FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4310253344> ?p ?o ?g. }

• W4310253344 endingPage "107869" @default.
• W4310253344 startingPage "107869" @default.
• W4310253344 abstract "Long double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) has been a well-known mechanism against white spot syndrome virus (WSSV) in cultured shrimp. In the present study, we investigated the protective efficacy of dsRNAs targeting the ribonucleotide reductase 2 (rr2) gene of WSSV according to length and target sequence location. To produce different lengths of dsRNAs, the 640 bp rr2 fragment (fragment I) was split into two equal 320 bp fragments (fragment II and III), then each 320 bp fragment was redivided into two 160 bp fragments (fragment IV, V, VI, and VII). After the synthesis of seven kinds of dsRNA fragments, dsRNAs with the same length were mixed with each other, then used for the evaluation of dsRNA’s length effect in Penaeus vannamei. The result showed that 160 bp long dsRNAs were as effective as 320 and 640 bp long dsRNAs in the protection of shrimp against WSSV infection, suggesting that the dsRNA length of 160 bp would be enough to be used as RNAi-mediated WSSV suppression in P. vannamei. However, as the 160 bp long dsRNAs used in the length effect experiment were not a single dsRNA population but a mixture of 160 bp dsRNA fragments covering the parent 640 bp long dsRNA, the sequence effect was not included in this RNAi efficacy. In the experiments to know the effect of not only length but also sequence of rr2-targeting long dsRNAs on the protective efficacy against WSSV, dsRNAs with a length of 640 bp (fragment I) and 320 bp (fragment II, III) showed a constant high defense ability, but the protection degree of long dsRNAs with a length of 160 bp was different depending on the kinds of the fragment, suggesting that the RNAi efficacy of some rr2-targeting long dsRNAs with a length of 160 bp might have sequences that are variable according to experimental conditions. In conclusion, this study showed that the protective ability of long dsRNAs in shrimp against WSSV infection can be affected by the length and sequence of the long dsRNAs." @default.
• W4310253344 created "2022-11-30" @default.
• W4310253344 creator A5017074883 @default.
• W4310253344 creator A5081232715 @default.
• W4310253344 date "2023-02-01" @default.
• W4310253344 modified "2023-09-25" @default.
• W4310253344 title "Effects of length and sequence of long double-stranded RNAs targeting ribonucleotide reductase 2 of white spot syndrome virus (WSSV) on protective efficacy against WSSV" @default.
• W4310253344 cites W1647075334 @default.
• W4310253344 cites W1916491745 @default.
• W4310253344 cites W1970592928 @default.
• W4310253344 cites W1986088405 @default.
• W4310253344 cites W1989746162 @default.
• W4310253344 cites W2010164218 @default.
• W4310253344 cites W2021168372 @default.
• W4310253344 cites W2030489085 @default.
• W4310253344 cites W2039796462 @default.
• W4310253344 cites W2040697674 @default.
• W4310253344 cites W2048217707 @default.
• W4310253344 cites W2060122336 @default.
• W4310253344 cites W2065000237 @default.
• W4310253344 cites W2090407901 @default.
• W4310253344 cites W2096251858 @default.
• W4310253344 cites W2145638174 @default.
• W4310253344 cites W2172377009 @default.
• W4310253344 cites W2345559765 @default.
• W4310253344 cites W2553382251 @default.
• W4310253344 cites W2605716503 @default.
• W4310253344 cites W2607234974 @default.
• W4310253344 cites W2630925036 @default.
• W4310253344 cites W2747957382 @default.
• W4310253344 cites W2767724162 @default.
• W4310253344 cites W2896014506 @default.
• W4310253344 cites W3040513224 @default.
• W4310253344 cites W3047097885 @default.
• W4310253344 cites W3094835272 @default.
• W4310253344 cites W4211039187 @default.
• W4310253344 doi "https://doi.org/10.1016/j.jip.2022.107869" @default.
• W4310253344 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36455669" @default.
• W4310253344 hasPublicationYear "2023" @default.
• W4310253344 type Work @default.
• W4310253344 citedByCount "0" @default.
• W4310253344 crossrefType "journal-article" @default.
• W4310253344 hasAuthorship W4310253344A5017074883 @default.
• W4310253344 hasAuthorship W4310253344A5081232715 @default.
• W4310253344 hasConcept C104317684 @default.
• W4310253344 hasConcept C153911025 @default.
• W4310253344 hasConcept C159047783 @default.
• W4310253344 hasConcept C166703698 @default.
• W4310253344 hasConcept C2191507 @default.
• W4310253344 hasConcept C2522874641 @default.
• W4310253344 hasConcept C2779771762 @default.
• W4310253344 hasConcept C54355233 @default.
• W4310253344 hasConcept C67705224 @default.
• W4310253344 hasConcept C86803240 @default.
• W4310253344 hasConceptScore W4310253344C104317684 @default.
• W4310253344 hasConceptScore W4310253344C153911025 @default.
• W4310253344 hasConceptScore W4310253344C159047783 @default.
• W4310253344 hasConceptScore W4310253344C166703698 @default.
• W4310253344 hasConceptScore W4310253344C2191507 @default.
• W4310253344 hasConceptScore W4310253344C2522874641 @default.
• W4310253344 hasConceptScore W4310253344C2779771762 @default.
• W4310253344 hasConceptScore W4310253344C54355233 @default.
• W4310253344 hasConceptScore W4310253344C67705224 @default.
• W4310253344 hasConceptScore W4310253344C86803240 @default.
• W4310253344 hasFunder F4320322030 @default.
• W4310253344 hasFunder F4320322120 @default.
• W4310253344 hasFunder F4320328359 @default.
• W4310253344 hasLocation W43102533441 @default.
• W4310253344 hasLocation W43102533442 @default.
• W4310253344 hasOpenAccess W4310253344 @default.
• W4310253344 hasPrimaryLocation W43102533441 @default.
• W4310253344 hasRelatedWork W1487857142 @default.
• W4310253344 hasRelatedWork W1968773193 @default.
• W4310253344 hasRelatedWork W1992579553 @default.
• W4310253344 hasRelatedWork W2042482406 @default.
• W4310253344 hasRelatedWork W2383970420 @default.
• W4310253344 hasRelatedWork W2568400328 @default.
• W4310253344 hasRelatedWork W2605716503 @default.
• W4310253344 hasRelatedWork W2962544630 @default.
• W4310253344 hasRelatedWork W3142757776 @default.
• W4310253344 hasRelatedWork W4280586978 @default.
• W4310253344 hasVolume "196" @default.
• W4310253344 isParatext "false" @default.
• W4310253344 isRetracted "false" @default.
• W4310253344 workType "article" @default.