Matches in SemOpenAlex for { <https://semopenalex.org/work/W4310289005> ?p ?o ?g. }
- W4310289005 endingPage "e202201801" @default.
- W4310289005 startingPage "e202201801" @default.
- W4310289005 abstract "Post-transcriptional gene silencing using double-stranded RNA has revolutionized the field of functional genetics, allowing fast and easy disruption of gene function in various organisms. In Drosophila, many transgenic RNAi lines have been generated in large-scale efforts, including the Drosophila Transgenic RNAi Project (TRiP), to facilitate in vivo knockdown of virtually any Drosophila gene with spatial and temporal resolution. The available transgenic RNAi lines represent a fundamental resource for the fly community, providing an unprecedented opportunity to address a vast range of biological questions relevant to basic and biomedical research fields. However, caution should be applied regarding the efficiency and specificity of the RNAi approach. Here, we demonstrate that pVALIUM10-based RNAi lines, representing ∼13% of the total TRiP collection (1,808 of 13,410 pVALIUM TRiP-based RNAi lines), cause unintended off-target silencing of transgenes expressed from Gateway destination vectors. The silencing is mediated by targeting attB1 and attB2 sequences generated via site-specific recombination and included in the transcribed mRNA. Deleting these attB sites from the Gateway expression vector prevents silencing and restores expected transgene expression." @default.
- W4310289005 created "2022-11-30" @default.
- W4310289005 creator A5045044289 @default.
- W4310289005 creator A5066212183 @default.
- W4310289005 creator A5070522126 @default.
- W4310289005 date "2022-11-29" @default.
- W4310289005 modified "2023-09-26" @default.
- W4310289005 title "<i>Drosophila</i>pVALIUM10 TRiP RNAi lines cause undesired silencing of Gateway-based transgenes" @default.
- W4310289005 cites W1961449438 @default.
- W4310289005 cites W1972937465 @default.
- W4310289005 cites W1973394316 @default.
- W4310289005 cites W1993903060 @default.
- W4310289005 cites W1994528729 @default.
- W4310289005 cites W2002256585 @default.
- W4310289005 cites W2010791234 @default.
- W4310289005 cites W2013727495 @default.
- W4310289005 cites W2020536597 @default.
- W4310289005 cites W2023447954 @default.
- W4310289005 cites W2036156074 @default.
- W4310289005 cites W2041330970 @default.
- W4310289005 cites W2045066366 @default.
- W4310289005 cites W2070441840 @default.
- W4310289005 cites W2080041937 @default.
- W4310289005 cites W2091998897 @default.
- W4310289005 cites W2096454034 @default.
- W4310289005 cites W2100208451 @default.
- W4310289005 cites W2100581989 @default.
- W4310289005 cites W2110643873 @default.
- W4310289005 cites W2111372229 @default.
- W4310289005 cites W2112038940 @default.
- W4310289005 cites W2114406978 @default.
- W4310289005 cites W2121665844 @default.
- W4310289005 cites W2148463363 @default.
- W4310289005 cites W2150972545 @default.
- W4310289005 cites W2158626657 @default.
- W4310289005 cites W2166903671 @default.
- W4310289005 cites W2264871965 @default.
- W4310289005 cites W2340421620 @default.
- W4310289005 cites W2397943578 @default.
- W4310289005 cites W2529998812 @default.
- W4310289005 cites W2539301705 @default.
- W4310289005 cites W2727701120 @default.
- W4310289005 cites W2781594154 @default.
- W4310289005 cites W2819760193 @default.
- W4310289005 cites W2899698612 @default.
- W4310289005 cites W2913346540 @default.
- W4310289005 cites W2953260789 @default.
- W4310289005 cites W2964675522 @default.
- W4310289005 cites W2973438117 @default.
- W4310289005 cites W3211446636 @default.
- W4310289005 cites W4205748228 @default.
- W4310289005 cites W4280626740 @default.
- W4310289005 cites W4304481530 @default.
- W4310289005 doi "https://doi.org/10.26508/lsa.202201801" @default.
- W4310289005 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36446522" @default.
- W4310289005 hasPublicationYear "2022" @default.
- W4310289005 type Work @default.
- W4310289005 citedByCount "1" @default.
- W4310289005 countsByYear W43102890052023 @default.
- W4310289005 crossrefType "journal-article" @default.
- W4310289005 hasAuthorship W4310289005A5045044289 @default.
- W4310289005 hasAuthorship W4310289005A5066212183 @default.
- W4310289005 hasAuthorship W4310289005A5070522126 @default.
- W4310289005 hasBestOaLocation W43102890051 @default.
- W4310289005 hasConcept C102230213 @default.
- W4310289005 hasConcept C104317684 @default.
- W4310289005 hasConcept C119056186 @default.
- W4310289005 hasConcept C136961756 @default.
- W4310289005 hasConcept C141231307 @default.
- W4310289005 hasConcept C166703698 @default.
- W4310289005 hasConcept C173396325 @default.
- W4310289005 hasConcept C190232843 @default.
- W4310289005 hasConcept C2191507 @default.
- W4310289005 hasConcept C25365240 @default.
- W4310289005 hasConcept C2780104201 @default.
- W4310289005 hasConcept C54355233 @default.
- W4310289005 hasConcept C67705224 @default.
- W4310289005 hasConcept C70721500 @default.
- W4310289005 hasConcept C86803240 @default.
- W4310289005 hasConcept C95444343 @default.
- W4310289005 hasConceptScore W4310289005C102230213 @default.
- W4310289005 hasConceptScore W4310289005C104317684 @default.
- W4310289005 hasConceptScore W4310289005C119056186 @default.
- W4310289005 hasConceptScore W4310289005C136961756 @default.
- W4310289005 hasConceptScore W4310289005C141231307 @default.
- W4310289005 hasConceptScore W4310289005C166703698 @default.
- W4310289005 hasConceptScore W4310289005C173396325 @default.
- W4310289005 hasConceptScore W4310289005C190232843 @default.
- W4310289005 hasConceptScore W4310289005C2191507 @default.
- W4310289005 hasConceptScore W4310289005C25365240 @default.
- W4310289005 hasConceptScore W4310289005C2780104201 @default.
- W4310289005 hasConceptScore W4310289005C54355233 @default.
- W4310289005 hasConceptScore W4310289005C67705224 @default.
- W4310289005 hasConceptScore W4310289005C70721500 @default.
- W4310289005 hasConceptScore W4310289005C86803240 @default.
- W4310289005 hasConceptScore W4310289005C95444343 @default.
- W4310289005 hasFunder F4320320879 @default.
- W4310289005 hasIssue "2" @default.