.
• W4310125627 hasConcept C2778461978 @default.
• W4310125627 hasConcept C2781107101 @default.
• W4310125627 hasConcept C2909962599 @default.
• W4310125627 hasConcept C2991767035 @default.
• W4310125627 hasConcept C2993175405 @default.
• W4310125627 hasConcept C502942594 @default.
• W4310125627 hasConcept C523546767 @default.
• W4310125627 hasConcept C54355233 @default.
• W4310125627 hasConcept C71924100 @default.
• W4310125627 hasConcept C86803240 @default.
• W4310125627 hasConceptScore W4310125627C126322002 @default.
• W4310125627 hasConceptScore W4310125627C142724271 @default.
• W4310125627 hasConceptScore W4310125627C203014093 @default.
• W4310125627 hasConceptScore W4310125627C2775962898 @default.
• W4310125627 hasConceptScore W4310125627C2776694085 @default.
• W4310125627 hasConceptScore W4310125627C2778461978 @default.
• W4310125627 hasConceptScore W4310125627C2781107101 @default.
• W4310125627 hasConceptScore W4310125627C2909962599 @default.
• W4310125627 hasConceptScore W4310125627C2991767035 @default.
• W4310125627 hasConceptScore W4310125627C2993175405 @default.
• W4310125627 hasConceptScore W4310125627C502942594 @default.
• W4310125627 hasConceptScore W4310125627C523546767 @default.
• W4310125627 hasConceptScore W4310125627C54355233 @default.
• W4310125627 hasConceptScore W4310125627C71924100 @default.
• W4310125627 hasConceptScore W4310125627C86803240 @default.
• W4310125627 hasIssue "Supplement 1" @default.
• W4310125627 hasLocation W43101256271 @default.
• W4310125627 hasOpenAccess W4310125627 @default.
• W4310125627 hasPrimaryLocation W43101256271 @default.
• W4310125627 hasRelatedWork W1984143857 @default.
• W4310125627 hasRelatedWork W2187120518 @default.
• W4310125627 hasRelatedWork W2339313676 @default.
• W4310125627 hasRelatedWork W2417244325 @default.
• W4310125627 hasRelatedWork W2419219125 @default.
• W4310125627 hasRelatedWork W2428449797 @default.
• W4310125627 hasRelatedWork W2770032321 @default.
• W4310125627 hasRelatedWork W3030676104 @default.
• W4310125627 hasRelatedWork W373319614 @default.
• W4310125627 hasRelatedWork W4310125627 @default.
• W4310125627 hasVolume "140" @default.
• W4310125627 isParatext "false" @default.
• W4310125627 isRetracted "false" @default.
• W4310125627 workType "article" @default.