Matches in SemOpenAlex for { <https://semopenalex.org/work/W4310241418> ?p ?o ?g. }
- W4310241418 abstract "Abstract Canine babesiosis is a tick-borne disease caused by Babesia spp., which infects and destroys healthy erythrocytes, leading to mortality and morbidity in dogs. The diagnosis of babesiosis is tedious and time-consuming, especially in latent and chronic infections. Here, a recombinase polymerase amplification combined with a lateral flow dipstick (RPA-LFD) assay was developed for rapid and accurate detection of Babesia spp. in canine blood specimens based on the 18S rRNA region. The RPA-LFD assay using rpaBab264 gave specificity to Babesia spp. in dogs ( B. vogeli and B. gibsoni ) without cross-amplification to other parasites (apicomplexans and non-apicomplexans), with detection limit of at least 22.5 copies/μl (0.1 fg/µl) at 40 °C for at least 10 min. The whole process of DNA amplification by RPA and readout by LFD did not exceed 30 min. To determine the performance of the RPA-LFD assay, a total of 30 clinical samples was examined and compared with conventional PCR (cPCR) and multiplex HRM (mHRM). Eight dogs (26.67%) were detected as positive by RPA-LFD, while seven and six were found positive by cPCR and mHRM, respectively. RPA-LFD and cPCR showed high agreement with Babesia spp. detection with kappa > 0.9. We confirmed that the dogs were infected by B. vogeli from sequences of positive PCR results. Our findings suggested that RPA-LFD using the rpaBab264 assay offered a rapid, accurate, cost-effective and simple method for Babesia spp. detection that is feasibly applicable to be rapid kit at a pet hospital or point-of-care testing." @default.
- W4310241418 created "2022-11-30" @default.
- W4310241418 creator A5009455195 @default.
- W4310241418 creator A5015945178 @default.
- W4310241418 creator A5018658641 @default.
- W4310241418 creator A5021829119 @default.
- W4310241418 creator A5021952768 @default.
- W4310241418 creator A5041466584 @default.
- W4310241418 creator A5041621318 @default.
- W4310241418 creator A5073194015 @default.
- W4310241418 creator A5086105454 @default.
- W4310241418 date "2022-11-29" @default.
- W4310241418 modified "2023-09-25" @default.
- W4310241418 title "Sensitive and rapid detection of Babesia species in dogs by recombinase polymerase amplification with lateral flow dipstick (RPA-LFD)" @default.
- W4310241418 cites W1992682141 @default.
- W4310241418 cites W2000230397 @default.
- W4310241418 cites W2025073661 @default.
- W4310241418 cites W2040369217 @default.
- W4310241418 cites W2041702643 @default.
- W4310241418 cites W2059597738 @default.
- W4310241418 cites W2081943100 @default.
- W4310241418 cites W2094509729 @default.
- W4310241418 cites W2115356485 @default.
- W4310241418 cites W2137913153 @default.
- W4310241418 cites W2153350094 @default.
- W4310241418 cites W2155358648 @default.
- W4310241418 cites W2168499180 @default.
- W4310241418 cites W2177562804 @default.
- W4310241418 cites W2179656053 @default.
- W4310241418 cites W2188273241 @default.
- W4310241418 cites W2379742646 @default.
- W4310241418 cites W2415826107 @default.
- W4310241418 cites W2418163421 @default.
- W4310241418 cites W2605783353 @default.
- W4310241418 cites W2759665919 @default.
- W4310241418 cites W2766989075 @default.
- W4310241418 cites W2780981686 @default.
- W4310241418 cites W2793266470 @default.
- W4310241418 cites W2800905921 @default.
- W4310241418 cites W2895446304 @default.
- W4310241418 cites W2912574281 @default.
- W4310241418 cites W2920871162 @default.
- W4310241418 cites W2945256932 @default.
- W4310241418 cites W2967210422 @default.
- W4310241418 cites W2991640646 @default.
- W4310241418 cites W2996758853 @default.
- W4310241418 cites W3006856816 @default.
- W4310241418 cites W3158464861 @default.
- W4310241418 cites W3159382670 @default.
- W4310241418 cites W3160959111 @default.
- W4310241418 cites W3216198145 @default.
- W4310241418 cites W4292218227 @default.
- W4310241418 cites W4300816714 @default.
- W4310241418 doi "https://doi.org/10.1038/s41598-022-25165-7" @default.
- W4310241418 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36446883" @default.
- W4310241418 hasPublicationYear "2022" @default.
- W4310241418 type Work @default.
- W4310241418 citedByCount "1" @default.
- W4310241418 countsByYear W43102414182023 @default.
- W4310241418 crossrefType "journal-article" @default.
- W4310241418 hasAuthorship W4310241418A5009455195 @default.
- W4310241418 hasAuthorship W4310241418A5015945178 @default.
- W4310241418 hasAuthorship W4310241418A5018658641 @default.
- W4310241418 hasAuthorship W4310241418A5021829119 @default.
- W4310241418 hasAuthorship W4310241418A5021952768 @default.
- W4310241418 hasAuthorship W4310241418A5041466584 @default.
- W4310241418 hasAuthorship W4310241418A5041621318 @default.
- W4310241418 hasAuthorship W4310241418A5073194015 @default.
- W4310241418 hasAuthorship W4310241418A5086105454 @default.
- W4310241418 hasBestOaLocation W43102414181 @default.
- W4310241418 hasConcept C104317684 @default.
- W4310241418 hasConcept C139713532 @default.
- W4310241418 hasConcept C153911025 @default.
- W4310241418 hasConcept C159047783 @default.
- W4310241418 hasConcept C2777119855 @default.
- W4310241418 hasConcept C2778145968 @default.
- W4310241418 hasConcept C2780026642 @default.
- W4310241418 hasConcept C2780327821 @default.
- W4310241418 hasConcept C2780581805 @default.
- W4310241418 hasConcept C49105822 @default.
- W4310241418 hasConcept C54355233 @default.
- W4310241418 hasConcept C55493867 @default.
- W4310241418 hasConcept C86803240 @default.
- W4310241418 hasConceptScore W4310241418C104317684 @default.
- W4310241418 hasConceptScore W4310241418C139713532 @default.
- W4310241418 hasConceptScore W4310241418C153911025 @default.
- W4310241418 hasConceptScore W4310241418C159047783 @default.
- W4310241418 hasConceptScore W4310241418C2777119855 @default.
- W4310241418 hasConceptScore W4310241418C2778145968 @default.
- W4310241418 hasConceptScore W4310241418C2780026642 @default.
- W4310241418 hasConceptScore W4310241418C2780327821 @default.
- W4310241418 hasConceptScore W4310241418C2780581805 @default.
- W4310241418 hasConceptScore W4310241418C49105822 @default.
- W4310241418 hasConceptScore W4310241418C54355233 @default.
- W4310241418 hasConceptScore W4310241418C55493867 @default.
- W4310241418 hasConceptScore W4310241418C86803240 @default.
- W4310241418 hasIssue "1" @default.
- W4310241418 hasLocation W43102414181 @default.
- W4310241418 hasLocation W43102414182 @default.
- W4310241418 hasLocation W43102414183 @default.